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Construction of an Escherichia coli cell factory to synthesize taxadien-5α-ol, the key precursor of anti-cancer drug paclitaxel

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Paclitaxel (an alkaloid of diterpenoid family) is one of the most widely used anti-cancer drugs. The challenge for biosynthesis of paclitaxel lies on the insufficient precursor, such as taxadien-5α-ol. A recombinant Escherichia coli was constructed with a heterologous mevalonate pathway, a taxadiene synthase from yew, and a cytochrome P450-mediated oxygenation system for the de novo production of taxadien-5α-ol, the first product of the multi-step taxadiene oxygenation metabolism. The final production of total oxygenated taxanes was increased up to 27 mg/L in a 50-mL flask cultivation.

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Bioresources and Bioprocessing (BIOB) is a peer-reviewed open access journal published under the brand SpringerOpen. BIOB aims at providing an international academic platform for exchanging views on and promoting research to support bioresource development, processing and utilization in a sustainable manner. As an application-oriented research journal, BIOB covers not only the application and management of bioresource technology but also the design and development of bioprocesses that will lead to new and sustainable production processes.

BIOB publishes original and review articles on most topics relating to bioresource and bioprocess engineering, including:

  • Biochemical and microbiological engineering
  • Biocatalysis and biotransformation
  • Biosynthesis and metabolic engineering
  • Bioprocess and biosystems engineering
  • Bioenergy and biorefinery
  • Cell culture and biomedical engineering
  • Food, agricultural and marine biotechnology
  • Bioseparation and biopurification engineering
  • Bioremediation and environmental biotechnology

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