Fig. 3
From: Development and fabrication of disease resistance protein in recombinant Escherichia coli
Optimization of DRP protein expression in E. coli. Lane 1: M, lane 2: non-induction by IPTG, lane 3 to 7 are with 0.1 mM IPTG. M, X, W25, W30, C25, C30, and WT mean marker, whole cell which cultured at 25 °C, whole cell which cultured at 30 °C, crude enzyme which cultured at 25 °C, crude enzyme which cultured at 30 °C, and wild type of BL21(DE3). DRP has indicated by red arrow