Enhancing the biosynthesis of polyunsaturated fatty acids by Rhodotorula mucilaginosa and Lodderomyces elongisporus

Bioresources and Bioprocessing

Table 1 Evaluation of lipid synthesis using the SPV test in two yeast isolates cultured for 20 days at three different temperatures (7, 15, and 26 °C) in HGM

Incubation temperature	Yeast	Days	Slope 1	Slope 2	Slope 3	Correlation R²
(a) 7 °C	R. mucilaginosa	5	0.6098	0.3084	0.0161	0.9046
		10	1.4432	0.7299	0.0381	0.9866
		15	1.4356	0.7261	0.0379	0.9996
		20	0.5947	0.3004	0.0157	0.9431
	L. elongisporus	5	0.4773	0.2414	0.0126	0.961
		10	1.9621	0.9923	0.0518	0.9587
		15	1.3485	0.682	0.0356	0.9166
		20	0.4773	0.2414	0.0126	0.9041
(b) 15 °C	R. mucilaginosa	5	0.6553	0.3314	0.0173	0.9958
		10	0.7273	0.3678	0.0192	0.9799
		15	1.6061	0.8123	0.0424	0.9232
		20	1.4735	0.7452	0.0389	0.9892
	L. elongisporus	5	0.8258	0.4176	0.0218	0.8594
		10	0.9015	0.4559	0.0238	0.9776
		15	1.3258	0.6705	0.035	0.9838
		20	1.4356	0.7261	0.0379	0.9378
(c) 26 °C	R. mucilaginosa	5	0.7765	0.3927	0.0205	0.9453
		10	2.5909	1.3103	0.0684	0.9588
		15	1.1591	0.5862	0.0306	0.9717
		20	1.7045	0.8621	0.045	0.9843
	L. elongisporus	5	0.6477	0.3276	0.0171	0.9601
		10	0.4583	0.2318	0.0121	0.9936
		15	1.1023	0.5575	0.0291	0.9395
		20	1.1098	0.5613	0.0293	0.9902

Fish oil and Flax seed oil were used as external standards for the conversion of absorbance units to µg of lipids. Slope 1; from linear equation using Flax seed oil as an external standard. Slope 2; from linear equation using Fish oil as an external standard. Slope 3; from linear equation of SPV-microassay (absorbance measured at 570 nm)