Table 1 Strains and plasmids used in this study
Relevant characteristics | Source | |
|---|---|---|
Strain | ||
E. coli | ||
DH5α | deoR endA1 gyrA96 hsdR17 supE44 thi1 recA1 lacZM15 | Lab. collection |
B. subtilis | ||
PT5 | DB428, △wpr::T7gene 1 | |
PT5(DMT-KerP) | PT5, △mpr:: PT7/kerP-erm | This study |
PT5(DMT1-mKerP) | PT5, △mpr:: PT7/aprE SP-mkerp-erm | This study |
PT5(DMT7-mKerP) | PT5, △mpr:: PT7/xynD SP-mkerp-erm | This study |
PT5(DMT8-mKerP) | PT5, △mpr:: PT7/bglS SP-mkerp-erm | This study |
PT5(DMT12-mKerP) | PT5, △mpr:: PT7/mpr SP-mkerp-erm | This study |
PT5(DMT16-mKerP) | PT5, △mpr:: PT7/xynA SP-mkerp-erm | This study |
PT5(DMT21-mKerP) | PT5, △mpr:: PT7/abnA SP-mkerp-erm | This study |
Plasmid | ||
pDMT | Integration vector contains by PT7 and mpr′ | |
pDMT-KerP | pDMT with full-length kerP gene | This study |
pDMT1-mKerP | aprE SP instead of kerP SP in pDMT-KerP | This study |
pDMT7-mKerP | xynD SP instead of kerP SP in pDMT-KerP | This study |
pDMT8-mKerP | bglS SP instead of kerP SP in pDMT-KerP | This study |
pDMT12-mKerP | mpr SP instead of kerP SP in pDMT-KerP | This study |
pDMT16-mKerP | xynA SP instead of kerP SP in pDMT-KerP | This study |
pDMT21-mKerP | abnA SP instead of kerP SP in pDMT-KerP | This study |