Biosynthesis of nanoparticles and silver nanoparticles
© Keat et al. 2015
Received: 23 September 2015
Accepted: 26 November 2015
Published: 14 December 2015
In this century, the development of nanotechnology is projected to be the establishment of a technological evolutionary of this modern era. Recently, nanotechnology is one of the most active subjects of substantial research in modern material sciences and hence metal nanoparticles have a great scientific interest because of their unique optoelectronic and physicochemical properties with applications in diverse areas such as electronics, catalysis, drug delivery, or sensing. Nanotechnology provides an understanding on fundamental properties of objects at the atomic, molecular, and supramolecular levels. Besides, nanotechnology also leads an alternative technological pathway for the exploration and revolution of biological entities, whereas biology provides role models and biosynthetic constituents to nanotechnology. The findings of this review are important to provide an alternative for the green synthesis of silver nanoparticles. It showed more cost-effective and environmental friendly application as well as easier for large production, with relation to the properties of silver nanoparticles as antimicrobial, can be served well as an alternative antiseptic agent in various fields. Typically, silver nanoparticles are smaller than 100 nm and consist of about 20–15,000 silver atoms. Due to the attractive physical and chemical properties of silver at the nanoscale, the development of silver nanoparticles is expanding in recent years and is nowadays significant for consumer and medical products.
In recent years, nanotechnology is an escalating field of modern research (Edhaya Naveena and Prakash 2013) involving in synthesis design, characterization, production, and application of structures, devices, and systems by controlling shape and size at the nanometer scale (Madhuri et al. 2012). Nanotechnology also involves synthesis of nanoparticles of size ranging from 1 to 100 nm (EU 2011; Adlakha-Hutcheon et al. 2009). Moreover, there is a new branch of nanotechnology existing, which is bio-nanotechnology that integrates principles of biology with physical and chemical procedures to generate nano-sized particles with specific functions (Kathiresan et al. 2009; Qi and Wang 2004; Roduner 2006). The bio-based protocols for synthesis of nano-metals are both environmentally and economically green as they are based on green chemistry principles and are simple, relatively inexpensive, and easily scaled up for larger scale production (Mohanpuria et al. 2008; Iravani 2011; Prabhu and Poulose 2012). However, the chemical methods available are often expensive, utilize lethal chemicals, and are comparatively complex. Hence, biosynthesis of nanoparticles using biological agents such as microbes or plant extracts has gained much attention in the area of nanotechnology in last few decades (Malik et al. 2014). Generally, there are three main steps involved in green synthesis method, i.e., reaction medium selection, biological reducing agent selection, and selection of non-carcinogenic substances for stability of nanoparticles (El-Shishtawy et al. 2011). Yet, plant-mediated preparation of nanoparticles can be advantageous over other bio-based synthesis because the procedure of maintaining cell cultures can be omitted and it is also suitable for large scale production under non-aseptic environments (Makarov et al. 2014).
Silver nanoparticles are well-known antimicrobial agents in surgically implanted catheters in order to reduce the infections caused during surgery (Oei et al. 2012) and are proposed to possess anti-fungal, anti-inflammatory, anti-angiogenic, and anti-permeability activities. Silver is also one of the main components in the various creams for healing wounds (Saikia et al. 2015). However, silver nanoparticles are now being introduced as an alternative antibacterial agent replacing silver ions. Both silver ions and silver nanoparticles have inhibitory and lethal effects on bacterial species such as Escherichia coli, Staphylococcus aureus, and even yeast. But, the formation of complexes for silver ions is limited and the effect of the silver ions somehow remains only for a short period (Méndez-Vilas 2011). Yet, this drawback has been resolved by the application of the intact silver nanoparticles which have greater antibacterial properties by promoting the synthesis of reactive oxygen species such as hydrogen peroxide (Mohammed 2015). In addition to antibacterial activity of the silver nanoparticles, a complete disruption of the bacterial membrane of Escherichia coli cells was observed after few minutes in contact with silver nanoparticles under TEM analysis (Raffin et al. 2008). The high efficiency of silver nanoparticles is mainly due to the availability of larger surface area to volume ratio for interactions, easing the penetration and disruption of nanoparticles into the bacterial cells, as compared to micro-sized silver ions (Durán et al. 2010).
Preparation of Nanoparticles
Green synthesis of nanoparticles
There are a variety of chemical and physical preparation methods available for the fabrication of nanoparticles including radiation, chemical precipitation, photochemical methods, electrochemical, and Langmuir–Blodgett techniques, but these methods are often extremely expensive and non-environmental friendly due to the use of toxic, combustible, and hazardous chemicals, which may pose potential environmental and biological risk and high energy requirement (Awwad et al. 2013). The drawbacks of low production rate, structural particle deformation, and inhibition of particle growth are also encountered in these nanoparticles synthesis. Currently, there is a growing need to develop sustainable preparation of nanoparticles that get rid of using harmful organic chemical substances, since noble nanoparticles are widely applied to areas of human contact (Shams et al. 2013). To achieve the principle of green chemistry process, it leads to in search of green synthesis of nanoparticles which can be done by five methods as below:
By using polysaccharide method, metal nanoparticles are synthesized by using water and polysaccharides acting as a reducing agent, a stabilizing agent, or both reducing and stabilizing agents. For example, the fabrication of silver nanoparticles can be performed by using starch as a protective agent and β-d-glucose as a reductant in a mild-heating system. In this way, the attraction between starch and silver nanoparticles is weak and reversible at higher temperatures, facilitating the separation of the synthesized silver nanoparticles (Mochochoko et al. 2013).
The Tollens method is carried out in a one-step process. In this approach, the reduction of Ag+ ions is done by saccharides in the presence of ammonia, yielding silver nanoparticles with different shapes and sizes of 50–200 nm (Korbekandi and Iravani 2012). Ag(NH3) 2 + is a stable complex ion resulting from strong affinity of ammonia for Ag+ ions, so the concentration of ammonia and nature of the reducing agents play a principal role in formulating the AgNP size (Dondi et al. 2012).
Metal nanoparticles can be prepared by using various irradiation methods at room temperature without the use of reducing agent. Hence, temperature-dependent capping agents can also be used in the irradiation method. For instance, silver nanoparticles with a distinct shape and size distribution can be obtained from laser irradiation of an aqueous solution of silver salt and surfactant (Van Phu et al. 2014). Moreover, the morphology of metallic nanoparticles can be controlled by manipulating the radiation dose and dose rate (Abedini et al. 2013).
Through biological method, extracts from biological agents such as microbes and plants can be employed either as reducing or protective agent for the fabrication of metal nanoparticles. In these extracts, various combinations of biomolecules which have the reducing potential can be found such as amino acids, vitamins, proteins, enzymes, and polysaccharides that are environmentally benign, yet chemically complex (Moghaddam 2010). For instance, the unicellular green algae Chlorella vulgaris extract was utilized to synthesize single-crystalline silver nano-plates at room temperature. Proteins in the extract were suggested to perform dual function of Ag+ reduction and shape-control in the synthesis (Annamalai and Nallamuthu 2015).
Polyoxometalates are a vast family of molecular metal-oxide clusters with greater extent of structures. Meanwhile, their reduced forms possess greater capability of electron and proton transfer and/or storage abilities, and thus it can be employed to act as efficient donors or acceptors of several electrons without structural change. Hence, soluble polyoxometalates are capable of synthesizing noble nanoparticles through stepwise, multi-electron redox reactions inertly (Cauerhff and Castro 2013). For examples, a silver salt, Ag2SO4, and polyoxometalates (NH4)10[MoV)4(MoVI)2O14 (O3PCH2PO3)2(HO3PCH2PO3)2]-15 H2O and H7[β-P(MoVI)4(MoVI)8O40] reacted to fabricate spherical and quasi-monodispersed silver nanoparticles with a diameter of about 38 nm after several minutes (Sharma et al. 2009).
In recent years, biological methods employing microbial organisms such as bacteria, actinomycetes, fungi, yeast, viruses, and also plants or plant extracts have gained considerable attention as an alternative to chemical and physical methods in the field of bio-nanotechnology (Khadri et al. 2013). As such, one of the fundamental processes in biosynthesis of nanoparticles involves bio-reduction. Many biological organisms, both unicellular and multicultural, have the ability to produce inorganic materials either intra- or extra- cellular, often of nanoscale dimensions and of exquisite morphology and hierarchical assembly (Pantidos and Horsfall 2014).
Plant-mediated synthesis of nanoparticles
Plant-mediated biosynthesis of nanoparticle is considered a widely acceptable technology for rapid production of metallic nanoparticles for successfully meeting the excessive need and current market demand and resulting in a reduction in the employment or generation of hazardous substances to public health. Similar to microbes which have been used as a “bio-factory” in the synthesis of metallic nanoparticles, plants are also the natural “chemical factories” which are economical and require minimal maintenance (Nyoman Rupiasih et al. 2013).
Plants have several cellular structures and physiological processes to combat the toxicity of metals and maintain homeostasis. They also possess dynamic solutions to detoxify metals and hence scientists have now turned into phytoremediation (Abboud et al. 2013). The modus operandi of detoxification includes immobilization, exclusion, chelation, and compartmentalization of the metals ions, and the expression of more general stress response mechanisms, such as ethylene and stress proteins (Sánchez et al. 2011). The ability to tolerate inimical concentrations of toxic metals is found in the plant kingdom from ages. Their ability to accumulate high concentrations of metals was observed for both essential nutrients, such as copper (Cu), iron (Fe), zinc (Zn), and selenium, as well as non-essential metals, such as cadmium (Cd), mercury (Hg), lead (Pb), aluminum (Al), and arsenic (As) (Sahayaraj et al. 2012). In plants or plant-derived materials, a wide range of metabolites with redox potentials is determined, which are playing a principal role as a reducing agent in the biogenic synthesis of nanoparticles. In comparison to the microbial synthesis of nanoparticles, highly stable nanoparticles are synthesized by plant or plant extracts with the higher rate of production. Consequently, the advantages of plant-mediated preparation of metal nanoparticles lead researchers to in search of further exploration of the bio-reduction mechanism of metal ions by plants and the possible mechanism of formation of metal nanoparticle in and by the plants (Ahmad and Sharma 2012).
Advantages of plant-mediated synthesis of nanoparticles
Due to their easy availability, green preparation of nanoparticles using plant extracts turns out to be an important research subject in the field of bio-nanotechnology in this era. Principally, the biogenic synthesis employs plant extracts in aqueous form in the fabrication of noble nanoparticles for the reason that the availability of reducing agent is higher in the extract than the whole plant (Huang et al. 2007). Besides, plant-mediated synthesis of nanoparticles is simpler and easier to be conducted without requiring any specific operating conditions as compared to typical physical and chemical methods. The synthesized products of the process including waste products are resulted from natural plant extracts, and hence this technique is also more environmental green. Nevertheless, both strong and weak chemical reducing agents and capping agents such as sodium citrate, sodium borohydride, and alcohols, which are mostly toxic, flammable, and cannot be degraded easily are required in the physical and chemical methods (Lalitha et al. 2013).
Through this bio-based protocol of nanoparticles synthesis, higher reproducibility of the process and higher stability of the synthesized nanoparticles can be attained. Therefore, this green-based fabrication of nanoparticles is suitable for large scale production with more effective cost investment, eco-friendly, and safe for human therapeutic use. Apart from the aspects of reproducibility and stability, the rate of bio-reduction of metal ions using biological agents is showed to be much faster and also at ambient temperature and pressure conditions (Pasupuleti et al. 2013). On the contrary, previous studies reported that the bio-reduction potential of the plant extracts is comparatively higher than the microbial culture (Khalil et al. 2014). Moreover, the waste products resulted from the microbial-based method is likely to be more harmful to the environment depending on the type of microbes involved in the synthesis (Moghaddam 2010). Hence, plant-mediated synthesis brings less or almost zero contamination and so reducing the impact on the environment. With all the aforementioned advantages and outstanding features over other methods, the biosynthetic method employing plant extracts has now turned as a simple, effective and viable technique as well as a good alternative to conventional chemical and physical nanoparticle preparation methods, and even microbial methods (Huang et al. 2007).
Silver is a gleaming, very ductile, and malleable element but slightly harder than gold, with a symbol of Ag and atomic number of 47. It is one of the basic elements that make up our planet. In nature, it exists as a native element, as an alloy combining with other metals (e.g., gold) and as minerals (e.g., chlorargyrite and argentite). Chemically, silver possess four different oxidation states, i.e., Ag0, Ag1+, Ag2+, and Ag3+ (Riedel and Kaupp 2009). However, it is a chemically inactive element, but it can be reacted with nitric acid or hot concentrated sulfuric acid, forming soluble silver salts. It also possesses an excellent conductivity of heat and electricity, yet its applications in electrical industry have greatly been limited due to its greater cost (Wang et al. 2013). As for metallic silver form, it is insoluble in water, but its metallic salts such as silver nitrate, AgNO3, and silver chloride, AgCl, are water-soluble. Over past decades, metallic silver is widely applied in surgical prosthesis and splints, coinage, and fungicides (Forough and Farhadi 2010). In contrast, its metallic salts have also been made use of treating various health illness and disorders, e.g., epilepsy, gonorrhea, and gastroenteritis. Due to its good absorptivity, soluble silver compounds have the risk of causing adverse effects on health through dietary intake. Nevertheless, Chen and Schluesener (2008) described that silver is relatively non-toxic and non-carcinogenic to human primary body systems such as nervous, immune, reproductive, or cardiovascular system. Therefore, the demand of silver has been escalating in recent years, exclusively in medical, plastics, and textiles industries.
Uses of various biological entities in the synthesis of silver nanoparticles during the period of 2009–2015
Cycas circinalis, Ficus amplissima, Commelina benghalensis, and Lippia nodiflora
Johnson and Prabu (2015)
Sinapis arvensis seed exudates
Khatami et al. (2015)
Butea monosperma leaf extract
Chaturvedi and Verma (2015)
Musa balbisiana, Azadirachta indica and Ocimum tenuiflorum
Banerjee et al. (2014)
Quercus infectoria extract
Heydari and Rashidipour (2015)
Schizophyllum commune (mushroom fungus)
Arun et al. (2014)
Lantana camara berry
Kumar et al. (2015)
Pomegranate peel extract
Shanmugavadivu et al. (2014)
Aloe vera extract
(SarahIbrahim et al. (2014)
Aegle marmelos (Bael) fruit extract
Nithya Deva Krupa and Raghavan (2014)
14 ± 4
El-Batal et al. (2013)
Sargassum muticum (brown marine macroalgae)
Azizi et al. (2013)
Streptomyces sp. LK3
Karthik et al. (2014)
Glycine max (soybean)
Sasikala et al. (2012)
Schizophyllum radiatum HE863742.1
Metuku et al. (2014)
Tinospora cordifolia leaf extract
Rajathi et al. (2012)
Birla et al. (2013)
Trichoderma reesei (fungus)
Khabat Vahabi et al. (2011)
Saraniya Devi et al. (2013)
Raut Rajesh et al. (2009)
Antimicrobial properties of silver nanoparticles
Bacterial growth and proliferation are adversely inhibited by the adhesion of ultra-small sized silver nanoparticles onto the cell wall of bacteria, resulting in changes in the cell wall which in turn is unable to protect the interior of the cell;
Through the penetration of silver nanoparticles into the bacterial cell, it leads to DNA damage, or even cell death, by altering its normal functioning of bacterial DNA; and
The interaction of Ag+ ions with the proteins containing sulfur present in the bacterial cell wall irreversibly caused the disruption of the bacterial cell wall. This proposed mechanism is also deduced as the main antibacterial mechanism in evaluating the antimicrobial activity.
The antimicrobial effect of silver nanoparticles depends on various parameters including size, shape, and the surface charge of the particles. In this respect, nanoparticles have greater antibacterial properties as they can easily penetrate into the nuclear content of bacteria due to their structure of the bacterial cell wall, especially in gram-negative bacteria, inactivating DNA and the enzymes leading to cellular death. They can also possess a greater surface area for stronger bactericidal interactions (Pal et al. 2007). Furthermore, the antibacterial activity of silver nanoparticles also depends on the morphology of the nanoparticles. Pal et al. (2007) reported that silver nanoparticles with the same surface areas but with different shapes can exhibit dissimilar antibacterial activity which might be due to the difference in their effective surface areas and number of active facets. Truncated triangular silver nano-plates that were found to display the strongest antibacterial activity could be due to their larger surface area to volume ratios and their crystallographic surface structures. In addition, the electrostatic attraction between positively charged nanoparticles and negatively charged bacterial cells is another important factor contributing to the antimicrobial activity of silver nanoparticles. In gram-negative bacteria such as Escherichia coli, Pseudomonas, Salmonella, and Vibrio, its cell wall consists of a layer of lipopolysaccharide at the external surface followed by a thin layer of peptidoglycan. As comparison, the cell wall in gram-positive bacteria such as Bacillus, Clostridium, Staphylococcus, and Streptococcus, is mainly composed of a thick layer of peptidoglycan (Morones et al. 2005). Although gram-positive and gram-negative bacteria have differences in their membrane structures, most of them possess a negative charge on their surfaces. Hence, silver nanoparticles exhibit greater antimicrobial effect against gram-negative bacteria regardless of their resistance level as compared to gram-positive bacteria (Abbaszadegan et al. 2015).
Application of silver nanoparticles
Due to the significant antimicrobial properties of AgNPs against wide ranging microorganisms, numerous medical applications impregnated with AgNPs such as catheters and cardiovascular and bone implants have been recognized for hindering the formation of biofilm and lowering the risk of pathogenic invasion (Tran et al. 2013). Typically, ultrahigh molecular weight polyethylene has been widely used as an insert for artificial joint replacement, but its application is somehow limited due to its high susceptibility to wear and tear (Morley et al. 2007). Yet, the drawback of wear and tear of the polymer is significantly abridged by the addition of silver nanoparticles. In addition, with the excellent antibacterial properties of silver nanoparticles, it is also loaded with polymethyl methacrylate broadly as bone cements that are broadly used as synthetic joint replacement (Alt et al. 2004). In 2010, Xing et al. deduced that (poly-(-3-hydroxybutyrate-co-3-hydroxyvalerate) PHBV nanofiber scaffolds containing AgNPs have the tendency of aiding in bone and skin tissue regeneration from their extensive study on both osteoblast (bone cells) and fibroblast (skin cells) cultured on such scaffolds. Hence, the risk associated with implantation surgery can be overcome by fabricating the surface of structure of the bone implants devices and scaffolds with silver nanoparticles (Xing et al. 2010).
Furthermore, silver nanoparticles are also applied in nano-crystalline dressings for the therapy of wound or hospital-acquired infections, minimizing the inflammatory response (Fong and Wood 2006). For instance, the classical surgical meshes are used to bridge severe wounds and for tissue therapy, but it is highly vulnerable to pathogenic invasions. Thus, the effectiveness of these meshes is enhanced with the impregnation with silver nanoparticles. With the plasmonic properties of silver nanoparticles, it can be widely applied in bio-imaging for monitoring dynamic events over an extended period of time without undergoing photo-bleaching as compared to common fluorescent dyes. Therefore, the conjugation of cells to the target cells leads to the conversion of light energy to thermal energy and then resulted in thermal ablation of the target cells, aiding in destroying unwanted or damaged cells (Loo et al. 2005). In addition, the plasmonic properties of silver nanoparticles can be exploited for bio-sensing, which can effectively detect wide ranging of proteins that typical biosensors do not. With this unique capability, silver nanoparticles are broadly employed for detecting various abnormalities and diseases in human body system, e.g., tumor cells or cancer. The plasmonic properties of silver nanoparticles are somehow dependent on its size, shape, and the dielectric potential of surrounding medium (Morley et al. 2007).
In recent years, silver nanoparticles are widely applied in chemical industry as an additive to cosmetics, because silver nanoparticles satisfy the requirements of excellent antiseptic properties, as a safe preservative additive, and also as a constituent for the skin therapy, e.g., treatment of acne (Kokura et al. 2010). In addition to the applications of silver nanoparticles in medical and environmental protection field, silver nanoparticle-coated paper could also serve a critical role in food preservation in which provides a reservoir for slow releasing of ionic silver from the surface to the bulk to prevent microbial growth in the food as well as to prevent growth of pathogens on the surface itself (Gottesman et al. 2011). Owing to the excellent antimicrobial activity of silver nanoparticles, developing antibacterial coatings on surfaces has drawn much interest for human health and environmental protection in the paint coating industry. In 2008, John et al. demonstrated green synthesis techniques of metallic nanoparticle-embedded paints using common household paint in a single step. Through the naturally occurring oxidative drying process in oils that involves free-radical exchange, reduction of metal salts and dispersion of metal nanoparticles in the oil media were successfully done without the use of any external chemical reducing or stabilizing agents. The resulting well-dispersed metal nanoparticles in oil dispersions can then be directly used on different surfaces such as wood, glass, steel, and different polymer surfaces, and also exhibit excellent bactericidal properties against gram-positive and gram-negative bacteria, especially silver nanoparticle-embedded paints (Kumar et al. 2008).
Toxicity of silver nanoparticles
Generally, silver nanoparticles can be considered as an ideal candidate for numerous applications in various fields, especially in biomedical industry as in diagnosis, drug delivery, cell imaging, and implantation, even so several studies reported that silver nanoparticles possess an adverse effect on humans as well as the environment. In one of the toxicology researches of silver nanoparticles, in vitro toxicity assay in rat liver cells has conducted and demonstrated that silver nanoparticles caused oxidative stress and cease of mitochondrial function even at low level of exposure to silver nanoparticles (10–50 μg ml−1). Yet, at higher doses (>1.0 mg L−1), AgNPs exhibited a significant cytotoxicity and caused abnormal cellular morphology, cellular shrinkage, and acquisition of an irregular shape (McAuliffe and Perry 2007). Besides, silver nanoparticles can also induce toxicity to in vitro mouse germ line stem cells by impairing mitochondrial activities and cause leakage through the cell membranes by changing its permeability to sodium and potassium ions. Therefore, the cytotoxic mechanism of AgNPs is predominantly based on the induction of reactive oxygen species (ROS). Specifically, exposure to silver nanoparticles triggers depletion in glutathione level, elevation of ROS levels, lipid peroxidation, and increased expression of ROS responsive genes, leading to DNA damage, apoptosis, and necrosis (Haider and Kang 2015).
As a result of large surface area to volume ratio, human bodies are vulnerably exposed to silver nanoparticles via ingestion, inhalation, or skin, and its penetrating potential is greatly increased, and hence it has the capability of penetrating into the circulatory system and even translocating boundlessly in the human body system (Sung et al. 2008). Hence, previous research elaborated that silver nanoparticles can allegedly induce toxicity to the male reproductive system by crossing through blood-testes barrier and depositing in the testes. Moreover, silver nanoparticles can induce adversely toxic effect on the proliferation and cytokine expression by peripheral blood mononuclear cells (McAuliffe and Perry 2007). Alternatively, Kim et al. evaluated that silver nanoparticles do not induce genetic toxicity in male and female Sprague–Dawley rat bone marrow in vivo in his gastrointestinal toxicology study. In his experiment, there were no significant changes in body weight of male and female rats relative to the doses of AgNPs (size of 60 nm) over a period of 28 days. However, alkaline phosphatase and cholesterol values were found to be altered with the exposure to over more than 300 mg of AgNPs, resulting in minor liver damage (Kim et al. 2008).
Though aforementioned studies tend to suggest that silver nanoparticles can adversely induce toxicity to living beings, relatively less in vivo toxicology researches of silver nanoparticles were established which are drastically different from in vitro condition. Thus, further investigation is required to assess the toxicity effect of silver nanoparticles in in vivo condition for evaluating its exact toxicity to human and animals.
Chemical and physical syntheses of nanoparticles are unable to be expanded easily to large scale production due to several drawbacks such as the presence of toxic organic solvents, production of hazardous by-products and intermediate compounds, and high energy consumption. This could lead to an increase in the particle reactivity and toxicity, which might harm human health and environment due to the composition ambiguity and lack of predictability. Therefore, this leads to biological methods which could be more eco-friendly and does not cause any harm to human and domestic animals health.
NAE was responsible for the manuscript approval and final approval. AME was responsible for manuscript writing; AA and CLK were responsible for manuscript drafting. All authors read and approved the final manuscript.
The authors of this manuscript do not have any financial competing interests to declare.
Open AccessThis article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.
- Abbaszadegan A, Ghahramani Y, Gholami A, Hemmateenejad B, Dorostkar S, Nabavizadeh M, Sharghi H (2015) The effect of charge at the surface of silver nanoparticles on antimicrobial activity against gram-positive and gram-negative bacteria: a preliminary study. J NanomatGoogle Scholar
- Abboud Y, Eddahbi A, El Bouari A, Aitenneite H, Brouzi K, Mouslim J (2013) Microwave-assisted approach for rapid and green phytosynthesis of silver nanoparticles using aqueous onion (Allium cepa) extract and their antibacterial activity. J Nanostruct Chem 3(1):1–7. doi:10.1186/2193-8865-3-84 View ArticleGoogle Scholar
- Abedini A, Daud AR, Hamid MAA, Othman NK, Saion E (2013) A review on radiation-induced nucleation and growth of colloidal metallic nanoparticles. Nanoscale Res Lett 8(1):1–10View ArticleGoogle Scholar
- Abou El-Nour KMM, Aa Eftaiha, Al-Warthan A, Ammar RAA (2010) Synthesis and applications of silver nanoparticles. Arab J Chem 3(3):135–140. doi:10.1016/j.arabjc.2010.04.008 View ArticleGoogle Scholar
- Adlakha-Hutcheon G, Khaydarov R, Korenstein R, Varma R, Vaseashta A, Stamm H, Abdel-Mottaleb M (2009) Nanomaterials, nanotechnology. In: Linkov I, Steevens J (eds) Nanomaterials: Risks and Benefits. NATO Science for Peace and Security Series C: Environmental Security. Springer, Netherlands. pp 195–207. doi:10.1007/978-1-4020-9491-0_14
- Ahmad Naheed, Sharma S (2012) Green synthesis of silver nanoparticles using extracts of Ananas comosus. Green Suistain Chem 2:141–147View ArticleGoogle Scholar
- Alt V, Bechert T, Steinrücke P, Wagener M, Seidel P, Dingeldein E, Domann E, Schnettler R (2004) An in vitro assessment of the antibacterial properties and cytotoxicity of nanoparticulate silver bone cement. Biomaterials 25(18):4383–4391View ArticleGoogle Scholar
- Annamalai J, Nallamuthu T (2015) Green synthesis of silver nanoparticles: characterization and determination of antibacterial potency. Appl Nanosci 1–7Google Scholar
- Arun G, Eyini M, Gunasekaran P (2014) Green synthesis of silver nanoparticles using the mushroom fungus Schizophyllum commune and its biomedical applications. Biotechnol Bioprocess Eng 19(6):1083–1090. doi:10.1007/s12257-014-0071-z View ArticleGoogle Scholar
- Awwad A, Salem N, Abdeen A (2013) Green synthesis of silver nanoparticles using carob leaf extract and its antibacterial activity. Int J Ind Chem 4(1):1–6. doi:10.1186/2228-5547-4-29 View ArticleGoogle Scholar
- Azizi S, Namvar F, Mahdavi M, Ahmad MB, Mohamad R (2013) Biosynthesis of silver nanoparticles using brown marine macroalga, Sargassum muticum aqueous extract. Materials. doi:10.3390/ma6125942 Google Scholar
- Banerjee P, Satapathy M, Mukhopahayay A, Das P (2014) Leaf extract mediated green synthesis of silver nanoparticles from widely available Indian plants: synthesis, characterization, antimicrobial property and toxicity analysis. Bioresour Bioprocess 1(1):3View ArticleGoogle Scholar
- Birla SS, Gaikwad SC, Gade AK, Rai MK (2013) Rapid synthesis of silver nanoparticles from Fusarium oxysporum by optimizing physicocultural conditions. Scient World J 2013:12. doi:10.1155/2013/796018 View ArticleGoogle Scholar
- Cauerhff Ana, Castro GR (2013) Bionanoparticles, a green nanochemistry approach. Electron J Biotechnol. doi:10.2225/vol16-issue3-fulltext-3 Google Scholar
- Chaturvedi V, Verma P (2015) Fabrication of silver nanoparticles from leaf extract of Butea monosperma (Flame of Forest) and their inhibitory effect on bloom-forming cyanobacteria. Bioresour Bioprocess 2(1):18View ArticleGoogle Scholar
- Chen X, Schluesener HJ (2008) Nanosilver: a nanoproduct in medical application. Toxicol Lett 176(1):1–12. doi:10.1016/j.toxlet.2007.10.004 View ArticleGoogle Scholar
- Dondi R, Su W, Griffith GA, Clark G, Burley GA (2012) Highly size-and shape-controlled synthesis of silver nanoparticles via a templated tollens reaction. Small 8(5):770–776View ArticleGoogle Scholar
- Durán Nelson, Marcato Priscyla D, De Conti Roseli, Alves Oswaldo L, Costab FTM, Brocchib M (2010) Potential use of silver nanoparticles on pathogenic bacteria, their toxicity and possible mechanisms of action. J Braz Chem Soc 21(6):949–959View ArticleGoogle Scholar
- Edhaya Naveena B, Prakash S (2013) Biological synthesis of gold nanoparticles using marine algae Gracilaria corticata and its application as a potent antimicrobial and antioxidant agent. Asian J Pharm Clin Res 6(2):179–182Google Scholar
- El-Batal AI, Amin MA, Shehata MM, Hallol MM (2013) Synthesis of silver nanoparticles by Bacillus stearothermophillus using gamma radiation and their antimicrobial activity. World Appl Sci J 22(1):01–16. doi:10.5829Google Scholar
- El-Kheshen Amany A, El-Rab SFG (2012) Effect of reducing and protecting agents on size of silver nanoparticles and their anti-bacterial activity. Schol Res Librar 4(1):53–65Google Scholar
- El-Shishtawy RM, Asiri AM, Al-Otaibi MM (2011) Synthesis and spectroscopic studies of stable aqueous dispersion of silver nanoparticles. Spectrochim Acta Part A Mol Biomol Spectrosc 79(5):1505–1510. doi:10.1016/j.saa.2011.05.007 View ArticleGoogle Scholar
- EU (2011) Commission Recommendation of 18 October 2011 on the definition of nanomaterial (2011/696/EU). Official Journal of the European Union 2011 L275/38Google Scholar
- Fong J, Wood F (2006) Nanocrystalline silver dressings in wound management: a review. Int J Nanomed 1(4):441–449View ArticleGoogle Scholar
- Forough Mehrdad, Farhadi K (2010) Biological and green synthesis of silver nanoparticles. Turkish J Eng Env Sci 34:281–287. doi:10.3906/muh-1005-30 Google Scholar
- Gottesman R, Shukla S, Perkas N, Solovyov LA, Nitzan Y, Gedanken A (2011) Sonochemical coating of paper by microbiocidal silver nanoparticles. Langmuir 27(2):720–726. doi:10.1021/la103401z View ArticleGoogle Scholar
- Guo J-Z, Cui H, Zhou W, Wang W (2008) Ag nanoparticle-catalyzed chemiluminescene reaction between luminol and hydrogen peroxide. J Photochem Photobiol A 193(2–3):89–96View ArticleGoogle Scholar
- Haider A, Kang I-K (2015) Preparation of silver nanoparticles and their industrial and biomedical applications: a comprehensive review. Adv Mat Sci EngGoogle Scholar
- Heydari R, Rashidipour M (2015) Green synthesis of silver nanoparticles using extract of oak fruit hull (Jaft): synthesis and in vitro cytotoxic effect on MCF-7 cells. Int J Breast Cancer 2015:6. doi:10.1155/2015/846743 View ArticleGoogle Scholar
- Huang Jiale, Qingbiao L, Sun Daohua, Yinghua Lu, Yuanbo Su, Yang Xin, Wang Huixuan, Wang Yuaneng, Shao Wenyao, He Ning, Hong Jinqing, Chen C (2007) Biosynthesis of silver and gold nanoparticles by novel sundried Cinnamomum camphora leaf. Nanotechnology. doi:10.1088/0957-4484/18/10/105104 Google Scholar
- Iravani S (2011) Green synthesis of metal nanoparticles using plants. Green Chem 13(10):2638–2650. doi:10.1039/C1GC15386B View ArticleGoogle Scholar
- Johnson I, Prabu HJ (2015) Green synthesis and characterization of silver nanoparticles by leaf extracts of Cycas circinalis, Ficus amplissima, Commelina benghalensis and Lippia nodiflora. Int Nano Lett 5(1):43–51. doi:10.1007/s40089-014-0136-1 View ArticleGoogle Scholar
- Karthik L, Kumar G, Kirthi AV, Rahuman AA, Bhaskara Rao KV (2014) Streptomyces sp. LK3 mediated synthesis of silver nanoparticles and its biomedical application. Bioprocess Biosyst Eng 37(2):261–267. doi:10.1007/s00449-013-0994-3 View ArticleGoogle Scholar
- Kathiresan K, Manivannan S, Nabeel MA, Dhivya B (2009) Studies on silver nanoparticles synthesized by a marine fungus, Penicillium fellutanum isolated from coastal mangrove sediment. Colloids Surf B 71(1):133–137. doi:10.1016/j.colsurfb.2009.01.016 View ArticleGoogle Scholar
- Khabat Vahabi G, Mansoori Ali, Karimi S (2011) Biosynthesis of silver nanoparticles by fungus Trichoderma Reesei. Insci J 1(1):65–79. doi:10.5640/insc.010165 View ArticleGoogle Scholar
- Khadri Habeeb, Alzohariy Mohammad, Janardhan Avilala, Kumar Arthala Praveen, Narasimha G (2013) Green synthesis of silver nanoparticles with high fungicidal activity from olive seed extract. Adv Nanopart 2:241–246View ArticleGoogle Scholar
- Khalil MMH, Ismail EH, El-Baghdady KZ, Mohamed D (2014) Green synthesis of silver nanoparticles using olive leaf extract and its antibacterial activity. Arab J Chem 7(6):1131–1139. doi:10.1016/j.arabjc.2013.04.007 View ArticleGoogle Scholar
- Khatami M, Pourseyedi S, Khatami M, Hamidi H, Zaeifi M, Soltani L (2015) Synthesis of silver nanoparticles using seed exudates of Sinapis arvensis as a novel bioresource, and evaluation of their antifungal activity. Bioresour Bioprocess 2(1):19View ArticleGoogle Scholar
- Kim YS, Kim JS, Cho HS, Rha DS, Kim JM, Park JD, Choi BS, Lim R, Chang HK, Chung YH (2008) Twenty-eight-day oral toxicity, genotoxicity, and gender-related tissue distribution of silver nanoparticles in Sprague-Dawley rats. Inhalation Toxicol 20(6):575–583View ArticleGoogle Scholar
- Kokura Satoshi, Handa Osamu, Takagi Tomohisa, Ishikawa Takeshi, Naito Yuji, Yoshikawa T (2010) Silver nanoparticles as a safe preservative for use in cosmetics. Nanomedicine: nanotechnology. Biol Med 6:570–574. doi:10.1016/j.nano.2009.12.002 Google Scholar
- Korbekandi H, Iravani S (2012) Silver Nanoparticles. INTECH Open Access PublisherGoogle Scholar
- Kumar A, Vemula PK, Ajayan PM, John G (2008) Silver-nanoparticle-embedded antimicrobial paints based on vegetable oil. Nat Mater 7(3):236–241. doi:10.1038/nmat2099 View ArticleGoogle Scholar
- Kumar B, Kumari S, Cumbal L, Debut A (2015) Lantana camara berry for the synthesis of silver nanoparticles. Asian Pacific J Trop Biomed 5(3):192–195. doi:10.1016/S2221-1691(15)30005-8 View ArticleGoogle Scholar
- Lalitha A, Subbaiya R, Ponmurugan P (2013) Green synthesis of silver nanoparticles from leaf extract Azhadirachta indica and to study its anti-bacterial and antioxidant property. Int Curr Microbiol Appl Sci 2(6):228–235Google Scholar
- Li Q, Mahendra S, Lyon DY, Brunet L, Liga MV, Li D, Alvarez PJ (2008) Antimicrobial nanomaterials for water disinfection and microbial control: potential applications and implications. Water Res 42(18):4591–4602View ArticleGoogle Scholar
- Loo C, Lowery A, Halas N, West J, Drezek R (2005) Immuno targeted nanoshells for integrated cancer imaging and therapy. Nano Lett 5(4):709–711View ArticleGoogle Scholar
- Madhuri S, Maheshwar S, Sunil P, Oza G (2012) Nanotechnology: concepts and applications, vol 4. CRC Press, USAGoogle Scholar
- Makarov VV, Love AJ, Sinitsyna OV, Makarova SS, Yaminsky IV, Taliansky ME, Kalinina NO (2014) “Green” nanotechnologies: synthesis of metal nanoparticles using plants. Acta Naturae 6(1):35–44Google Scholar
- Malik P, Shankar R, Malik V, Sharma N, Mukherjee TK (2014) Green chemistry based benign routes for nanoparticle synthesis. J Nanopar 2014:14. doi:10.1155/2014/302429 View ArticleGoogle Scholar
- Mason Cynthis, Vivekanadhan Singaravelu, Misra Manjusri, Mohanty AK (2012) Switchgrass (Panicum virgatum) extract mediated green synthesis of silver nanoparticles. World J Nano Sci Eng 2:47–52View ArticleGoogle Scholar
- McAuliffe ME, Perry MJ (2007) Are nanoparticles potential male reproductive toxicants? A literature review. Nanotoxicology 1(3):204–210View ArticleGoogle Scholar
- Méndez-Vilas A (2011) Science Against Microbial Pathogens: Communicating Current Research and Technological Advances. vol v. 2. Formatex Research CenterGoogle Scholar
- Merga G, Wilson R, Lynn G, Milosavljevic BH, Meisel D (2007) Redox catalysis on “Naked” silver nanoparticles. J Phys Chem C 111(33):12220–12226. doi:10.1021/jp074257w View ArticleGoogle Scholar
- Metuku R, Pabba S, Burra S, Hima Bindu N SVSSSL, Gudikandula K, Singara Charya MA (2014) Biosynthesis of silver nanoparticles from Schizophyllum radiatum HE 863742.1: their characterization and antimicrobial activity. Biotech 4(3):227–234. doi:10.1007/s13205-013-0138-0
- Mochochoko T, Oluwafemi OS, Jumbam DN, Songca SP (2013) Green synthesis of silver nanoparticles using cellulose extracted from an aquatic weed; water hyacinth. Carbohydr Polym 98(1):290–294. doi:10.1016/j.carbpol.2013.05.038 View ArticleGoogle Scholar
- Moghaddam KM (2010) An Introduction to microbial metal nanoparticle preparation method. J Young Invest 19(19):1–6Google Scholar
- Mohammed AE (2015) Green synthesis, antimicrobial and cytotoxic effects of silver nanoparticles mediated by Eucalyptus camaldulensis leaf extract. Asian Pacific J Trop Biomed 5(5):382–386. doi:10.1016/S2221-1691(15)30373-7 View ArticleGoogle Scholar
- Mohanpuria P, Rana N, Yadav S (2008) Biosynthesis of nanoparticles: technological concepts and future applications. J Nanopart Res 10(3):507–517. doi:10.1007/s11051-007-9275-x View ArticleGoogle Scholar
- Morley K, Webb P, Tokareva N, Krasnov A, Popov V, Zhang J, Roberts C, Howdle S (2007) Synthesis and characterisation of advanced UHMWPE/silver nanocomposites for biomedical applications. Eur Polymer J 43(2):307–314View ArticleGoogle Scholar
- Morones JR, Elechiguerra JL, Camacho A, Holt K, Kouri JB, Ramírez JT, Yacaman MJ (2005) The bactericidal effect of silver nanoparticles. Nanotechnology 16(10):2346View ArticleGoogle Scholar
- Nithya Deva Krupa A, Raghavan V (2014) Biosynthesis of silver nanoparticles using Aegle marmelos (Bael) fruit extract and its application to prevent adhesion of bacteria: a strategy to control microfouling. Bioinorg Chem Appl 2014:8. doi:10.1155/2014/949538 Google Scholar
- Nyoman Rupiasih N, Aher Avinash, Gosavi Suresh, Vidyasagar PB (2013) Green synthesis of silver nanoparticles using latex extract of Thevetia peruviana: a novel approach towards poisonous plant utilization. J Phys Conf Series. doi:10.1088/1742-6596/423/1/012032 Google Scholar
- Oei JD, Zhao WW, Chu L, DeSilva MN, Ghimire A, Rawls HR, Whang K (2012) Antimicrobial acrylic materials with in situ generated silver nanoparticles. J Biomed Mater Res B Appl Biomater 100(2):409–415. doi:10.1002/jbm.b.31963 View ArticleGoogle Scholar
- Pal S, Tak YK, Song JM (2007) Does the antibacterial activity of silver nanoparticles depend on the shape of the nanoparticle? A study of the gram-negative bacterium Escherichia coli. Appl Environ Microbiol 73(6):1712–1720View ArticleGoogle Scholar
- Pal Sovan Lal, Utpal Jana PK, Manna GP, Mohanta Manavalan R (2011) Nanoparticle: an overview of preparation and characterization. J Appl Pharma Sci 1(6):228–234Google Scholar
- Pantidos Nikolaos, Horsfall LE (2014) Biological synthesis of metallic nanoparticles by bacteria, fungi and plants. Nanomed Nanotechnol. doi:10.4172/2157-7439.1000233 Google Scholar
- Pasupuleti VR, Prasad T, Shiekh RA, Balam SK, Narasimhulu G, Reddy CS, Rahman IA, Gan SH (2013) Biogenic silver nanoparticles using Rhinacanthus nasutus leaf extract: synthesis, spectral analysis, and antimicrobial studies. Int J Nanomed 8:3355–3364. doi:10.2147/IJN.S49000 View ArticleGoogle Scholar
- Prabhu S, Poulose E (2012) Silver nanoparticles: mechanism of antimicrobial action, synthesis, medical applications, and toxicity effects. Int Nano Lett 2(1):1–10. doi:10.1186/2228-5326-2-32 View ArticleGoogle Scholar
- Qi WH, Wang MP (2004) Size and shape dependent melting temperature of metallic nanoparticles. Mater Chem Phys 88:280–284. doi:10.1016/j.matchemphys.2004.04.026 View ArticleGoogle Scholar
- Raffin M, Hussain F, Bhatti TM, Akhter JI, Hameed A, Hasan MM (2008) J Mat Sci Technol 24:192–196Google Scholar
- Rajathi K, Vijaya Raj D, Anarkali J, Sridhar S (2012) Green Synthesis, characterization and in-vitro antibacterial activity of silver nanoparticles by using Tinospora cordifolia leaf extract. Int J Green Chem Bioprocess 2(2):15–19Google Scholar
- Raut Rajesh W, Lakkakula Jaya R, Kolekar Niranjan S, Mendhulkar Vijay D, Kashid Sahebrao B (2009) Photosynthesis of Silver Nanoparticle Using Gliricidia sepium (Jacq.). Current Nanoscience 5(1):117–122Google Scholar
- Riedel Sebastian, Kaupp Martin (2009) The highest oxidation states of the transition metal elemtns. Coord Chem Rev 253(5–6):606–624. doi:10.1016/j.ccr.2008.07.014 View ArticleGoogle Scholar
- Roduner E (2006) Size matters: why nanomaterials are different. Chem Soc Rev 35(7):583–592. doi:10.1039/B502142C View ArticleGoogle Scholar
- Sahayaraj K, Rajesh S, Rathi JM (2012) Silver nanoparticles biosynthesis using marine alga Padina pavonica (Linn.) and its microbicidal activity. Digest J Nanomat Biostruct 7(4):1557–1567Google Scholar
- Saikia Dulen, Gogoi Pradip K, Phukan Pallabi, Bhuyan Nilave, Borchetia Sangeeta, Saika J (2015) Green synthesis of silver nanoparticles using Asiatic Pennywort and Bryophyllum leaves extract and their antimicrobial activity. Adv Mat Lett 6(3):260–264. doi:10.518/amlett.2015.5655 Google Scholar
- Sánchez Elpidio Morales-, Guahardo-Pacheco Jesús, Noriega-Treviño Maria, Quintero-González Cristina, Compeán-Jasso Martha, López-Salinas Francisco, González-Hernández Jesús, Ruiz F (2011) Synthesis of silver nanoparticles using albumin as a reducing agent. Mat Sci Appl 2:578–581. doi:10.4236/msa.2011.26077 Google Scholar
- SarahIbrahim H, Ayad M, Fadhil A, Nk Al-Ani (2014) Production of Ag nanoparticles using Aloe vera extract and its antimicrobial activity. J Al-Nahrain Univ 17(2):165–171Google Scholar
- Saraniya Devi J, Valentin Bhimba B, Peter DM (2013) Production of biogenic Silver nanoparticles using Sargassum longifolium and its application. Indian J Geo-Marine Sci 42(7):125–130Google Scholar
- Sasikala D, Govindaraju K, Tamilselvan S, Singaravelu G (2012) Soybean protein: a natural source for the production of green silver nanoparticles. Biotechnol Bioprocess Eng 17(6):1176–1181. doi:10.1007/s12257-012-0021-6 View ArticleGoogle Scholar
- Shams Somaye, Pourseyedi Shahram, Raisi M (2013) Green Synthesis of Ag nanoparticles in the present of Lens culinaris seed exudates. Int J Agrcul Crop Sci 5(23):2812–2815Google Scholar
- Shanmugavadivu M, Selvam K, Ranjithkumar R (2014) Synthesis of pomegranate peel extract mediated silver nanoparticles and its antibacterial activity. Am J Adv Drug Deliv 2(2)Google Scholar
- Sharma VK, Yngard RA, Lin Y (2009) Silver nanoparticles: green synthesis and their antimicrobial activities. Adv Colloid Interface Sci 145(1):83–96View ArticleGoogle Scholar
- Sung JH, Ji JH, Yoon JU, Kim DS, Song MY, Jeong J, Han BS, Han JH, Chung YH, Kim J (2008) Lung function changes in Sprague-Dawley rats after prolonged inhalation exposure to silver nanoparticles. Inhalation Toxicol 20(6):567–574View ArticleGoogle Scholar
- Tran Quang Huy, Nguyenm VQ, Le A-T (2013) Silver nanoparticles: synthesis, properties, toxicology, applications and perspectives. Adv Nat Sci Nanosci Nanotechnol. doi:10.1088/2043-6262-4-3-033001 Google Scholar
- Van Phu D, le Quoc A, Duy NN, Lan NT, Du BD, le Luan Q, Hien NQ (2014) Study on antibacterial activity of silver nanoparticles synthesized by gamma irradiation method using different stabilizers. Nanoscale Res Lett 9(1):162. doi:10.1186/1556-276x-9-162 View ArticleGoogle Scholar
- Wang MY, Shen T, Wang M, Zhang D, Chen J (2013) One-pot green synthesis of Ag nanoparticles-decorated reduced graphene oxide for efficient nonenzymatic H2O2 biosensor. Mater Lett 107:311–314. doi:10.1016/j.matlet.2013.06.031 View ArticleGoogle Scholar
- Xing Z-C, Chae W-P, Baek J-Y, Choi M-J, Jung Y, Kang I-K (2010) In vitro assessment of antibacterial activity and cytocompatibility of silver-containing PHBV nanofibrous scaffolds for tissue engineering. Biomacromolecules 11(5):1248–1253View ArticleGoogle Scholar