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Bioresources and Bioprocessing
Fig. 5 | Bioresources and Bioprocessing

Fig. 5

From: Biotechnological applications of S-adenosyl-methionine-dependent methyltransferases for natural products biosynthesis and diversification

Fig. 5

Illustrations of in vivo high-throughput methyltransferases assays. a Growth-coupled methyltransferase assay, where the essential amino acid cysteine (Cys) biosynthesis is linked to SAH biosynthesis. SAH concentration is dependent on methyltransferase activity. If methyltransferase activity is present, Cys will be produced and there is rapid microbial growth. If methyltransferase activity is absent, Cys will not be produced, and no microbial growth is observed. b SAH riboswitch-based biosensor which will bind to 3,5-difluoro-4-hydroxybenzylidene imidazolinone (DFHBI) in the presence of SAH and produce fluorescence signal. c MetJ-MetO system as a biosensor to quantify intracellular SAM concentration in S. cerevisiae. MetJ is fused with an activating domain (AD). In the presence of SAM, metJ will bind to metO and AD will activate the downstream reporter protein expression. d VanR-VanO system as a biosensor to quantify vanillate concentration. In the absence of vanillate, VanR binds to VanO and blocks transcription. In the presence of vanillate, VanR will not be able to bind to VanO, and downstream reporter gene transcription will proceed. Please refer to Table 2 for more information

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