Phytochemical screening, metabolite profiling and enhanced antimicrobial activities of microalgal crude extracts in co-application with silver nanoparticle

Bioresources and Bioprocessing

Table 4 Metabolites of the MCEs from different solvent systems and their respective retention times obtained by GC–MS

Compounds	Rt (min)	HEX			MET			ETH
		N. oculata	T. suecica	Chlorella sp.	N. oculata	T. suecica	Chlorella sp.	N. oculata	T. suecica	Chlorella sp.
		(%)	(%)	(%)	(%)	(%)	(%)	(%)	(%)	(%)
Fatty acid
Tetradecanoic acid	29.26				1.06	0.76	2.41
n-Hexadecanoic acid	33.6							5.73	9.75	3.83
9,12,15-Octadecatrienoic acid, (Z,Z,Z)-	37.1							12.08
1-Nonadecene	34.2							3.73		10.74
n-Tetracosanol-1	38.1								1.26	6.76
Ergost-7-en-3-ol	55.7	2.12	14.30	16.58
Ergost-5-en-3-ol, (3.beta.,24r)-	54.7								12.33
Alkane
Eicosane	43.4	3.55	2.67	1.56
Ester
9,12-Octadecadienoic acid, methyl ester	32.07				4.22		1.58
7,10,13-Hexadecatrienoic acid, methyl ester	32.17				5.59	0.48	1.52
Hexadecanoic acid, methyl ester	32.79				5.55	10.92	6.75
9,12,15-Octadecatrienoic acid, methyl ester, (Z,Z,Z)	36.3				16.83	13.23	7.34
Diisooctyl phthalate	43.90	2.23	4.15	2.73	6.28	6.97	12.43
1,2-Benzenedicarboxylic acid, bis (2-methylpropyl) ester	31.4		0.26	12.41
Diethyl Phthalate	25.1							3.40	5.68	2.79
Phenol
2,4-Di-tert butylphenol	23.1				4.06	1.2	0.98
Cont. Phenol, 2,2′-methylenebis[6-(1,1-dimethylethyl-4-methyl-	41.8	6.69		1.69
Terpene
Neophytadiene	30.9	1.77	0.42	11.28	8.96	4.4	6.99	9.24	13.57	19.81
Phytol	36.5	29.49	8.18	14.55				8.73	8.40	6.82
Amide group
13-Docosenamide,(Z)-	47.7	1.62	48.39		17.79	16.01		17.46	9.62
Vitamin
Vitamin E	52.7	1.74
Others
Benzenamine, 4-octyl-N-(4-octylphenyl)-	50.3				8.13	1.94	5.27
Heptasiloxane, hexadecamethyl-	38.2							4.12	11.3
Cyclononasiloxane, octadecamethyl-	45.02							5.77	8.94