Heterologous expression of an acidophilic multicopper oxidase in Escherichia coli and its applications in biorecovery of gold

Bioresources and Bioprocessing

Table 2 Comparison of the catalytic kinetic parameters of MCO on ABTS from different microorganisms

Sources	Gene	V_max μmol/min	k _cat 1/min	K _m mM	k _cat /K _m 1/mM^a/min	Reaction condition	Reference
Bacillus tequilensis SN4	SN4LAC	ND	4020	1.4	2871	pH 5.5 at 85 °C	Sondhi et al. (2014)
Mangrove soil	Lac591^a	ND	2.52	0.09	28.0	pH 7.4 at 55 °C	Ye et al. (2010)
Thioalkalivibrio sp. ALRh	lacc	1500	129	4.6	28.0	pH 5 at 50 °C	Ausec et al. (2015)
Pseudomonas sp. 593	cumA	ND	23.58	0.1	235.8	pH 5 at 55 °C	Yang et al. (2016)
Bacillus sp. SL-1	cotA	ND	1380	0.046	30,000	pH 4.5 at 50 °C	Safary et al. (2016)
Proteus hauseri	LacB^a	58	1328	0.303	4382	pH 2.2 at 50 °C	This study
Proteus hauseri	LacB-Au^b	5.59	52.7	0.129	408.5	pH 2.2 at 50 °C	This study

^aThe yields of LacB of Proteus hauseri and Lac591 of Mangrove soil expressed in E. coli are 1330 and 1381 mg/L, respectively
^bLacB-Au means with gold binding and followed by activity analysis