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Table 2 Contribution of glutamate flow in different cultures

From: Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism

Culture   FB-L FB-H p
Percentage (%) Percentage (%)
Glutamate production Gln-Glu 58.73 ± 3.02 38.98 ± 2.10 **
Asp-Glu 39.26 ± 1.25 59.66 ± 2.34 **
Other 2.01 ± 0.15 1.86 ± 0.03  
Total 100.00 ± 4.42 100.00 ± 4.47  
Other 8.65 ± 0.11 7.66 ± 0.15 **
Glutamate consumption Biomass 1.14 ± 0.03 1.07 ± 0.07  
Antibody 1.14 ± 0.02 1.53 ± 0.09 *
Glu-αKG 55.63 ± 3.03 58.81 ± 3.25  
Glu-Ala 33.44 ± 1.84 31.09 ± 2.16  
Total 100.00 ± 5.03 100.00 ± 6.16  
  1. The p value was calculated between FB-L and FB-H
  2. 0.01 < * p < 0.05, 0.001 < ** p < 0.01, *** p < 0.001