Enhancing the biosynthesis of polyunsaturated fatty acids by Rhodotorula mucilaginosa and Lodderomyces elongisporus

Bioresources and Bioprocessing

Table 3 The influence of temperature on the growth and lipid synthesis of R. mucilaginosa and L. elongisporus

Organism	Temp. (°C)	Day	Medium	Y_L/s (mg/g)	Y_L/x (mg/mg)	Y_x/s (mg/g)	Biomass (mg/mL)	Lipids (mg/mL)
R. mucilaginosa	7 °C	15	HGM	0.16	0.58	0.27	21.5	12.4
	15 °C	20	HGM	0.07	0.38	0.18	14.7	5.61
	26 °C	20	HGM	0.07	0.42	0.17	13.3	5.59
	26 °C	28	HGM + VIT	0.14	0.49	0.28	22.6	11.1
L. elongisporus	7 °C	15	HGM	0.17	0.66	0.26	20.4	13.4
	15 °C	20	HGM	0.06	0.37	0.15	11.9	4.41
	26 °C	20	HGM	0.08	0.48	0.17	13.3	6.39
	26 °C	28	HGM + VIT	0.18	0.53	0.34	27	14.2

R. mucilaginosa and L. elongisporus were cultured at different temperatures in HGM and HGM + VIT containing 80 g/L glucose. the ultimate concentrations of biomass and lipids, Y_X/S, Y _L/S, and Y_L/X, are biomass/glucose, lipid/glucose, and lipid/biomass yield coefficients, respectively, these coefficients altered with growing temperatures