Identification of a new thermostable and alkali-tolerant α-carbonic anhydrase from Lactobacillus delbrueckii as a biocatalyst for CO2 biomineralization

Bioresources and Bioprocessing

Table 1 Comparison of activities among various CAs

Entry	Microorganism or system	Activity (unit)	Half-life at 50 °C (h)	Crystal form of carbonate	Conversion (50 °C/5 min)^a	References
1	P. fragi	70.6 (U/mg)	4	ND	53.3	Sharma et al. (2011)
2	M. lylae	66.5 (U/mg)	4	ND	48.8	Sharma et al. (2011)
3	M. luteus	61.0 (U/mg)	8	ND	44.4	Sharma et al. (2011)
4	INP-N-CA	6.54 × 10⁻² (nU/cell)	ND	Calcite and vaterite	ND	Fan et al. (2011)
5	CA2 surface-displaying yeast cells^b	2.69 × 10⁻¹ (nU/cell)^c	ND	Calcite and vaterite	ND	Barbero et al. (2013)
6	periCA	1.90 (nU/cell)^c	5	Calcite	ND	Jo et al. (2013)
7	LdCA	454.0 (U/mg)	177	Vaterite	91.2	This work

^aConversions were determined by the weight of carbonate quantity after drying
^bEngineering system. This is the name of the gene construct, rather than the name of the recombinant strain
^cnU/cell represents the single-cell activity of each system