Fig. 1

Screening a fluorescent aptamer suitable for in vitro transcription. A Schematic design of the real-time fluorescent assay method for monitoring the RNA synthesis. The DNA template contains a T7 promoter and downstream sequence encoding fluorescent RNA aptamer. B The relative fluorescence units (RFU) generated by five different RNA aptamers were measured under the condition that the excitation wavelength (Ex) was 469 nm and the emission wavelength (Em) was 501 nm. C The fluorescence intensities of five different RNA aptamers were measured under the condition that the Ex was 472 nm and the Em was 507 nm. Error bars represent the standard deviation of the triplicate experiments, P < 0.02