Skip to main content
Bioresources and Bioprocessing
Download PDF

Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing

Bioresources and Bioprocessing volume 4, Article number: 40 (2017) Cite this article

Abstract

Objectives

This report explores the possibility of synthesizing enzyme–metal–organic framework (MOF) composites by ink-jet printing.

Results

This study demonstrates that the direct synthesis of patterned enzyme–metal–organic framework (MOF) composites on various substrates including paper and polymeric films can be readily achieved by ink-jet printing bio-inks containing protein molecules, metal ions, and organic ligands loaded, respectively, in different cartridges. The formed Cytochrome c (Cyt c)–MOF composites on filter paper by ink-jet printing can be used for rapid detection of hydrogen peroxide in solution.

Conclusions

This technique opens possibilities of scalable, controllable, and designable fabrication of functional protein–MOF hybrid surface with promising applications in future bio-related application fields such as biosensing, wearable bioelectronics, artificial biomimetic membranes, and tissue engineering.

A direct synthesis of enzyme–MOF composites can be readily achieved by a ink-jet printing process

Background

As a type of designable porous materials with numerous structural possibilities, metal–organic frameworks (MOFs) have been witnessed with their great progress in recent years. The focus of MOF applications is gas storage, separation, sensing, and catalysis (Furukawa et al. 2013; Hayashi et al. 2007; Sumida et al. 2011; Zhou et al. 2012). Moreover, MOFs also have shown great potentials in hosting biomolecules such as enzyme (or protein), explored by pioneering work from several groups in recent years (Lykourinou et al. 2011; Chen et al. 2012; Deng et al. 2012; Lyu et al. 2014; Shieh et al. 2015; Liang et al. 2015a; Feng et al. 2015; Li et al. 2016a, b, c; Lian et al. 2016, 2017; Liao et al. 2017; Zhang et al. 2017; Wu et al. 2015a, b; Hou and Ge 2017). The rigid but porous structure of MOFs makes them ideal for immobilizing flexible protein molecules to increase the overall structural stability of protein. Benefited from the protection effect of MOF scaffolds, the incorporated enzyme can resist to various harsh environments that are harmful to fragile native enzyme, (Shieh et al. 2015; Liang et al. 2015a; Feng et al. 2015) even at protein-denaturing conditions (Liao et al. 2017; Wu et al. 2017). The enhanced stability makes enzyme–MOF composites promising for industrial and biomedical applications such as biocatalysis at harsh conditions (Lykourinou et al. 2011; Feng et al. 2015; He et al. 2016), enzymatic degradation of nerve agents, (Li et al. 2016a, b) stabilizing tobacco mosaic virus (Lian et al. 2016), and increasing sensitivity of biosensors (Lyu et al. 2014; Zhang et al. 2017). In addition, the tailor-made pore structures of MOFs realized the fitting of multiple enzymes in position for highly efficient enzyme cascade (Wu et al. 2015a, b; Lian et al. 2016).

As exemplified by the above studies, two strategies of synthesizing enzyme–MOF composites have been developed. One is the post-immobilization strategy. For example, by incubating enzyme (or protein) molecules with pre-synthesized MOFs in aqueous solution, enzyme (or protein) molecules were adsorbed into the large pores of MOFs (Lykourinou et al. 2011; Chen et al. 2012; Deng et al. 2012; Feng et al. 2015; Li et al. 2016a, b; Zhang et al. 2017; Lian et al. 2016). By chemical reactions between amino acids of enzyme and functional groups of pre-synthesized MOFs, enzyme molecules were attached on the surface of MOFs (Jung et al. 2011; Shih et al. 2012). Alternatively, another strategy is in situ one-pot synthesis of enzyme–MOF composites directly from enzyme, metal ions, and organic ligands in solution, which has been investigated by several groups (Lyu et al. 2014; Shieh et al. 2015; Liang et al. 2015a; Lian et al. 2016; Liao et al. 2017). All the above methods majorly rely on solution-based procedures. However, the utilization of enzyme–MOF composites in biosensors, bioelectronic devices, and other biomedical applications usually requires the patterned localization of biological components on special surface of devices. And it is challenging to precisely assemble or deposit the as-prepared enzyme–MOF composites with sizes ranging from nanometers to micrometers on substrate surface. Therefore, a scalable and controllable technique of readily positioning enzyme–MOF composites on substrate surface with designable patterns would immediately advance the fabrication and practical application of enzyme–MOF composites in biosensors, wearable bioelectronic devices, artificial biomimetic membranes, and tissue engineering.

Stimulated by the in situ solution synthesis of enzyme–MOF composites developed previously (Lyu et al. 2014; Wu et al. 2015a, b; Liang et al. 2015a), in this study, for the first time, we use a commercially available color ink-jet printer to directly print enzyme–MOF composites on the surface of different substrates including filter paper, polyvinylchloride (PVC) film, poly(ethylene terephthalate) (PET) film, and hydrophilic printing film. Ink solutions containing zinc ions (Zn2+), 2-methylimidazole (2-MeIM), and enzyme molecules were loaded in different cartridges, respectively (C, M, Y cartridges as shown in procedure ① of Fig. 1). Droplets of ink solutions were sprayed on the surface of printing substrates, allowing the mixing of precursors and in situ growing of enzyme–MOF composites in the merged droplets on the surface before complete evaporation or infiltration of droplets. The mixing ratio of precursors and patterning of printed composites on substrates can be precisely designed by a computer controlling the printer. This synthesis technique using a commercially available color ink-jet printer (with price around 200 US dollars) allowed a facile and low-cost method to fabricate patterned enzyme–MOF composites on different substrates with dozens to hundreds of copies within minutes.

Fig. 1
figure1

a, b Printed patterns formed by ZIF-8 crystals on filter paper, and patterns (approximately 5 cm × 5 cm) are badges of Tsinghua University. SEM images of pure ZIF-8 particles printed on (c, d) filter paper (8 times printing), e PVC film (8 times printing), f PET film (8 times printing), g hydrophilic printing film (1 time printing). h SEM image of ZIF-8 particles grown on PET film printed with protein and a pattern (i) (approximately 5 cm × 3 cm) of a monkey representing lunar year of 2016 formed by ZIF-8 particles grown on PET film printed with protein. Organic ligand 2-MeIM is abbreviated as OL in the figure

Full size image

Experimental section

Materials

Bovine serum albumin (BSA), 2-methylimidazole, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt (ABTS), dimethyl sulphoxide (DMSO), ethanol, ethylene glycol, tertiary butanol, Triton X-100, fluorescein isothiocyanate (FITC), and rhodamine B were purchased from Sigma-Aldrich. Zinc nitrate hexahydrate, zinc acetate, hydrogen peroxide (H2O2) were purchased from Alfa Aesar. Cytochrome c (Cyt c) from equine heart and horseradish peroxidase were purchased from Hoffmann-La Roche. Other chemicals are of analytical grade.

Printing procedure was carried out on a piezoelectric ink-jet printer Epson ME-10. The four ink cartridges were filled with self-formulated enzyme or precursors solutions; the graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space. Whatman No. 1 filter paper was purchased from Sigma-Aldrich. Polyvinylchloride (PVC) film and poly(ethylene terephthalate) (PET) film were produced by Empero Co., LTD. and hydrophilic printing film was produced by FULEJET Co., LTD.

Preparation of ink solutions

Four different formulations of inks (Additional file 1: Table S1) were prepared to obtain an appropriate surface tension and viscosity in the range of 30–50 mN/m and 2–5 mPa s, which is required for a smooth and continuous ink-jet printing. The viscosity measurement was carried out on a Physica MCR 301 rheometer under a shearing rate at 100/s at 25 °C. Inks 1–3 were used to dissolve and print precursors of ZIF-8 at required concentrations, and Ink 4 was used to dissolve and print protein and other molecules at required concentrations.

The inks were prepared before printing and filtered through a 0.45-μm membrane prior to filling in the color cartridges. After each printing experiment, an automatic washing produce of the printer was conducted to avoid the block of nozzle by aggregates of metal ions, protein molecules, or other molecules.

For the investigation of formulation of inks, Inks 1–3 were used to dissolve zinc nitrate hexahydrate (Zn2+) and 2-methylimidazole (2-MeIM) at a concentration of 125 mmol/L and 1 mol/L, respectively. The ink solution containing Zn2+ was loaded in the C cartridge and the ink solution containing 2-MeIM was loaded in the M cartridge. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing C = 100% and M = 100%. The patterns to be printed were 3 cm × 3 cm squares. The printing substrate was filter paper.

Investigation of different printing substrates and printing times

For the investigation of printing of pure ZIF-8 on different substrates including filter paper, PVC film, PET film, and hydrophilic film, Ink 1 was used to dissolve zinc nitrate hexahydrate (Zn2+) and 2-methylimidazole (2-MeIM) at a concentration of 125 mmol/L and 1 mol/L, respectively. The ink solution containing Zn2+ was loaded in the C cartridge and the ink solution containing 2-MeIM was loaded in the M cartridge. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing C = 100% and M = 100%. The patterns to be printed were 3 cm × 3 cm2. Various times of printing (interval time between each printing was 5 min) were achieved by repeating the printing on the same pattern.

Investigation of precursors concentrations and printing ratios

For the investigation of concentrations of Zn2+ and 2-MeIM, Ink 1 was used to dissolve zinc nitrate hexahydrate (Zn2+) and 2-methylimidazole (2-MeIM) at a concentration of 125 mmol/L (or 62.5 mmol/L) and 1 mol/L (or 0.5 mol/L), respectively. The ink solution containing Zn2+ was loaded in the C cartridge and the ink solution containing 2-MeIM was loaded in the M cartridge. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing C = 100% and M = 100% (Zn2+/2-MeIM molar ratio 1:8), or fixing C = 100% and M = 50% (Zn2+/2-MeIM molar ratio 1:4). The patterns to be printed were 3 cm × 3 cm squares. The printing substrate was filter paper.

Printed protein-induced formation of ZIF-8

Ink 4 was used to dissolve BSA at a concentration of 50 mg/mL. Then the protein ink was loaded in Y cartridge and printed on PET film with a predesigned pattern. After drying the film with printed protein pattern at room temperature for 4 h, the film was immersed in a solution containing 20 mmol/L of zinc acetate and 80 mmol/L 2-methylimidazole solution allowing reaction for 8 h. After washing with DI water to remove unreacted metal ions and organic ligands, the film was dried at room temperature for 2 h. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing Y = 100%.

Printing Cyt c–ZIF-8 composites

Ink 1 was used to dissolve zinc nitrate hexahydrate (Zn2+) and 2-methylimidazole (2-MeIM) at a concentration of 125 mmol/L and 1 mol/L, respectively. Ink 4 was used to dissolve Cyt c (or Cyt c-FITC) at a concentration of 4 mg/mL. Then inks containing Zn2+, 2-MeIM, and protein were loaded in C, M, Y cartridges and printed on hydrophilic film with a predesigned pattern. With this order of cartridges, protein molecules can induce the formation of ZIF-8. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing C = 100%, M = 100%, and Y = 100%.

Ink 4 was used to dissolve rhodamine B at a concentration of 4 mg/mL and was loaded in K cartridge. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing C = 100%, M = 100%, and Y = 100% and adjusting the percentage of K from 0 to 100% (0, 20, 40, 60, 80, 100%).

Printing Cyt c–ZIF-8 composites on filter papers to construct testing strips

Ink 1 was used to dissolve zinc nitrate hexahydrate (Zn2+) and 2-methylimidazole (2-MeIM) at a concentration of 125 mmol/L and 1 mol/L, respectively. Ink 4 was used to dissolve Cyt c at a concentration of 4 mg/mL. Then inks containing Zn2+, 2-MeIM, and protein were loaded in C, M, Y cartridges and printed on filter papers as round patterns with diameters of 6 mm. The graphics files to be printed were prepared by Adobes Photoshops CC software in CMYK color space by fixing C = 100%, M = 100%, and Y = 100%.

Each round piece was utilized as a testing strip. 10 μL of ABTS (2.8 mg/mL in water) and 10 μL of H2O2 solution with different concentrations were dropped on the round pieces. Once the round strips turned green after 2 min, the color intensity (gray value) was analyzed using the Image J2x software.

Results and discussion

The synthesis of pure MOF crystals was first investigated by the ink-jet printing method, loading inks containing Zn2+ and 2-MeIM in C and M cartridges and printing the ink on substrate according to predesigned patterns. Patterns designed by computer can easily be generated by the in situ formed ZIF-8 particles on surface by the ink-jet printing. Figure 1a, b shows the photos of printed badges of Tsinghua University produced by in situ formed ZIF-8 particles. Figure 1c–g shows scanning electron microscope (SEM) images of printed MOF crystals on different substrates including filter paper, PVC film, PET film, and hydrophilic printing film. ZIF-8 crystals with regular shapes similar as that produced in solution (Lyu et al. 2014; He et al. 2016) were in situ formed on substrate surface, as exemplified in Fig. 1d. Surface powder X-ray diffraction (XRD) pattern (Additional file 1: Figure S1) suggests that the printed ZIF-8 has the same crystalline structure as that synthesized in solution (Lyu et al. 2014; He et al. 2016). Systematic studies revealed that inks with an appropriate surface tension and viscosity in the range of 30–50 mN/m and 2–5 mPa s are ideal for continuous printing (Additional file 1: Table S1). A combination of dimethyl sulfoxide (DMSO), ethanol, and ethylene glycol (at a volume ratio of 4:9:6) was the best solvent among others to print precursors of ZIF-8, and an optimized Zn2+/2-MeIM printing molar ratio of 1/8 produced more ZIF-8 particles (Additional file 1: Figures S2, S3). In addition, each substrate can be repeatedly printed for several times to increase the productivity of ZIF-8 particles on substrate surface (Additional file 1: Figure S4). As shown in Fig. 1c–f, the average size of the crystals formed on filter paper, PVC, and PET was 100, 70, and 30 nm, respectively. In addition, denser particles were formed on filter paper than on PVC and PET films. According to the mechanism of a color ink-jet printer, droplets of inks in different cartridges are separately sprayed on surface and droplets are merged together once deposited on surface. Therefore, it is possible that more hydrophilic surface (for example, filter paper made of cellulose fibers) can facilitate the merging of different droplets containing Zn2+ or 2-MeIM to generate more ZIF-8 particles with larger particle size. To further demonstrate this hypothesis, we printed ZIF-8 particles on hydrophilic printing film by only one time and a high density of ZIF-8 particles was readily achieved (Fig. 1g). Here, with the above results, we can conclude that MOF crystals can be readily formed on the surface of substrate by ink-jet printing and a hydrophilic environment is helpful for the formation of MOF crystals.

Therefore, we wonder if the presence of protein molecules which are hydrophilic biomacromolecules would also advance the formation of MOF crystals on surface. Thus, to investigate the effect of protein, another ink-jet printing process was utilized as shown in procedure ② of Fig. 1. Protein molecules (bovine serum albumin, BSA) were first printed on PET film and created areas with more hydrophilic surface. Then, when immersing the protein-printed PET film in solution containing Zn2+ and 2-MeIM, dense ZIF-8 particles were in situ formed (Fig. 1h) on the protein layer. To prove that ZIF-8 particles were preferably formed on the protein layer, a pattern of a monkey was constructed by printing protein molecules on a hydrophilic film, followed by immersing the film in aqueous solution containing Zn2+ and 2-MeIM. After this treatment, a clear and vivid pattern of monkey (Fig. 1i) was created due to the in situ formation of ZIF-8. SEM image of the cross section revealed that the thickness of the MOF layer was about 20 µm (Fig. 2a) and the MOF layer was observed consisting of many ZIF-8 nanoparticles with size around 100 nm at a higher resolution under SEM (Fig. 2b). After ink-jet printing protein as patterned spots with size around 200 µm on hydrophilic film, the film was immersed in aqueous solution containing Zn2+, 2-MeIM, and rhodamine B. It is clearly observed that ZIF-8 was precisely formed on the region where protein was printed (Fig. 2c) and the fluorescent molecule rhodamine B was incorporated in ZIF-8. This precisely formation of MOFs on protein patterns proves that hydrophilic protein molecules can induce the formation of MOFs (Liang et al. 2015b). These results prove that the presence of protein molecules highly facilitates the synthesis of MOF.

Fig. 2
figure2

SEM images of a ZIF-8 layer and b ZIF-8 nanoparticles formed on PET film printed with protein. c, d SEM and fluorescent image of rhodamine B-incorporated ZIF-8 formed on PET film printed with protein spots. e SEM image of Cyt c–ZIF-8 composites on hydrophilic film by one-time printing. f, g Bright-field image and fluorescent image of FITC-labeled Cyt c–ZIF-8 composites on hydrophilic film. h Fluorescent images of rhodamine B-incorporated Cyt c–ZIF-8 composites on hydrophilic film (up to down: 0, 20, 40, 60, 80, 100% K value, while C, M, Y values are 100% as constant). Scale bar is 500 Âµm long in (h)

Full size image

Last, we carried out the process of printing enzyme and MOF precursors at the same time to form the enzyme–MOF composites on surface directly. Ink solutions containing 4 mg/mL Cytochrome c (Cyt c), 125 mM Zn2+, and 1 M 2-MeIM were loaded in different cartridges (C, M, Y cartridges as shown in procedure ① of Fig. 1) and were directly printed on the surface of hydrophilic film. SEM image in Fig. 2e shows that just one-time printing forms a high density of nanoparticles with size of ~ 100 nm, which are similar as the ZIF-8 crystals prepared by the protein-induced synthesis (Fig. 1h, 2b). Surface powder X-ray diffraction (XRD) pattern (Additional file 1: Figure S1) suggests that the printed Cyt c–ZIF-8 composites have crystalline composition similar as pure ZIF-8; however, due to the small particle size and the presence of protein molecules, the XRD pattern shows widened peaks and the existence of amorphous composition. To prove the successful incorporation of protein in the composites, Cyt c was first labeled with fluorescein isothiocyanate (FITC) and subjected to the same printing process. The hydrophilic film printed with protein–ZIF-8 composites was then washed with DI water to remove the unreacted Zn2+, 2-MeIM and free Cyt c. By measuring the fluorescence intensity of Cyt c-FITC in the supernatant, the incorporation ratio of protein was calculated as ~ 75%. This result indicates that most of the protein is embedded in the ZIF-8 particles instead of being absorbed on the surface of MOF layer. In another word, the protein molecules induce the formation of ZIF-8 particles around. To further calculate the weight percentage of protein in the Cyt c–ZIF-8 composites, small pieces of the printed hydrophilic film were immersed in acid solution to dissolve the composites, followed by the determination of Fe and Zn amounts through inductively coupled plasma mass spectrometry (ICP–MS) (Additional file 1: Table S2). As there is one equiv of Fe per protein, the weight percentage of Cyt c in composites was calculated to be around 15%. The fluorescent image of the FITC-labeled Cyt c–ZIF-8 composites on hydrophilic film after washing (Fig. 2f, g) again proves the incorporation of protein with a dense and equal distribution.

From the above results, we conclude that the formation of enzyme–MOF composites on substrate surface with predesigned patterns can be easily achieved by a color ink-jet printing technique and the hydrophilic surface facilitates the formation of ZIF-8 particles possibly due to the enhanced merging process of microdroplets on hydrophilic surface. Moreover, the presence of protein in printing process further increases the hydrophilic nature of the microdroplets and the surface, thus further facilitates the formation of ZIF-8 particles with a high density on the hydrophilic surface by just one-time printing (Fig. 2e). Protein molecules, Cyt c, can be easily incorporated in ZIF-8 particles by this printing technique as they induce the formation of ZIF-8 in merged microdroplets. In addition to protein incorporation, the color ink-jet printing technique is expandable to using more cartridges to incorporate other functional components in composites with a predesigned ratio at the same time (Zhang et al. 2014a, b). For example, rhodamine B was loaded in K cartridge and encapsulated in the Cyt c–ZIF-8 composites. As shown in Fig. 2h, with the preset printing ratio of K cartridge increased from 0 to 100%, the red fluorescence was observed to be increased accordingly, suggesting a controllable encapsulation by this printing technique.

As an application of this printing technique in scalable and designable fabrication of biosensors with low cost, Cyt c–ZIF-8 composite was printed on filter paper as round patterns with diameters of 6 mm. Each round piece was utilized as a testing strip for H2O2 in solution. The incorporated Cyt c can catalyze the oxidation of ABTS by H2O2, with forming a green colored product (Fig. 3a) which can be determined by naked eyes or a color intensity analysis software. For a typical testing, 10 μL of 2,2′-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) (2.8 mg/mL in water) and 10 μL of H2O2 solution with different concentrations were dropped on the round pieces. Once the testing strips turned green after 2 min, the intensity of the color directly reflected the concentration of H2O2 in solution (Fig. 3b). In contrast, the same amount of native Cyt c printed on the same round piece without ZIF-8 cannot catalyze the reaction and produce any color change in the same detection time due to the low peroxidase activity of native Cyt c. The Cyt c incorporated in ZIF-8 shows enhanced activity compared to native Cyt c, reasons of which are majorly the activation effect of Zn2+, increased apparent affinity of Cyt c–ZIF-8 composite towards H2O2, and the more exposed active site of the incorporated Cyt c as investigated by several previous studies (Lyu et al. 2014; Zhang et al. 2017). The detection range of the testing strips is from 20 to 120 mM, with a limit of 20 mM by visible detection, making it promising for fast and convenient determination of H2O2 in solution. Detection of H2O2 is important in food, pharmaceutical, textile industries, and environmental protection (Zhang et al. 2014b; Jv et al. 2010). As an additive for microbial inhibiting or bleaching, H2O2 has been used in food industry (Nascimento et al. 2017). Due to the toxicity of residual H2O2, the presence of H2O2 is not allowed in food under European Union and Japanese regulations (Hsu et al. 2008). Food and Drug Administration (FDA) limits H2O2 to a very low concentration (Code of Federal Regulations 2017) in finished food packages. The low-cost testing strips produced by the scalable printing technique could be useful in analysis and regulation in food industry.

Fig. 3
figure3

a Schematic illustration of detection of H2O2 by the Cyt c–ZIF-8 composite testing strip. b Color intensity (gray value) of testing strips developed for different concentrations of H2O2

Full size image

Conclusions

In summary, we explored a direct synthesis of enzyme–MOF composites by ink-jet printing for the first time. By loading MOF precursors and protein in different cartridges of color printer and printing the inks according to predesigned patterns, protein–MOF composites can be easily in situ generated on the surface of various substrates such as paper and polymeric films due to the merging of microdroplets containing precursors and protein on surface. The results show that the printed MOF and protein–MOF composites can highly preserve the morphology and crystalline structure similar as pure MOF synthesized in solution. Hydrophilic surface and the presence of hydrophilic protein facilitates the formation of a high density of MOF on surface. As an example, the printed Cyt c–ZIF-8 composites on filter paper exhibits higher activity than native protein and can be used as low-cost testing strips for fast and facile H2O2 detection in food industry. This technique provides new possibilities of scalable, controllable, and designable fabrication of functional protein–MOF hybrid surface with many important applications in fields such as biosensing, wearable bioelectronics, artificial biomimetic membranes, and tissue engineering.

Abbreviations

MOF:

metal–organic framework

Cyt c:

Cytochrome c

ZIF-8:

zeolitic imidazolate frameworks-8

SEM:

scanning electron microscope

TEM:

transmission electron microscope

XRD:

X-ray diffraction

2-MeIM:

2-methylimidazole

BSA:

bovine serum albumin

ABTS:

2-methylimidazole, 2,2′-azino-bis(3-ethylbenzothiazoline-6-sulfonic acid) diammonium salt3

PVC:

polyvinylchloride

PET:

poly(ethylene terephthalate)

References

  1. Chen Y, Lykourinou V, Vetromile C, Hoang T, Ming L, Larsen RW, Ma S (2012) How can proteins enter the interior of a MOF? Investigation of Cytochrome c translocation into a MOF consisting of mesoporous cages with microporous windows. J Am Chem Soc 134:13188–13191. doi:10.1021/ja305144x

    CAS  Article  Google Scholar 

  2. Code of Federal Regulations 21CFR178.1005 (2017). https://www.accessdata.fda.gov/scripts/cdrh/cfdocs/cfcfr/CFRSearch.cfm. Accessed 1 Apr 2017

  3. Deng H, Grunder S, Cordova KE, Valente C, Furukawa H, Hmadeh M, Gándara F, Whalley AC, Liu Z, Asahina S, Kazumori H, O’Keeffe M, Terasaki O, Stoddart JF, Yaghi OM (2012) Large-pore apertures in a series of metal-organic frameworks. Science 336:1018–1023. doi:10.1126/science.1220131

    CAS  Article  Google Scholar 

  4. Feng D, Liu T, Su J, Bosch M, Wei Z, Wan W, Yuan D, Chen Y, Wang X, Wang K (2015) Stable metal-organic frameworks containing single-molecule traps for enzyme encapsulation. Nat Commun 6:5979. doi:10.1038/ncomms6979

    Article  Google Scholar 

  5. Furukawa H, Cordova KE, O’Keeffe M, Yaghi OM (2013) The chemistry and applications of metal-organic frameworks. Science 341:1230444. doi:10.1126/science.1230444

    Article  Google Scholar 

  6. Hayashi H, Cote AP, Furukawa H, O’Keeffe M, Yaghi OM (2007) Zeolite a imidazolate frameworks. Nat Mater 6:501–506. doi:10.1038/nmat1927

    CAS  Article  Google Scholar 

  7. He H, Han H, Shi H, Tian Y, Sun F, Song Y, Li Q, Zhu G (2016) Construction of thermophilic lipase-embedded metal-organic frameworks via biomimetic mineralization: a biocatalyst for ester hydrolysis and kinetic resolution. ACS Appl Mater Interfaces 8:24517–24524. doi:10.1021/acsami.6b05538

    CAS  Article  Google Scholar 

  8. Hou M, Ge J (2017) Armoring enzymes by metal-organic frameworks by the coprecipitation method. Methods Enzymol 590:59–75. doi:10.1016/bs.mie.2016.12.002

    Article  Google Scholar 

  9. Hsu C, Chang K, Kuo J (2008) Determination of hydrogen peroxide residues in aseptically packaged beverages using an amperometric sensor based on a palladium electrode. Food Control 19:223–230. doi:10.1016/j.foodcont.2007.01.004

    CAS  Article  Google Scholar 

  10. Jung S, Kim Y, Kim S, Kwon TH, Huh S, Park S (2011) Bio-functionalization of metal-organic frameworks by covalent protein conjugation. Chem Commun (Camb) 47:2904–2906. doi:10.1039/c0cc03288c

    CAS  Article  Google Scholar 

  11. Jv Y, Li B, Cao R (2010) Positively-charged gold nanoparticles as peroxidiase mimic and their application in hydrogen peroxide and glucose detection. Chem Commun 46:8017–8019. doi:10.1039/c0cc02698k

    Article  Google Scholar 

  12. Li P, Moon SY, Guelta MA, Harvey SP, Hupp JT, Farha OK (2016a) Encapsulation of a nerve agent detoxifying enzyme by a mesoporous zirconium metal-organic framework engenders thermal and long-term stability. J Am Chem Soc 138(26):8052–8055. doi:10.1021/jacs.6b03673

    CAS  Article  Google Scholar 

  13. Li P, Moon SY, Guelta MA, Lin L, Gómez-Gualdrón DA, Snurr RQ, Harvey SP, Hupp JT, Farha OK (2016b) Nanosizing a metal-organic framework enzyme carrier for accelerating nerve agent hydrolysis. ACS Nano 10(10):9174–9182. doi:10.1021/acsnano.6b04996

    CAS  Article  Google Scholar 

  14. Li S, Dharmarwardana M, Welch RP, Ren Y, Thompson CM, Smaldone RA, Gassensmith JJ (2016c) Template-directed synthesis of porous and protective core-shell bionanoparticles. Angew Chem 128(36):10849–10854. doi:10.1002/ange.201604879

    Article  Google Scholar 

  15. Lian X, Chen Y, Liu T, Zhou H (2016) Coupling two enzymes into a tandem nanoreactor utilizing a hierarchically structured MOF. Chem Sci 7:6969–6973. doi:10.1039/c6sc01438k

    CAS  Article  Google Scholar 

  16. Lian X, Fang Y, Joseph E, Wang Q, Li J, Banerjee S, Lollar C, Wang X, Zhou H (2017) Enzyme-MOF (metal-organic framework) composites. Chem Soc Rev 46:3386–3401. doi:10.1039/c7cs00058h

    CAS  Article  Google Scholar 

  17. Liang K, Carbonell C, Styles MJ, Ricco R, Cui J, Richardson JJ, Maspoch D, Caruso F, Falcaro P (2015a) Biomimetic replication of microscopic metal-organic framework patterns using printed protein patterns. Adv Mater 27:7293–7298. doi:10.1002/adma.201503167

    CAS  Article  Google Scholar 

  18. Liang K, Ricco R, Doherty CM, Styles MJ, Bell S, Kirby N, Mudie S, Haylock D, Hill AJ, Doonan CJ (2015b) Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules. Nat Commun. doi:10.1038/ncomms8240

    Google Scholar 

  19. Liao FS, Lo WS, Hsu YS, Wu CC, Wang SC, Shieh FK, Morabito JV, Chou LY, Wu KCW, Tsung CK (2017) Shielding against unfolding by embedding enzymes in metal-organic frameworks via a de novo approach. J Am Chem Soc 139(19):6530–6533. doi:10.1021/jacs.7b01794

    CAS  Article  Google Scholar 

  20. Lykourinou V, Chen Y, Wang XS, Meng L, Hoang T, Ming LJ, Musselman RL, Ma S (2011) Immobilization of MP-11 into a mesoporous metal-organic framework, MP-11@mesoMOF: a new platform for enzymatic catalysis. J Am Chem Soc 133:10382–10385. doi:10.1021/ja2038003

    CAS  Article  Google Scholar 

  21. Lyu F, Zahng Y, Zare RN, Ge J, Liu Z (2014) One-pot synthesis of protein-embedded metal-organic frameworks with enhanced biological activities. Nano Lett 14:5761–5765. doi:10.1021/nl5026419

    CAS  Article  Google Scholar 

  22. Nascimento CF, Santos PM, Pereira-Filho ER, Rocha FR (2017) Recent advances on determination of milk adulterants. Food Chem 221:1232–1244. doi:10.1016/j.foodchem.2016.11.034

    CAS  Article  Google Scholar 

  23. Shieh FK, Wang SC, Yen C, Wu C, Dutta S, Chou L, Morabito JV, Hu P, Hsu MH, Wu KCW (2015) Imparting functionality to biocatalysts via embedding enzymes into nanoporous materials by a de novo approach: size-selective sheltering of catalase in metal-organic framework microcrystals. J Am Chem Soc 137(13):4276–4279. doi:10.1021/ja513058h

    CAS  Article  Google Scholar 

  24. Shih YH, Lo SH, Yang N, Singco B, Cheng Y, Wu C, Chang I, Huang H, Lin C (2012) Trypsin-immobilized metal-organic framework as a biocatalyst in proteomics analysis. ChemPlusChem 77:982–986. doi:10.1002/cplu.201200186

    CAS  Article  Google Scholar 

  25. Sumida K, Rogow DL, Mason JA, McDonald TM, Bloch ED, Herm ZR, Bae TH, Long JR (2011) Carbon dioxide capture in metal-organic frameworks. Chem Rev 112:724–781. doi:10.1021/cr2003272

    Article  Google Scholar 

  26. Wu X, Ge J, Yang C, Hou M, Liu Z (2015a) Facile synthesis of multiple enzyme-containing metal-organic frameworks in a biomolecule-friendly environment. Chem Commun (Camb) 51:13408–13411. doi:10.1039/c5cc05136c

    CAS  Article  Google Scholar 

  27. Wu X, Hou M, Ge J (2015b) Metal–organic frameworks and inorganic nanoflowers: a type of emerging inorganic crystal nanocarrier for enzyme immobilization. Catal Sci Technol 5:5077–5085. doi:10.1039/c5cy01181g

    CAS  Article  Google Scholar 

  28. Wu X, Yang C, Ge J (2017) Green synthesis of enzyme/metal-organic framework composites with high stability in protein denaturing solvents. Bioresour Bioprocess 4:24. doi:10.1186/s40643-017-0154-8

    Article  Google Scholar 

  29. Zhang Y, Bai X, Wang X, Shiu KK, Zhu Y, Jiang H (2014a) Highly sensitive graphene-Pt nanocomposites amperometric biosensor and its application in living cell H2O2 detection. Anal Chem 86:9459–9465. doi:10.1021/ac5009699

    CAS  Article  Google Scholar 

  30. Zhang Y, Lyu F, Ge J, Liu Z (2014b) Ink-jet printing an optimal multi-enzyme system. Chem Commun 50:12919–12922. doi:10.1039/c4cc06158f

    CAS  Article  Google Scholar 

  31. Zhang C, Wang X, Hou M, Li X, Wu X, Ge J (2017) Immobilization on metal-organic framework engenders high sensitivity for enzymatic electrochemical detection. ACS Appl Mater Interfaces 9(16):13831–13836. doi:10.1021/acsami.7b02803

    CAS  Article  Google Scholar 

  32. Zhou HC, Long JR, Yaghi OM (2012) Introduction to metal-organic frameworks. Chem Rev 112:673–674. doi:10.1021/cr300014x

    CAS  Article  Google Scholar 

Download references

Authors’ contributions

MH and JG designed the experiments. MH and HZ performed the experiments. HZ, YF, XW, and JG drafted the manuscript. MH, HZ, and JG contributed to the discussion. All authors read and approved the final manuscript.

Acknowledgements

Not applicable.

Competing interests

The authors declare that they have no competing interests.

Availability of data and materials

All data generated or analyzed during this study are included in this articles.

Consent for publication

Not applicable.

Ethics approval and consent to participate

Not applicable.

Funding

This work was supported by the National Key Research and Development Plan of China (2016YFA0204300), and the National Natural Science Foundation of China (21622603, 51573085).

Publisher’s Note

Springer Nature remains neutral with regard to jurisdictional claims in published maps and institutional affiliations.

Author information

Affiliations

  1. Key Lab for Industrial Biocatalysis, Ministry of Education, Department of Chemical Engineering, Tsinghua University, Beijing, 100084, China

    Miao Hou, Yi Feng & Jun Ge

  2. Beijing National Day School, Beijing, 100039, China

    Haotian Zhao

Authors
  1. Miao Hou
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Haotian Zhao
    View author publications

    You can also search for this author in PubMed Google Scholar

  3. Yi Feng
    View author publications

    You can also search for this author in PubMed Google Scholar

  4. Jun Ge
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Jun Ge.

Additional file

Additional file 1.

Supporting information for the detailed experimentals and characterization.

Rights and permissions

Open Access This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made.

Reprints and Permissions

About this article

Verify currency and authenticity via CrossMark

Cite this article

Hou, M., Zhao, H., Feng, Y. et al. Synthesis of patterned enzyme–metal–organic framework composites by ink-jet printing. Bioresour. Bioprocess. 4, 40 (2017). https://doi.org/10.1186/s40643-017-0171-7

Download citation

Keywords

  • Immobilized enzyme
  • Metal–organic frameworks
  • Ink-jet printing