Substrate | Km (mM) | kcat (s−1) | kcat/Km (s−1 mM−1) |
---|
ABTS | 0.022 ± 0.007 | 33.05 ± 2.48 | 1495.5 |
NBD | 0.093 ± 0.014 | 17.68 ± 0.72 | 190.0 |
VA | 0.392 ± 0.109 | 11.31 ± 0.61 | 28.9 |
- For each substrate, reactions were performed in triplicate, with monitoring of the increases in absorption for ABTS (ε418 = 36,000 M−1 cm−1), NBD (ε425 = 9700 M−1 cm−1) and veratryl alcohol (ε310 = 9300 M−1 cm−1)