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Table 1 Design of the experiment

From: Nutrients, surfactants, and aeration in constructed wetlands affect bacterial persistence and metabolic activity during the remediation of crude oil-contaminated water

Treatment

Experimental design

Control (C1)

Mesocosm with vegetation, without crude oil and bacterial augmentation

Control (C2)

Mesocosm having crude oil without vegetation and bacterial augmentation

T1

Crude oil spiked CWs vegetated with T. latifolia (without bacterial augmentation)

T2

Crude oil spiked CWs vegetated with T. latifolia, and augmented with selected bacteria

T3

Crude oil spiked CWs vegetated with T. latifolia, augmented with selected bacteria and externally supplied with nutrients, surfactant and aeration

T4

Crude oil spiked CWs vegetated with C. laevigatus (without bacterial augmentation)

T5

Crude oil spiked CWs vegetated with C. laevigatus, and augmented with selected bacteria

T6

Crude oil spiked CWs vegetated with C. laevigatus, augmented with selected bacteria and externally supplied with nutrients, surfactant and aeration