Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism

Bioresources and Bioprocessing

Table 2 Contribution of glutamate flow in different cultures

Culture		FB-L	FB-H	p
Culture		Percentage (%)	Percentage (%)	p
Glutamate production	Gln-Glu	58.73 ± 3.02	38.98 ± 2.10	**
	Asp-Glu	39.26 ± 1.25	59.66 ± 2.34	**
	Other	2.01 ± 0.15	1.86 ± 0.03
	Total	100.00 ± 4.42	100.00 ± 4.47
	Other	8.65 ± 0.11	7.66 ± 0.15	**
Glutamate consumption	Biomass	1.14 ± 0.03	1.07 ± 0.07
	Antibody	1.14 ± 0.02	1.53 ± 0.09	*
	Glu-αKG	55.63 ± 3.03	58.81 ± 3.25
	Glu-Ala	33.44 ± 1.84	31.09 ± 2.16
	Total	100.00 ± 5.03	100.00 ± 6.16