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Bioresources and Bioprocessing
Fig. 4 | Bioresources and Bioprocessing

Fig. 4

From: Characterization and overexpression of a novel keratinase from Bacillus polyfermenticus B4 in recombinant Bacillus subtilis

Fig. 4

a Batch fermentation of recombinant KerP-producing strain PT5(DMT01-mKerP) in a 5-L fermenter. Cell density was determined during fermentation (closed circles) at OD600, and the production of KerP was assayed through measurement of keratinolytic activity (open circles). b SDS-PAGE analysis of KerP secreted by recombinant B. subtilis PT5(DMT01-mKerP). Fifteen microliters of culture broth from different fermentation periods was drawn out for PAGE. Lanes M, protein marker; lane 1, the culture broth of PT5 after 24-h fermentation; the culture broth of strain PT5(DMT01-mKerP) after fermentation for lane 2, 3 h; lane 3, 6 h; lane 4, 9 h; lane 5, 12 h; lane 6, 15 h; lane 7, 18 h; lane 8, 21 h; and lane 9, 24 h. The arrow indicates the position of KerP

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