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Table 3 Plackett–Burman for alkali-pretreated substrate

From: Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium

Run orderExperimental parametersEnzyme production (IU/ML)a
Temperature (°C)pHMoistening agentIncubation timeInoculum size (mL g−1)Moisture content (%)PretreatmentEGBGLXylPECAMY
1307MSM480.2580Alk2.562.764.6218.093.89
2357YEP480.2550Alk5.324.488.6420.769.37
3355YEP720.2580Nil9.2214.8411.1226.4717.86
4305MSM720.580Alk4.045.677.8738.314.54
5357MSM480.550Alk4.045.677.5713.814.63
6357YEP720.2550Nil9.3718.511.3615.6712.97
7307YEP720.580Nil10.9515.5912.0118.6417.02
8307YEP480.580Alk3.794.546.4421.577.30
9355YEP480.2580Alk3.254.735.9726.385.56
10357MSM720.2580Alk6.808.3910.4717.744.83
11355YEP720.550Alk6.759.5215.2623.268.09
12357MSM720.580Nil9.6612.638.8825.5613.41
13305YEP720.550Alk5.877.3114.5530.836.26
14355MSM480.580Nil7.648.3414.2614.5917.66
15305MSM720.2550Alk6.468.3815.4226.647.99
16355MSM480.550Nil11.8412.4814.658.8415.59
17307MSM720.2550Nil5.1812.457.39214.409.72
18307YEP480.550Nil14.7512.9217.2214.1016.58
19305YEP480.2580Nil12.6213.6718.0614.1016.28
20305MSM480.2550Nil6.018.5315.1010.4013.42
  1. SSF of acid-pretreated Citrus limetta was carried out by yeast consortium, factors affecting multienzyme preparation were adjusted at two levels (temperature 30, 35 °C; pH 5, 7; moistening agent mineral salt medium or MSM, yeast extract and peptone; incubation time 48, 72 h; inoculum size 0.25, 0.5 mL g−1; moisture content 50, 80%; pretreatment acid, no treatment, or nil), and production of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC), and amylase (AMY) was taken as response
  2. aThe values represent average of triplicate with insignificant standard deviation