Skip to main content
Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum

Fig. 3

Removal of feedback inhibition and overexpression of multiple key genes for improving l-threonine production. The 24-deep-well plate fermentation of engineered strains at 30 °C for 24 h. ZcglT1, C. glutamicum ATCC 13,032 derivative with T311I mutantion of lysC and G378E mutantion of hom; ZcglT2, ZcglT1 derivative with insertion of a constitutive promoter PgpmA−16 in front of homG378E; ZcglT3, ZcglT2 derivative with insertion of a constitutive promoter Ppyc−20 in front of lysCT311I. Thr, l-threonine; Lys, l-lysine; Ile, l-isoleucine; Gly, glycine; Hse, l-homoserine. Data are presented as mean values +/− SD (n = 3 independent experiments). ***P < 0.001, student’s two-tailed t-test

Back to article page