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Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction



Biopalladium (bioPd(0)) nanoparticles on Klebsiella Pneumoniae ECU-15 were synthesized mainly on the microorganism's surface. Data suggest that the resistance of mass transfer around the cell surface region plays a critical role in bioPd(0) synthesis process. However, the mechanisms for its role remains elusive.


The experimental results indicated that 1) diffusion resistance existed around the microorganism's cell in reaction vessel and 2) fluid shear stress affected the mass transfer rates differently according to its strength and thus had varying effects on the bioPd(0) synthesis. More than 97.9 ± 1.5% Chromium(VI)(Cr(VI)) (384 μM) was reduced to Cr(III) within 20 min with 5% Pd/bioPd(0) as catalyst, which was generated by the K. Pneumoniae ECU-15, and the catalytic performance of Pd/bioPd(0) was stable over 6 months. The optimal condition of bioreduction of Pd(II) to Pd(0) was determined at the Kolmogorov eddy length of 7.33 ± 0.5 μm and lasted for 1 h in the extended reduction process after the usual adsorption and reduction process.


It is concluded that a high bioPd(0) catalytic activity can be achieved by controlling the fluid shear stress intensity in an extended reduction process in the bioreactor.


The recovery of nanopalladium particles from waste has been of great interest recently [1]. Certain physical properties of nanopalladium particles differ from those of the bulk material [2],[3]. Palladium is used extensively because of its catalytic activity in some chemical reactions. Traditionally, the preparation of nanopalladium requires the rigorous experimental procedures. In contrast, the microbiological biosynthetic process of converting Pd(II) to Pd(0) works through the binding of metal Pd(II) ions onto highly reactive bacterial cell surfaces concomitant with Pd(II) ion reduction [4] at ambient temperature and pressure. This process does not require hazardous chemicals and, thus, is effective and environmentally friendly.

Many kinds of bacterial species have been used in the reduction of Pd(II) salts to their elementary metallic form [1]. Humphries et al. investigated the reduction of Cr(chromium(VI) by immobilized cells of Desulfovibrio vulgairs NCIMB8303 and Microbacterium sp. NCIMB 13776, which showed that the best immobilization matrices were 130 (agarose) and 15 (agar) nmol h−1 mg dry cell wt−1, with the highest Cr(VI) reducing efficiency [5]. Macaskie et al. studied palladium catalysts generated on gram negative (Desulfovibrio) and gram positive (Bacillus) bacterial surfaces. Discrete nanoparticles were located in the periplasmic space of Desulfovibrio desulfuricans[6] and in the peptidoglycan and proteinaceous surface layer (S-layer) of Bacillus sphaericu[7]. De Windt et al. found that the bioreductive deposition of Pd(0) occurred on the cell wall and in the periplasmic space of Shewanella oneidensis in the presence of a series of electron donors [8]. Deplanche et al. investigated the involvement of hydrogenase in the formation of Pd(0) using Escherichia coli mutant strains [9]. Martins et al. found that a Pd(II) resistant bacterial community could biorecover this metal from a solution, and that the bacterial consortium was closely related to several Clostridium species, Bacteroides, and Citrobacter[10]. Many fermentative species could produce hydrogen during fermentation and subsequently reduce Pd(II) to Pd(0). An addition of Pd(II) to the fermenting culture of Clostridium pasteurianum could result in the formation of Pd(0) nanoparticles on the bacterial cell wall and in the cytoplasm [11]. Klebsiella pneumoniae is a potential biohydrogen producer [12] and has the ability to bioreduce Pd(II) [13]. The evaluation of the capacity of K. pneumoniae to reduce Pd(II) to Pd(0) nanoparticles would be significant because of its respiration diversity, its wide distribution in various environments, and its potentially effective approach to the remediation of aggressive metal waste.

Biopalladium nanoparticles were synthesized mainly inside the cell surface region [14]. The resistance to mass transfer inside this region during bioPd(0) preparation should not be neglected. Han et al. found that besides the heating effect, microwaves could also enhance the mass transfer rates of active constituents. The bound potential of cell walls could be overcome by a certain microwave intensity to increase the extraction efficiency [15]. The fluid shear stress could also be used to open the periplasmic layer, reduce its mass transfer resistance and enhance the rates [16]. However, the influence of shear stress on the chemical and physical properties of biosynthetic nanopalladium particles was not fully understood. In this study, the influence of fluid shear stress during the bioPd(0) preparation process on its catalytic activity for Cr(VI) reduction to Cr(III) was investigated. Physical properties of the bioPd(0) were measured by a transmission electron microscopy (TEM), energy-dispersive X-ray spectroscopy (EDAX), and X-ray diffractometry (XRD). The results would be significant for the development of the efficient and stable bioPd(0) catalyst production biotechnology.


Preparation of the bioPd(0) catalysts

K. pneumoniae ECU-15 was isolated from the anaerobic sewage sludge [12] and cultured to a high-cell concentration in an experimental reactor. The biomass at mid-logarithmic phase cultures was harvested by a centrifuge (4000 g, 15 min, 4°C). The pellets were washed three times by 100 mL of MOPS-NaOH buffer (20 mM, pH 7.2) and resuspended in 50 mL of the same buffer. And then it was stored at 4°C under N2 for no more than 24 h until use. Cell concentration (mg/mL) was determined by a correlation to a predetermined OD600 to dry weight conversion.

A known volume of the concentrated resting cell suspension was transferred anaerobically into a 100-mL serum bottle, which contained an appropriate volume of degassed 2-mM PdCl2 solution to make the final weight ratio of Pd to dry cells equal to 1:19. That means a final loading of 5% Pd on biomass could be obtained. A series of steps was scheduled as follows: firstly, the static adsorption process between the microorganism and the Pd(II) lasted for 30 min, then the solution was sparged by H2 for 20 min in the reduction process. The H2 was retained in the serum bottle till the end of the preparation process. The degree of the Pd(II) reduction from the solution was confirmed by assaying the residual Pd(II) ion concentration by the SnCl2 method in cell/Pd mixture supernatant [17]. The Pd(0)-coated biomass was harvested by centrifugation(4,000 g, 15 min, 25°C) and rinsed three times by a distilled water (dH2O). The black precipitate was washed once by acetone and left to dry in air for overnight to a constant weight and finely ground in a mortar. The physical properties of the Pd-loaded bacterial cells were measured using TEM (JEM-2100, JEOL, Akishima-shi, Japan), EDAX (DX-4, EDAX, Mahwah, NJ, USA), and the XRD (D/max2550B/PC, Rigaku, Shibuya-ku, Japan). The catalytic activity of the bioPd(0) was tested without further processing.

Transmission electron microscopy

The bacteria loaded with the palladium were rinsed twice with the distilled water and then fixed in the 2.5% glutaraldehyde. After being centrifuged at 10,000 rpm, the pellets of palladium-loaded bacteria were resuspended in 15 mL of 0.1 M Na-cacodylate buffer and then stained for an hour in the 1% osmium tetroxide solution in 0.1 M phosphate buffer at pH 7 for the measurement of the TEM. Cells were dehydrated for 15 min by the 70%, 90%, and 100% ethanol solution, respectively. Then, it was washed twice in the propylene oxide for 15 min. The cells were embedded in the epoxy resin and then the mixture was left to be polymerized at 60°C for 24 h. A section (100-nm thick) was cut from the resin block, placed onto a copper grid, and then viewed with TEM (JEM-2100, JEOL, Akishima-shi, Japan).

EDAX and XRD measurements

For the EDAX and the XRD analysis, the parallel samples of Pd(0)-loaded biomass were washed for three times in the distilled water and once in acetone. After the centrifugation process, the Pd(0)-loaded biomass were resuspended in a small volume of acetone and then air-dried to a constant weight. Finally, it was grounded to a fine powder by an agate mortar. The powder was analyzed using the EDAX (DX-4, EDAX, Mahwah, NJ, USA) and the XRD (D/max2550B/PC, Rigaku, Shibuya-ku, Japan). The powder pattern was compared to the references in the Joint Committee for Powder Diffraction Studies (JCPDS) database.

Evaluation of catalytic activity via Cr(VI) reduction to Cr(III)

The bioPd(0) could be used as the catalyst for the reduction of Cr(VI) to Cr(III). In this paper, a comparative study of the catalytic activity of bioPd(0) under various condition of preparation was carried out. During the experimental process, 10 mg bioPd(0) were accurately weighed and then put into a 25-mL serum bottle sealed with butyl rubber stopper, containing 10 mL, 384 μM Cr(VI). The mixture was made anaerobic by sparging the solution with oxygen free nitrogen for 10 min. The bottle was stalled in a 30°C water bath for 30 min before the reaction. The reaction was initiated when 1 mL of sodium formate as the electron donor was added with the final concentration to 25 mM. The samples were periodically withdrawn from the bottle via the rubber septa and centrifuged at 4,000 rpm for 2 min to remove the bioPd(0) catalyst. The supernatant was measured to determine the concentration of Cr(VI) and Cr(III).

Effect of different conditions on catalytic activity in the extended reduction of Cr(VI) to Cr(III)

Comparative studies of the catalytic activity of bioPd(0) for Cr(VI) reduction to Cr(III) were carried out under different bioPd(0) preparation conditions. The preparation of bioPd(0) nanoparticles through the reduction of Pd(II) by K. pneumoniae ECU-15 comprised three steps, as represented schematically in Figure 1. In the adsorption step, the Pd(II) ions in the solution were adsorbed on the cell surface. In the reduction step, the adsorbed Pd(II) ions were reduced to Pd(0) at the cell surface with hydrogen sparging. And in the third step, the reduction process was extended through the introduction of stationary or shaking factors, which represented the new findings of this paper.

Figure 1
figure 1

Schematic representation of the bioPd(0) synthesis.

Stationary operation in the extended reduction process referred to the cell/Pd(0)/Pd(II) mixture solution being allowed to stand from 0 to 13 h. BioPd(0) was harvested and used as Cr(VI) reduction catalyst after several hours of standing time. The variation in shaking factors referred to a variation in shaker speed from 50 to 200 rpm and time from 1 to 3 h only after hydrogen sparging. The reduction of Cr(VI) was estimated as the percent of Cr(VI) which were reduced to Cr(III) at the set time. The reduction ratio could be calculated from the following formulas:

η= C 0 C t C 0

Where C0 was the initial concentration of Cr(VI), and Ct was the concentration of Cr(VI) at time t.

Results and discussion

Examination of bioPd(0) samples by electron microscopy, energy dispersive X-ray spectroscopy, and X-ray diffraction

As shown in Figure 2, the color of the solution in the reduction system changed from milk white to black, which indicated that the Pd(0) might be generated through the reduction of Pd(II) ions by the K. pneumoniae ECU-15. After the centrifugation and rinse treatment, the black precipitate was dried to a constant weight and finely ground in a mortar, which was finally stored in the vacuum desiccator.

Figure 2
figure 2

Photographs of color during the process of Pd(II) reduction to Pd(0) with K. pneumoniae ECU-15. (a) Pd(II) solution, (b) Pd(II) and the K. pneumoniae suspension, and (c) bioPd(0) and the K. pneumoniae solution.

The TEM photographs of the Pd-located cells of the K. pneumoniae ECU-15 were shown in Figure 3a,b,c,d. The nanoPd(0) deposits were almost the round black particles with similar morphology. It was also shown that the Pd particles were distributed on the periplasmic space and the cytoderm of the K. pneumoniae ECU-15, which was consistent with that of the previous research [18]. It seems that there was no bioPd(0) appeared in the intercellular space, which indicated that the nano bioPd(0) might be generated by some catalytic reduction reaction on the cell surface of K. pneumoniae ECU-15. According to the various magnifications of the TEM images of the bioPd(0) nanoparticals, it seems that the particles size ranged from 5.0 to 26.5 nm, with the average article size of 15.8 nm. The identity of the nanoparticle deposits as bioPd(0) was confirmed by the EDAX analysis shown in Figure 3e, where the Pd components was obvious. The C, O, and P components shown in Figure 3e were the cellular material. The characteristic diffraction peak of the crystalline Pd(0) particles at 2θ = 40°, 47°, and 68° were also shown by the XRD diffractograms, as reported by Hennebel et al. [13]. It was also confirmed that the cell-mediated reduction of the Pd(II) to the metallic Pd(0), with a face-centered cubic structure [19],[20] through the XRD patterns of the Pd(II)-challenged cells, were shown in Figure 3f. All these results proved that the generation of the bioPd(0) was through the reduction of the Pd(II) to Pd(0) on the surface of the K. pneumoniae ECU-15 cells. The location of the bioPd(0) nanoparticles generated might be caused by the distribution and properties of hydrogenase and other enzymes involved in Pd(II) reduction [11]. However, Rotaru found that the cell surface functional groups really played a catalytic function role for the Pd(II) reduction to the bioPd(0) [3]. These mechanisms were not yet unified and needed to be explored carefully. The accepted view was that the reactant should firstly transfer from the solution to the function groups or the enzymes on the cell surface, and thus, the resistance of mass transfer inside the cell surface scaffold should not be neglected.

Figure 3
figure 3

Properties of bioPd(0) generated by Pd(II) reduction on the cell surface of the K. pneumoniae ECU-15. (a-d) The TEM of nanoparticles of Pd(0) at different scale; (e) The EDAX results of Pd(0); (f) The XRD patterns of Pd(0).

Effect of extended reduction standing time during bioPd(0) preparation on catalytic activity for Cr(VI) reduction to Cr(III)

The cell wall of K. pneumoniae ECU-15 is composed mainly of peptidoglycan, which contributes to the mechanical strength of the bacterial cell structure and prevents the intrusion of various ions as a barrier to mass transfer [21]. As discussed above, the synthesis process of bioPd(0) nanoparticles by the reduction of Pd(II) on K. pneumoniae ECU-15 included an adsorption process and a reduction process [20]. In order to confirm the time of the adsorption process, the adsorption efficiency of Pd(II) in the solution could be determined by detecting the residual Pd(II) concentration through the SnCl2 method. The preliminary experiments showed that the residual Pd(II) concentration gradually decreased within the first 30 min, and the adsorption equilibration of Pd(II) would be reached at 30 min. The influence of standing time on the extended reduction of Cr(VI) to Cr(III) by bioPd(0) catalytic activity was given in Figure 4. The catalytic activity was improved by extending the reduction time. An increase in standing time from 0 to 6 h resulted in the complete reduction of Cr(VI) to Cr(III) in 1 h instead of 5 h. Especially, the Cr(VI) reduction ratio(η) has increased exponentially in the first 1 h with the standing treatment. With a further increase in standing time, the improvement in catalytic activity of the bioPd(0) was not obvious. Extending the reduction time would influence the equilibrium time for bioPd(0) generation, which influence the bioPd(0) catalytic activity indirectly. The optimal standing time was found to be 6 h. The TEM micrograph in Figure 5 showed that the nanoparticles were distributed homogeneously with higher density in the periplasmic space and cytoderm with the longer standing time (Figure 5a,c), while in Figure 5b,d, the palladium particles demonstrated with the properties of uneven size and inhomogeneity distribution. This observation further confirmed the results in Figure 4.

Figure 4
figure 4

Effect of the standing time in the extending reduction process on the catalytic activity of bioPd(0).

Figure 5
figure 5

TEM photo of bioPd(0) at different standing time in the extending reduction process. TEM photo of bioPd(0) collected at a standing time of 6 h (a,c) and collected immediately (b,d) in the extending reduction process.

During the Pd(II) reduction process, the Pd(II) ions and molecular hydrogen in the solution would pass through several layers, such as the gas-liquid, liquid-liquid, and solid–liquid layers before arriving at the hydrogenase and functional organic groups on the membrane and periplasmic space of the cells [22]. It was followed by the nucleation of Pd(0) and further cluster growth. The three-dimensional structure of the periplasmic space around the bacterial cell had a certain resistance to the ions [23] and the molecular diffusion process and more time was required for the delivery of Pd(II) ions and molecular hydrogen to the microorganism cell surface to complete the reduction of Pd(II) to Pd(0). Thus, it’s necessary to overcome the resistance to mass transfer around the microgram cell for the efficient preparation of stable and high efficiency bioPd(0).

Effect of fluid shear stress during Pd(II) adsorption and reduction processes on the catalytic activity of bioPd(0) for Cr(VI) reduction to Cr(III)

It is critical that the Pd(II) ion and molecular hydrogen were transferred efficiently to the synthesis site of the bioPd(0) on the cell surface of K. pneumoniae ECU-15. Therefore, mass transfer problems in the preparation of bioPd(0) should be investigated. The fluid shear stress, which was generated by various flask shaking intensities, could reduce the mass transfer resistance in the periplasmic layer [23],[24]. The movement of the turbulent eddies, which were produced by fluid turbulence intensities, would generate fluid shear stress [25]. The turbulence intensity could be demonstrated by the Kolmogorov eddy length of the fluid [26]. For an internal fluid in the Erlenmeyer flask at different shaking speeds, the Kolmogorov eddy length of the fluid turbulence could be calculated from Equation (2) [27]. The estimated values for the energy dissipation rate and smallest eddy size at different shaking speeds were given in Table 1. When the shaking speeds increased from 50 to 200 rpm, the Kolmogorov eddy length would decrease from 160.69 to 3.26 μm. And thus, the impact of fluid shear force could be expressed quantitatively by the Kolmogorov eddy length.

λ = ν 3 ε 0.25 , ε = k ρ 1 V t 0.25 N 2.81
Table 1 Estimated values for energy dissipation rate and smallest eddy size of the reactor fitted at different speeds

where λ is the Kolmogorov eddy length (μm), v is the fluid kinematic viscosity (m2/s), ε is the unit average energy consumption power of the Erlenmeyer flask, k is the constant (1.09 × 10−3), ρ is the liquid density (kg/m3), V t is the working volume in the Erlenmeyer flask (m3), and N is the shaking speed of the Erlenmeyer flask (s−1).

Additional file 1: Figure S1 showed the recovery ratios of Pd(0) at different shaking speed. It seems that all the recovery of Pd(0) exceeded 90% with little difference shown among the various shaking speeds. The possible reason was that the Pd(II) was mainly reduced by H2, but the other factors, such as the fluid shear stress, the hydrogenase, etc., in the bioreduction process predominantly regulated and controled the physical properties of the Pd(0), which fundamentality influenced the catalytic activity of Pd(0). The effects of shaking speed during the adsorption and the reduction process on the catalytic activity of bioPd(0) for Cr(VI) reduction to Cr(III) were studied in this study with results shown in Figure 6a. The η of the Cr(VI) reduction to Cr(III) increased as the reaction time increased, and gradually added to a certain value. At the initial time of the reduction process, the reduced rates of Cr(VI) to Cr(III) increased exponentially by the bioPd(0) generated with the shaking treatment, which was obviously higher than those without shaking. After 3.5 h, 84.6% Cr(VI) was reduced to Cr(III) by the bioPd(0) generated at the shaking speed of 150 rpm. It reached the 95% with the bioPd(0) prepared at 200 rpm, while only 60% Cr(VI) was reduced with 300 rpm. It was indicated that the higher shear stress was beneficial to the synthesis of bioPd(0) with higher catalytic activity. However, too high shear stress, such as 300 rpm, would be harmful, which adversely affect the preparation of bioPd(0). Approximately 5.5 h was required for the accomplishment of the reduction of Cr(VI) to Cr(III) by the bioPd(0) generated at a shaking speed of 150 or 200 rpm in the generated process. The data suggested that the shaking during adsorption or reduction process did not obviously impact on the improvements in the catalytic activity of the bioPd(0). It was estimated that this process was reaction controlled, and the increase of the mass transfer rates would not visibly influence the bioPd(0) catalytic activity.

Figure 6
figure 6

The effects of fluid shear stress in the preparation to the catalytic activity of bioPd(0). The effect of the shaking in adsorption process and reduction process to catalytic activity of bioPd(0) (a). The effect of the shaking speed and the shaking time in the extending reduction process to the conversion of the reduction of Cr(VI) to Cr(III) with the bioPd(0) as catalyst (b). The constant of reaction rate of bioPd(0)s on the reduction of Cr(VI) to Cr(III) at different shaking speeds (c).

Effect of fluid shear stress during the extended Pd(II) reduction process on catalytic activity of bioPd(0) for Cr(VI) reduction to Cr(III)

The cell-Pd(II) mixed with liquor after 30 min adsorption was sparged with hydrogen for 20 min under static conditions before it was placed on a shaking table. As seen in Figure 6b, the η was studied as functions of the different shaking speeds. The catalytic activity of bioPd(0) on the η could be improved severalfold when the shaking speeds were increased from 50 to 200 rpm. The optimum reduction condition was determined at 75 min, 150 rpm. When the shaking speeds increased from 50 to 150 rpm, the catalytic activity of bioPd(0) increased proportionately. However, the catalytic activity of the bioPd(0) decreased at 200 rpm. The catalytic activity of bioPd(0) with shear stress was higher than that without. And then the influence of the shaking time on the catalytic activity in the extended reduction process was investigated. As seen in Additional file 1: Figure S2, there was no obvious improvement in the catalytic activity of bioPd(0) with the time extended. The effect of fluid shear stress on bioPd(0) catalytic activity varied among the adsorption, reduction, and extended reduction process. In the adsorption and reduction processes, more time was required for the dissolved hydrogen molecules to reach a certain concentration around the cell surface before the beginning of the bioPd(0) synthesized process; whereas in the extended reduction process, when the dissolved hydrogen molecule and Pd(II) concentrations in the periplasmic region reached a certain level, strengthening the mass transfer for generating biological palladium would have a significant effect on the catalytic activity of bioPd(0). Hence, it was speculated that the shaking might assist in the even dispersion of Pd(II) on or into the location of Pd(0) generated sites. The fluid shear stress in the extended reduction process could affect the catalytic activity of bioPd(0) significantly. A possible explanation was that this stage was mass transfer controlled, and strengthening the mass transfer would be beneficial to generate bioPd(0) with high catalytic activities [28]. It required the further research.

The diffusion rates of Pd(II) ions and hydrogen molecules from the solution to the liquid boundary layer surrounding the bacterial cell increased with fluid turbulence intensities. The thickness of the liquid boundary layer and the external diffusion resistance decreased with turbulence intensity and fluid shear stress [29]. The too high shear stress might reduce the enzyme activity and destroy the cellular structure [24],[30]. Small eddies, similar in size to those of the cells, could cause the higher shear stresses on the cells and lead to the physical damage [31]. As shown in Table 1, when the shaking speeds increased from 50 to 150 rpm, the smallest eddy sizes decreased from 160.69 to 7.33 μm, which was greater than the size of the K. pneumoniae ECU-15 cells (1–1.5 μm). However, when the shaking speed was 200 rpm, the smallest eddy size (3.26 μm) was close to that of the K. pneumoniae ECU-15 cells with the catalytic activity of the bioPd(0) for Cr(VI) reduction to Cr(III) decreased. A suitable fluid shear stress would probably decrease the mass transfer resistance with more bioPd(0) being nucleated and evenly distributed on the cells. Considering the time consumption and cost of resources, the optimal conditions for the preparation of high catalytic activity bioPd(0) were to shake the mixed liquor at 150 rpm during the extended reduction process.

More than 97.9 ± 1.5% Cr(VI) (384 μM) was reduced to Cr(III) within 20 min with 5% Pd/bioPd(0) as the catalyst. The catalytic performance of the bioPd(0) was stable over 6 months. The 5% Pd/carbon commercial catalyst could reduce 96 ± 1.3% Cr(VI)(500 μM) in 60 min [9]. It seems that the bioPd(0) generated from the fluid shear stress regulation process could be a competitive biocatalyst.

The Cr(VI) reaction ratio changed with different types of bioPd(0) catalyst and was solely attributed to the different shaking speeds in the extended reduction process. The reaction ratio constants (k) of Cr(VI) to Cr(III) with different types of bioPd(0) as catalysts were calculated from Equation (3). The slope of the lines in Figure 6c was the reaction ratio constant (k). A maximum k of 1.199 min−1 was obtained at 150 rpm. The difference in maximum k was mainly because of the maximum catalytic activity of the bioPd(0), which resulted from the different shaking speeds in the extended reduction process. A suitable shaking speed would reduce the reaction time of Cr(VI) to Cr(III) reduction process.

In 1 1 y =kt

where y is the percentage Cr(VI) consumption, t is the test time (s), and k is the reaction rate constant.


BioPd(0) was produced inside the periplasm of K. pneumoniae ECU-15 cells and could catalyze the reduction of Cr(VI) to Cr(III). Extending the standing time in the reduction process would be benefical for the biosynthesis of the bioPd(0) with high catalytic activity. Increasing the mass transfer rates through regulating the fluid shear stress for the extended reduction process during the bioPd(0) generation process would improve the catalytic activity of bioPd(0) for the reduction of Cr(VI) to Cr(III). The optimal condition of the bioreduction of Pd(II) to Pd(0) was at the Kolmogorov eddy length of 7.33 ± 0.5 μm, 1 h in the extended reduction process after the usual adsorption and reduction process. Increasing the mass transfer rate by the regulation of the fluid shear stress could provide certain advantages and possibilities for the development of bioreactor technologies for the noble metal recovery.

Additional file


  1. Yong P, Rowson NA, Farr JPG, Harris IR, Macaskie LE: Bioreduction and biocrystallization of palladium by Desulfovibrio desulfuricans NCIMB 8307. Biotechnol Bioeng 2002, 80: 369–379. 10.1002/bit.10369

    Article  CAS  Google Scholar 

  2. Hennebel T, De Gusseme B, Boon N, Verstraete W: Biogenic metals in advanced water treatment. Trends Biotechnol 2009, 27: 90–98. 10.1016/j.tibtech.2008.11.002

    Article  CAS  Google Scholar 

  3. Rotaru AE, Jiang W, Finster K, Skrydstrup T, Meyer RL: Non‐enzymatic palladium recovery on microbial and synthetic surfaces. Biotechnol Bioeng 2012, 109: 1889–1897. 10.1002/bit.24500

    Article  CAS  Google Scholar 

  4. Karthikeya S, Beveridge TJ: Pseudomonas aeruginosa biofilms react with and precipitate toxic soluble gold. Environ Microbiol 2002, 4: 667–675. 10.1046/j.1462-2920.2002.00353.x

    Article  Google Scholar 

  5. Humphries AC, Nott KP, Hall LD, Macaskie LE: Reduction of Cr(VI) by immobilized cells of Desulfovibrio vulgaris NCIMB 8303 and Microbacterium sp. NCIMB 13776. Environ Microbiol 2005, 90: 589–596.

    CAS  Google Scholar 

  6. Lloyd JR, Yong P, Macaskie LE: Enzymatic recovery of elemental palladium by using sulfate-reducing bacteria. Appl Environ Microbiol 1998, 64: 4607–4609.

    CAS  Google Scholar 

  7. Creamer NJ, Mikheenko IP, Yong P, Deplanche K, Sanyahumbi D, Wood J, Pollmann K, Merroun M, Selenska-Pobell S, Macaskie L: Novel supported Pd hydrogenation bionanocatalyst for hybrid homogeneous/heterogeneous catalysis. Catal Today 2007, 128: 80–87. 10.1016/j.cattod.2007.04.014

    Article  CAS  Google Scholar 

  8. De Windt W, Aelterman P, Verstraete W: Bioreductive deposition of palladium (0) nanoparticles on Shewanella oneidensis with catalytic activity towards reductive dechlorination of polychlorinated biphenyls. Environ Microbiol 2005, 7: 314–325. 10.1111/j.1462-2920.2005.00696.x

    Article  CAS  Google Scholar 

  9. Deplanche K, Caldelari I, Mikheenko IP, Sargent F, Macaskie LE: Involvement of hydrogenases in the formation of highly catalytic Pd (0) nanoparticles by bioreduction of Pd (II) using Escherichia coli mutant strains. Microbiol 2010, 156: 2630–2640. 10.1099/mic.0.036681-0

    Article  CAS  Google Scholar 

  10. Martins M, Assuncao A, Martins H, Matos AP, Costa MC: Palladium recovery as nanoparticles by an anaerobic bacterial community. J Chem Technol Biotechnol 2013, 88: 2039–2045.

    CAS  Google Scholar 

  11. Chidambaram D, Hennebel T, Taghavi S, Mast J, Boon N, Verstraete W, van der Lelie D, Fitts JP: Concomitant microbial generation of palladium nanoparticles and hydrogen to immobilize chromate. Environ Sci Technol 2010, 44: 7635–7640. 10.1021/es101559r

    Article  CAS  Google Scholar 

  12. Niu K, Zhang X, Tan WS, Zhu ML: Characteristics of fermentative hydrogen production with Klebsiella pneumoniae ECU-15 isolated from anaerobic sewage sludge. Int J Hydrogen Energy 2010, 35: 71–80. 10.1016/j.ijhydene.2009.10.071

    Article  CAS  Google Scholar 

  13. Hennebel T, Van Nevel S, Verschuere S, De Corte S, De Gusseme B, Cuvelier C, Fitts JP, Van der Lelie D, Boon N, Verstraete W: Palladium nanoparticles produced by fermentatively cultivated bacteria as catalyst for diatrizoate removal with biogenic hydrogen. Appl Microbiol Biotechnol 2011, 91: 1435–1445. 10.1007/s00253-011-3329-9

    Article  CAS  Google Scholar 

  14. Deplanche K, Merroun ML, Casadesus M, Tran DT, Mikheenko IP, Bennett JA, Zhu J, Jones IP, Attard GA, Swlenska-Pobell S, Macaskie LE: Microbial synthesis of core/shell gold/palladium nanoparticles for applications in green chemistry. J R Soc Interface 2012, 9: 1705–1712. 10.1098/rsif.2012.0003

    Article  CAS  Google Scholar 

  15. Han GZ, Chen MD: Microwave peak absorption frequency of liquid. S Sci China Ser G-Phys Mech Astron 2008, 51: 1254–1263. 10.1007/s11433-008-0144-0

    Article  CAS  Google Scholar 

  16. Tzima E, Irani-Tehrani M, Kiosses WB, Dejana E, Schultz DA, Engelhardt B, Cao G, DeLisser H, Schwartz MA: A mechanosensory complex that mediates the endothelial cell response to fluid shear stress. Nature 2005, 437: 426–431. 10.1038/nature03952

    Article  CAS  Google Scholar 

  17. Deplanche K, Mikheenko I, Bennett J, Merroun M, Mounzer H, Wood J, Macaskie L: Selective oxidation of benzyl-alcohol over biomass-supported Au/Pd bioinorganic catalysts. Top Catal 2011, 54: 1110–1114. 10.1007/s11244-011-9691-0

    Article  CAS  Google Scholar 

  18. Bunge M, Sobjerg LS, Rotaru AE, Gauthier D, Lindhardt AT, Hause G, Finster K, Kingshott P, Skrydstrup T, Meyer RL: Formation of palladium (0) nanoparticles at microbial surfaces. Biotechnol Bioeng 2010, 107: 206–215. 10.1002/bit.22801

    Article  CAS  Google Scholar 

  19. Wang RF, Wang H, Feng HQ, Ji S: Palladium decorated nickel nanoparticles supported on carbon for formic acid oxidation. Int J Electrochem Sci 2013, 8: 6068–6076.

    CAS  Google Scholar 

  20. Tobin JM, White C, Gadd GM: Metal accumulation by fungi: applications in environmental biotechnology. J Ind Microbiol 1994, 13: 126–130. 10.1007/BF01584110

    Article  CAS  Google Scholar 

  21. Gumbart JC, Beeby M, Jensen GJ, Roux B: Escherichia coli peptidoglycan structure and mechanics as predicted by atomic-scale simulations. Plos Comput Biol 2014, 10: 1003475. 10.1371/journal.pcbi.1003475

    Article  Google Scholar 

  22. Nies DH: Efflux-mediated heavy metal resistance in prokaryotes. FEMS Microbiol Rev 2003, 27: 313–339. 10.1016/S0168-6445(03)00048-2

    Article  CAS  Google Scholar 

  23. Hodgson L, Tarbell JM: Solute transport to the endothelial intercellular cleft: the effect of wall shear stress. Ann Biomedl Eng 2002, 30: 936–945. 10.1114/1.1507846

    Article  Google Scholar 

  24. Silva-Santisteban BOY, Filho FM: Agitation, aeration and shear stress as key factors in inulinase production by Kluyveromyces marxianus . Enzyme Microb Technol 2005, 36: 717–724. 10.1016/j.enzmictec.2004.12.008

    Article  CAS  Google Scholar 

  25. Lantz J, Gardhagen R, Karlsson M: Quantifying turbulent wall shear stress in a subject specific human aorta using large eddy simulation. Med Eng Phys 2012, 34: 1139–1148. 10.1016/j.medengphy.2011.12.002

    Article  Google Scholar 

  26. Allen JJ, Shockling MA, Kunkel GJ, Smits AJ: Turbulent flow in smooth and rough pipes. Phil Trans R Soc A 2007, 365: 699–714. 10.1098/rsta.2006.1939

    Article  CAS  Google Scholar 

  27. Onishi R, Matsuda K, Takahashi K, Kurose R, Komori S: Retrieval of collision kernels from the change of droplet size distributions with linear inversion. Phys Scripta 2008, 2008: 014050. 10.1088/0031-8949/2008/T132/014050

    Article  Google Scholar 

  28. Klaewkla R, Arend M, Hoelderich WF: A review of mass transfer controlling the reaction rate in heterogeneous catalytic systems. In de Mass Transfer-Advanced Aspects. InTech, Germany; 2011:668–684.

    Google Scholar 

  29. Evans JR, Davids WG, MacRae JD, Amirbahman A: Kinetics of cadmium uptake by chitosan-based crab shells. Water Res 2002, 36: 3219–3226. 10.1016/S0043-1354(02)00044-1

    Article  CAS  Google Scholar 

  30. Ghadge RS, Patwardhan AW, Joshi JB: Combined effect of hydrodynamic and interfacial flow parameters on lysozyme deactivation in a stirred tank bioreactor. Biotechnol Prog 2006, 22: 660–672. 10.1021/bp050269s

    Article  CAS  Google Scholar 

  31. Cherry RS, Papoutsakis ET: Hydrodynamic effects on cells in agitated tissue culture reactors. Bioproc Eng 1986, 1: 29–41. 10.1007/BF00369462

    Article  Google Scholar 

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This study was financially supported by the open Project Funding of State Key Laboratory of Bioreactor Engineering of China and the National High Technology Research and Development Program of China (No. 2007AA060904 and No. 2012AA061503).

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Correspondence to Xu Zhang.

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The authors declare that they have no competing interests.

Authors’ contributions

BL, XZ, MLZ, and WST have made substantive intellectual contributions to this study, substantial contributions to the conception and design of it as well as to the acquisition, analysis, and interpretation of data. All of them have been also involved in the drafting and revision of the manuscript. All authors have contributed to and seen the manuscript. All authors read and approved the final manuscript.

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Additional file 1: The supplementary information. The bioreduced of Pd(II) as the function of the shaking speed (Figure S1). The effect of rotating speed with different time to the catalytic activity of BioPd(0) (Figure S2). (DOCX 94 KB)

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Lei, B., Zhang, X., Zhu, M. et al. Effect of fluid shear stress on catalytic activity of biopalladium nanoparticles produced by Klebsiella Pneumoniae ECU-15 on Cr(VI) reduction reaction. Bioresour. Bioprocess. 1, 28 (2014).

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