Fig. 6
From: Expression of recombinant human Apolipoprotein A-IMilano in Nicotiana tabacum
A Western blotting of transformants. Lane M is protein molecular weight marker. Lane 1 presents wild N. tabacum. Lane 2 presents recombinant fusion expression of Apo A-IMilano and Flag (Flag–Apo A-IMilano) with a molecular weight of 30 KDa in transient transgenic N. tabacum. Lane 3 shows the expression of the Apo A-I standard protein with a molecular weight of 28 KDa. B Western blot analyser of protein purification. Lane M is protein molecular weight marker. Lane 1 presents total protein recombined expression of Apo A-IMilano and the Flag proposed before purification. Lane 2 presents purified Flag–Apo A-IMilano protein. C Coomassie blue staining of recombinant Flag–Apo A-IMilano proteins in SDS–PAGE. Lane M is standard protein marker. Lane 1 presents Flag–Apo A-IMilano protein purified by 12.5% SDS–PAGE gel. Lane 2 presents the liquid that flows out of the extracted total leaf protein after passing through Protein A/G column, namely, the efflux liquid. Lane 3 presents total leaf protein