Table 1 Strains used in this study
Strain | Description | Reference or sourcea |
|---|---|---|
E. coli | ||
Trans1-T1 | General cloning host | TransGen Biotech |
C. glutamicum | ||
ATCC 13,032 | Wild-type strain | ATCC |
ZcglT1 | ATCC 13,032 derivative with T311I mutantion of lysC and G378E mutantion of hom | This study |
ZcglT2 | ZcglT1 derivative with insertion of a constitutive promoter PgpmA−16 in front of homG378E | This study |
ZcglT3 | ZcglT2 derivative with insertion of a constitutive promoter Ppyc−20 in front of lysCT311I | This study |
ZcglT4 | ZcglT3 derivative with replacement of the native CgdapA with SpdapA | This study |
ZcglT5 | ZcglT4 derivative with replacement of the native CgilvA with EcilvA | This study |
ZcglT6 | ZcglT5 derivative harboring pEC-thrE plasmid | This study |
ZcglT7 | ZcglT5 derivative harboring pEC-serE plasmid | This study |
ZcglT8 | ZcglT5 derivative harboring pEC-rhtC plasmid | This study |
ZcglT9 | ZcglT5 derivative harboring pEC-rhtCthrB plasmid | This study |