Fig. 2
From: Efficient expression of an alkaline pectin lyase from Bacillus licheniformis in Pichia pastoris
Enzymatic properties of PMGL-Ba. (A) SDS-PAGE analysis; 1:Purified fermentation supernatant; 2:Fermentation supernatant. (B) Determination of optimum pH (incubation in pH 5–11 buffer for 1 h). (C) pH stability (purified enzymes were incubated between pH 5.0–12.0 at 4℃ for 12 h). (D) Determination of optimum temperature (in a range of 50℃-70℃ in pH 8.0). (E) Temperature stability (Incubate in Tris-HCl buffer (pH 8.0) at 30–90℃ for 1 h). (F) PMGL-Ba temperature half-life