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Bioresources and Bioprocessing
Fig. 1 | Bioresources and Bioprocessing

Fig. 1

From: Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum

Fig. 1

Construction of a non-auxotrophic l-threonine producing C. glutamicum. The point mutations T311I and G378E were employed to release LysC and Hom from feedback inhibition by l-lysine and l-threonine, respectively. The native genes were replaced with genes SpdapA and EcilvA encoding heterologous enzymes with strong feedback inhibition, respectively. LysC, aspartate kinase; Hom, homoserine dehydrogenase; SpDapA, dihydrodipicolinate synthase from Streptococcus pneumoniae; IlvA, threonine dehydratase from E. coli. THF, tetrahydrofolate; Methyl-THF, 5,10-methylene tetrahydrofolate

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