Fig. 6
The performance of cell surface display for BsCel5. A Congo red tests for cells harboring plasmids pET28a (a), pET28a-BsCel5 (b), pET28a-Ag43-138-BsCel5 (c), and pET28a-Ag43-700-BsCel5 (d). Left and right images represent before and after congo red staining, respectively. B Whole-cell activity assay for cells harboring pET28a-BsCel5, pET28a-Ag43-138-BsCel5, and pET28a-Ag43-700-BsCel5. The activity was measured by the amount of unit possessed per milliliter culture (OD600 nm of 2). One unit of activity was defined as the amount of glucose generated per minute. The activity of pET28a-BsCel5 was 0.27 U/mL and defined as 1. C Thermal stability of BsCel5 in the different recombinants. The activities of BsCel5 at 55, 60, 70, and 80 °C are indicated with stacked bars in green, pink, gray, and yellow (from top to down), respectively