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Bioresources and Bioprocessing
Fig. 3 | Bioresources and Bioprocessing

Fig. 3

From: High-throughput iSpinach fluorescent aptamer-based real-time monitoring of in vitro transcription

Fig. 3

Effects of ribonucleotide triphosphate and MgCl2 concentrations on the STAR system. A Effect of NTP concentrations ranging from 0.5 mM to 6 mM. The relative fluorescent units were measured every 5 min. Error bars are standard errors of the mean (SEM). B Effect of MgCl2 concentrations ranging from 5 to 80 mM. The relative fluorescent units were measured every 6 min. The inset shows the results of denaturing PAGE analysis of the 60-min transcription products under different MgCl2 concentrations. Error bars represent the standard deviation of the triplicate experiments

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