Bioresources and Bioprocessing

Table 3 Plackett–Burman for alkali-pretreated substrate

From: Citrus limetta peels: a promising substrate for the production of multienzyme preparation from a yeast consortium

Run order	Experimental parameters							Enzyme production (IU/ML)^a
Run order	Temperature (°C)	pH	Moistening agent	Incubation time	Inoculum size (mL g⁻¹)	Moisture content (%)	Pretreatment	EG	BGL	Xyl	PEC	AMY
1	30	7	MSM	48	0.25	80	Alk	2.56	2.76	4.62	18.09	3.89
2	35	7	YEP	48	0.25	50	Alk	5.32	4.48	8.64	20.76	9.37
3	35	5	YEP	72	0.25	80	Nil	9.22	14.84	11.12	26.47	17.86
4	30	5	MSM	72	0.5	80	Alk	4.04	5.67	7.87	38.31	4.54
5	35	7	MSM	48	0.5	50	Alk	4.04	5.67	7.57	13.81	4.63
6	35	7	YEP	72	0.25	50	Nil	9.37	18.5	11.36	15.67	12.97
7	30	7	YEP	72	0.5	80	Nil	10.95	15.59	12.01	18.64	17.02
8	30	7	YEP	48	0.5	80	Alk	3.79	4.54	6.44	21.57	7.30
9	35	5	YEP	48	0.25	80	Alk	3.25	4.73	5.97	26.38	5.56
10	35	7	MSM	72	0.25	80	Alk	6.80	8.39	10.47	17.74	4.83
11	35	5	YEP	72	0.5	50	Alk	6.75	9.52	15.26	23.26	8.09
12	35	7	MSM	72	0.5	80	Nil	9.66	12.63	8.88	25.56	13.41
13	30	5	YEP	72	0.5	50	Alk	5.87	7.31	14.55	30.83	6.26
14	35	5	MSM	48	0.5	80	Nil	7.64	8.34	14.26	14.59	17.66
15	30	5	MSM	72	0.25	50	Alk	6.46	8.38	15.42	26.64	7.99
16	35	5	MSM	48	0.5	50	Nil	11.84	12.48	14.65	8.84	15.59
17	30	7	MSM	72	0.25	50	Nil	5.18	12.45	7.392	14.40	9.72
18	30	7	YEP	48	0.5	50	Nil	14.75	12.92	17.22	14.10	16.58
19	30	5	YEP	48	0.25	80	Nil	12.62	13.67	18.06	14.10	16.28
20	30	5	MSM	48	0.25	50	Nil	6.01	8.53	15.10	10.40	13.42

SSF of acid-pretreated Citrus limetta was carried out by yeast consortium, factors affecting multienzyme preparation were adjusted at two levels (temperature 30, 35 °C; pH 5, 7; moistening agent mineral salt medium or MSM, yeast extract and peptone; incubation time 48, 72 h; inoculum size 0.25, 0.5 mL g⁻¹; moisture content 50, 80%; pretreatment acid, no treatment, or nil), and production of endoglucanase (EG), β-glucosidase (BGL), xylanase (Xyl), pectinase (PEC), and amylase (AMY) was taken as response
^aThe values represent average of triplicate with insignificant standard deviation

Back to article page