Kinetics and synthesis of poly(3-hydroxybutyrate) by a putative-mutant of Bacillus licheniformis

Ali, Sikander; Lodhi, Faiza Shabbir; Ahmad, M. Usman; Khan, Qaiser Farid; Asad-ur-Rehman; Ahmed, Abeera; Liaqat, Iram; Aftab, M. Nauman; Shah, Tawaf Ali; Salamatullah, Ahmad Mohammad; Wondmie, Gezahign Fentahun; Bourhia, Mohammed

doi:10.1186/s40643-024-00750-y

Bioresources and Bioprocessing

Table 2 Comparison of different kinetic parameters for PHB production by wild-type (IIB-isl9) and mutant strain (NA-cys4) of B. licheniformis

From: Kinetics and synthesis of poly(3-hydroxybutyrate) by a putative-mutant of Bacillus licheniformis

Kinetic parameters	Different incubation periods (h)
	24		48		72		96
	IIB-isl9	NA-cys4	IIB-isl9	NA-cys4	IIB-isl9	NA-cys4	IIB-isl9	NA-cys4
Yield coefficients
Growth yield coefficients
Y_x/s (g biomass/g)	2.062 ± 0.10	1.231 ± 0.06	1.583 ± 0.08	1.132 ± 0.06	0.897 ± 0.04	1.031 ± 0.05	0.511 ± 0.02	0.709 ± 0.04
Y_s/x (g/g biomass)	0.485 ± 0.02	0.813 ± 0.04	0.632 ± 0.03	0.883 ± 0.04	1.115 ± 0.06	0.972 ± 0.05	1.961 ± 0.10	1.411 ± 0.07
Product yield coefficients
Y_p/x (g/g biomass)	0.428 ± 0.02	1.091 ± 0.05	0.531 ± 0.03	1.254 ± 0.06	0.532 ± 0.03	1.238 ± 0.06	0.393 ± 0.02	1.411 ± 0.07
Y_p/s (g/g)	0.882 ± 0.04	1.341 ± 0.07	0.841 ± 0.04	1.421 ± 0.07	0.477 ± 0.02	1.274 ± 0.06	0.201 ± 0.01	1.001 ± 0.05
Specific rate constants
Specific rate constants for product formation
q_p*¹ (g/g/h)	0.064 ± 0.03	0.066 ± 0.03	0.044 ± 0.02	0.191 ± 0.01	0.011 ± 0.01	0.093 ± 0.05	0.002 ± 0.01	0.039 ± 0.01
q_p*² (g/g/h)	0.031 ± 0.02	0.212 ± 0.01	0.028 ± 0.01	0.168 ± 0.01	0.012 ± 0.01	0.091 ± 0.04	0.003 ± 0.01	0.055 ± 0.03
Specific rate constants for biomass development and glucose consumption
q_x (g/g/h)	0.148 ± 0.01	0.239 ± 0.01	0.082 ± 0.04	0.152 ± 0.01	0.021 ± 0.01	0.075 ± 0.03	0.004 ± 0.01	0.028 ± 0.01
q_s (g/g/h)	0.035 ± 0.01	0.158 ± 0.01	0.033 ± 0.02	0.118 ± 0.01	0.026 ± 0.01	0.071 ± 0.04	0.016 ± 0.01	0.055 ± 0.03
Volumetric rates
Volumetric rates for product formation
Q_p(g/l/h)	0.072 ± 0.01	0.194 ± 0.01	0.052 ± 0.03	0.134 ± 0.01	0.023 ± 0.01	0.073 ± 0.04	0.008 ± 0.01	0.039 ± 0.01
Volumetric rates for biomass development and glucose consumption
Q_x (g biomass/l/h)	0.168 ± 0.01	0.178 ± 0.01	0.097 ± 0.05	0.107 ± 0.01	0.043 ± 0.02	0.059 ± 0.03	0.021 ± 0.01	0.028 ± 0.01
Q_s (g/l/h)	0.081 ± 0.04	0.145 ± 0.01	0.061 ± 0.03	0.095 ± 0.05	0.048 ± 0.02	0.057 ± 0.03	0.041 ± 0.02	0.039 ± 0.02

Yx/s (g biomass/g) = dX/dS, Ys/x (g/g biomass) = dS/dX, Yp/x (g/g biomass) = dP/dX, Yp/s (g/g) = dP/dS. qp (g/g/h) = *1(µ × Yp/s), *2(µ × Yp/x), qx(g/g/h) = µ × Yx/s, qs(g/g/h) = µ × Ys/x. QP (g/l/h) = Slope of product formation, Qx (g biomass/l/h) = Slope of biomass development vs. incubation period and Qs (g/l/h) = Slope of glucose consumption vs. incubation period
µ h-1 = specific growth rate, calculated by plots of ln(X) vs. incubation period
±Indicate standard deviation (sd set at 5%) amongst the values of three parallel replicates. The sum mean values differ significantly at p ≤ 0.05 from each other in one-set, under one-way ANOVA

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