Table 3 Effect of metal ions and chemical reagents on the catalytic activity of Xyl10B
From: Characterization of a novel GH10 alkali-thermostable xylanase from a termite microbiome
Type of additive | Ions or chemical reagent | Relative activity (%) | |||||
|---|---|---|---|---|---|---|---|
1 mM | 10 mM | 1 M | 3 M | 5 M | 0.5% | ||
No addition | 100 | 100 | 100 | 100 | 100 | ||
Salts | Ca2+ (CaCl2) | 90.99 ± 3.45 | 96.98 ± 4.02 | – | – | – | – |
Cu2+ (CuSO4) | 93.00 ± 5.92 | 22.89 ± 3.73 | – | – | – | – | |
Ni2+ (NiCl2) | 94.05 ± 2.22 | 59.88 ± 3.61 | – | – | – | – | |
Mg2+ (MgCl2) | 96.59 ± 2.26 | 105.20 ± 3.05 | – | – | – | – | |
Mn2+ (MnSO4) | 113.7 ± 10.90 | 59.88 ± 3.61 | – | – | – | – | |
Zn2+ (ZnSO4) | 110.84 ± 8.49 | 107.60 ± 1.41 | – | – | – | – | |
Chelating agent | EDTA | 94.64 ± 10.41 | 82.40 ± 5.77 | – | – | – | – |
Detergents | SDS | – | – | – | – | – | 9.59 ± 2.88 |
Tween-40 | – | – | – | – | – | 74.23 ± 8.08 | |
Organic solvents | DMSO | – | – | – | – | – | 80.88 ± 7.57 |
Reducer and denaturing reagent | β-mercaptoethanol | – | – | – | – | – | 52.27 ± 0.58 |
Salt | NaCl | – | – | 93.16± 0.12 | 79.55 ± 3.44 | 64.21 ± 5.46 | – |