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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Reconstruction the feedback regulation of amino acid metabolism to develop a non-auxotrophic l-threonine producing Corynebacterium glutamicum

Fig. 5

Replacing the native CgilvA gene with EcilvA in ZcglT4 to reduce the by-product l-isoleucine. The 24-deep-well plate fermentation of engineered strains was performed at 30 °C for 24 h. ZcglT5, ZcglT4 derivative with replacement of the native CgilvA with EcilvA. Thr, l-threonine; Lys, l-lysine; Ile, l-isoleucine; Gly, glycine; Hse, l-homoserine. Data are presented as mean values +/− SD (n = 3 independent experiments). ***P < 0.001, student’s two-tailed t-test

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