Optimization of drying parameters for mango seed kernels using central composite design
© Ekorong et al.; licensee Springer. 2015
Received: 10 September 2014
Accepted: 2 January 2015
Published: 1 March 2015
The combined effect of drying temperature and time was evaluated on residual water content, yield of oil extraction, total phenolic compounds and antioxidant activity of seed kernel from a Cameroonian local variety of mango (Local Ngaoundere). Response surface methodology (RSM) using central composite design (CCD) as tool, was used to develop, validate and optimize statistical models in order to establish the impact of the drying parameters (temperature and time) either alone or in combination.
It was shown that drying temperature individually in its first order (X 1) contributed 30.81, 21.11, 41.28 and 33.24% while drying time individually in its first order (X 2) contributed 39.91, 15.12, 29.92 and 25.87% for residual water content, yield of oil extraction, total phenolic components and antioxidant activity respectively. The increase of drying temperature increased antioxidant activity while the other physicochemical characteristics such as water content, yield of oil extraction and total phenolic components decreased. Concerning drying time, only water content was reduced with an increase of that factor. The synergetic effect of drying temperature and time was effective only for antioxidant activity. A compromise for optimization were then fixed for water content ≤ 10% w/w; oil content ≥ 9% w/w; total polyphenols ≥ 1 mg/g and antioxidant activity ≥ 1000 mg AAE/100 g DM. A simulation for optimization gave, for 60 H and 60°C for drying time and temperature respectively permitted to obtain 4.10% w/w, 9.53% w/w, 1340.28 mg AAE/100 g DM and 1.16 mg/g for water content, oil content, antioxidant activity and total polyphenols respectively.
The physicochemical characteristics studied was globally influenced by the chosen factors (drying time and temperature).
Consumption and industrial exploitation of mango generate significant waste, mainly made of the peels and the seed kernel of the fruit, which account for 7% to 22% of the weight of the whole fruit . In Cameroon, a rapid estimation of this waste based on the national mango production, evaluated to 539,000 t in 2012 , showed that nearly 38,500 to 121,000 t of mango waste are produced. Environmental, hygienic, and public health problems result in unorganized management of this waste [3,4]. Valorization of mango seed kernels through production of butter and biofunctional flour are the main solutions technologically proposed, since different studies have shown that mango seed kernels contain various phenolic compounds. Mango seed kernel butter is used in cosmetics for its non-saponifiable matter content and its antioxidant activity potential [5-7]. The mango seed kernel flour displays interesting antioxidant and antibacterial properties [8-13]. These properties have also been found in mango peels . Valorizing this biowaste as a potential source of non-conventional oil and natural antioxidants represents an opportunity to improve mango producer's income, particularly in regions where poverty is current. In this respect, drying of the seed kernels is one of the main technological steps both for efficient extraction of the functional components and for inactivation of enzymatic degradation of the raw material. Drying of mango seed kernels before extraction contributes then to stabilize the product and to increase the yield of extraction. Since the temperature and the time of drying may affect the activity and the stability of bioactive compounds, due to chemical and enzymatic degradation, low evaporation, and/or thermal degradation, a badly carried out drying can lead to physicochemical reactions which can lead to losses of the textural and nutritional values  and thus bring damage in the quality of the product. Optimizing the drying of seed kernels would then enable to make sure that the product obtained has desired quality.
The present paper aims at determining the optimal drying parameters (temperature and time) for mango seed kernels in order to improve the butter extraction with preservation of its total phenolic compounds and antioxidant capacity. Response surface methodology is used in this respect, the optimization procedure consisting in determining the drying temperature and time which minimize moisture and maximize total polyphenol content, oil content, and antioxidant activity.
Mango (Local Ngaoundere) was collected from a farm in Ngaoundere (Adamawa region, Cameroon) at harvest stage maturity acceptable by consumers.
Acetone, n-hexane, tannic acid, methanol, trichloroacetic acid, and ascorbic acid were obtained from Sigma-Aldrich Chemie GmbH, Munich, Germany. Sodium carbonate and phosphate buffer solution was obtained from SERVA Electrophoresis GmbH, Germany. Folin-Ciocalteu, potassium hexacyanoferrate, and ferric chloride were from Fisher Scientific UK Ltd., Bishop Meadow Road, Loughborough, UK.
The mango flesh was removed, the seed sundried, and the seed kernels extracted manually using a stainless steel knife to open the shell. The seed kernels were then open in two cotyledons of about 9.7 ± 2.4 mm thickness for drying.
The drying process parameters considered for the study were drying temperature and drying time. Preliminary tests were allowed to fix the limits of these factors. The seed kernel cotyledons were laid on a tray and dried using fixed temperatures and times according to the central composite design (CCD), in a tropical oven dryer . The air velocity in the drying chamber was constant (0.55 ms−1), and the relative humidity of the chamber was 75 ± 3% (Hanna Instruments HI 8564 Thermo hygrometer, Hanna Instruments, Woonsocket, RI, USA). The dried seed kernels were reduced in powder form with knife mills (Retsch GM 200 GmbH, Retsch-Allee 1-542781 Haan, Germany) which are particularly suitable for grinding and homogenizing soft to medium-hard, elastic, fibrous, dry, or wet materials. It was assisted by a Retsch sieving shaker AS 300 permitted to obtain a particle size less than 1 mm. That powder was preserved in a plastic bag at 4°C in darkness until use.
Experimental design, modelling, validation of the model, and optimization
Central composite design: coded variables, real variables, and responses
Oil content (%)
Total polyphenols (% DM)
Antioxidant activity (eq g of VitC/100 g DM)
The coefficients of the models and statistical analysis (ANOVA) were obtained using the Minitab version 16 software (Minitab, Ltd., Brandon Court, Unit E1-E2 Progress Way, Coventry, CV3 2TE, UK), and the curves were plotted using Sigmaplot version 12.1 (Systat Software, Inc., 1735 Technology Drive, Suite 430, San Jose, CA 95110, USA).
Each linear, interaction, and quadratic contribution of each factor were obtained as follows:
Lastly, optimization was done using the software Mathcad 15.0 (build 220.127.116.116 Parametric Technology Corporation, 140 Kendrick Street, Needham, MA 02494, USA). The conditions fixed were to minimize moisture and total polyphenols and maximize oil content and antioxidant activity. The use of Sigmaplot version 12.1 (Systat Software, Inc., 1735 Technology Drive, Suite 430, San Jose, CA 95110, USA) permitted to draw the contour plots and superimpose the graphs in order to determine the optimal zone.
The determination of the moisture content was done using a standard method  and an isothermal oven (Heraeus, Type: T6, manufacturing no. 20001046, Kendro Laboratory Products, Langenselbold, Germany) for drying a known mass of the sample (using an electronic balance Gibertini no. 125186, made in Milan, Italy) at 105°C for 24 h. After cooling in a desiccator, the samples were reweighed (using an electronic balance Gibertini no. 125186, made in Milan, Italy). The water content (TE) representing the ratio of mass before and after heating in the oven is determined as a percentage.
The extraction of the lipid from seed kernels was made by the Soxhlet extraction method , using n-hexane as solvent. In practice, 600 g of dried mango seed kernel powder was used. The oil-hexane mixture obtained after extraction was separated on a rotary evaporator (Heidolph Salvis Electronic W 60) at 70°C to recover hexane. The oily extract, previously dried in an oven at 105°C for 20 min to evaporate residual n-hexane solvent, was then weighed. The lipid content was determined in g/100 g of dry matter (DM).
Determination of total phenolic compound
These analyses were based on the oxidation/reduction principle and employed Folin-Ciocalteu reagent . Briefly, 0.5 g of each sample was weighed in a glass beaker, and 10 mL of 70% acetone was added. The whole was stirred for 20 min at room temperature (25°C). The extract was centrifuged at 3,000 G for 10 min at 4°C. The supernatant was recovered and stored at 4°C. Volumes of 0, 10, 20, 30, 40, 50, 60, 70, 80, 90, and 100 μL of a tannic acid standard solution (0.1 mg/mL) for calibration curve and a suitable volume of extract were introduced into test tubes. The volumes were made up to 500 μL with distilled water. To these solutions, 250 μL of Folin-Ciocalteu reagent (1 N) and 1.25 mL of sodium carbonate (20%) were added. The mixtures were agitated and incubated at room temperature in the dark. After 40 min, the absorbance was read at 725 nm against the blank. The amount of total phenolic compound expressed by weight of tannic acid was then determined.
The antioxidant activity
To obtain the extract, 250 mg of powder was mixed in 25 mL of methanol at room temperature (25°C) for 2 h and centrifuged for 10 min at 4,000 G. The residue was extracted again with 25 mL of methanol and centrifuged (4,000 G) for 2 h again. The mixture of the two volumes of extract was then evaporated using a rotary evaporator to reduce the sample to 25 mL. This extract was recovered in sealed tubes and stored at 4°C until use. The antioxidant capacity of the different powder samples was assessed by determining their ability to reduce iron (III) to iron (II) .
In a test tube, 1 mL of each extract was mixed with 2.5 mL of a phosphate buffer solution (0.2 M, pH 6.6) and 2.5 mL of 1% potassium hexacyanoferrate [K3Fe(CN)6]. The whole was incubated for 30 min at 50°C in a water bath. Then, 2.5 mL of 10% trichloroacetic acid was added and the mixture was centrifuged for 10 min using a Heraeus Biofuge Primor, Kendro Laboratory Products, Langenselbold, Germany. After that, 2.5 mL of the supernatant was taken and mixed with 2.5 mL of distilled water and 0.5 mL of an aqueous solution of 0.1% FeCl3. The absorbance was read at 700 nm using a spectrophotometer Rayleigh VIS-723N (Beijing Beifen-Ruili Analytical Instrument (Group) Co., Ltd., Beijing, China). A calibration curve was done using ascorbic acid as reference at different concentrations. The total reducing power was expressed as equivalent of ascorbic acid (AAE).
Results and discussion
Model validation data
Y water(x 1, x 2)
Y oil(x 1, x 2)
Y polyphenols(x 1, x 2)
Y antioxidant(x 1, x 2)
Estimated coefficient impact and contributions to moisture, oil content, total polyphenols, and antioxidant activity
Effect of drying temperature
The impact of drying temperature (x 1) on physicochemical characteristics of Local Ngaoundere mango seed kernels was significant on the decrease of water content (P = 0.000), oil content (P = 0.033), and total polyphenols (P = 0.000), respectively (Table 3). Moreover, it had significant impact on the increase of antioxidant activity with P = 0.000 (Table 3).
Effect of drying time
The drying time (x 2) as a sole factor on some physicochemical characteristics of Local Ngaoundere mango seed kernels had a significant impact on the decrease of water content (P = 0.000) and had a significant impact on the increase of total polyphenols (P = 0.002) and antioxidant activity (P = 0.000), respectively (Table 3), while it had no significant impact on the increase or decrease of oil content with P = 0.104 (Table 3).
The effect of drying time on the moisture of mango seed kernels is shown in Figure 1b. It started from 23.2% w/w at 6 h and then decreased to a minimum value of 9.3% w/w at 55.68 h. After that, a slight increase until 10.3% w/w was observed at 69 h. This could be explained by the fact that when air held the maximum possible amount of vapor, the vapor exerted a saturation vapor pressure, and since the water vapor present was less than the maximum, the air took up more moisture. This evaporation which took place from the surface of the seed kernels induced moisture decrease, and it could be attained using time .
Although phenolic compounds are considered as heat-sensitive compounds , the increase in the total polyphenols as the drying time lengthened was observed (Figure 3b). In fact, it started from 1.20 mg/g at 6 h and then increased to 1.38 mg/g at 69 h. As far as this aspect is concerned, the literature shows contradictory results. But it could be explained by the sum of the content of the individual phenolic acids in the free fraction which significantly increased as the drying time lengthened as observed also for mandarin pomace .
The effect of drying time on antioxidant activity of mango seed kernels is shown in Figure 4b. It started from 500.58 mg AAE/100 g DM at 6 h and then increased to a maximum value of 753.64 mg AAE/100 g DM at 36.05 h. After that, it decreased until 449.71 mg AAE/100 g DM at 69 h. This result highlights the relationship between the previously observed enhancement of antioxidant potential and the increase in the content of some individual polyphenols. In fact, this could be explained by the fact that Maillard reaction products, which can be formed as a consequence of heat treatment or time, generally exhibit strong antioxidant properties [40-43].
Effect of interaction drying temperature/drying time
The impact of interaction of drying temperature/drying time (x 1 x 2) on some physicochemical characteristics of Local Ngaoundere mango seed kernels was not significant on water content (P = 0.924), oil content (P = 0.986), and total polyphenols (P = 0.137) (Table 3), while it was significant on the increase of antioxidant activity (P = 0.004) (Table 3).
The results obtained for the action of drying parameters (time and temperature) on water content, fats content, total polyphenols, and antioxidant activity on the basis of the models were optimized to determine satisfactory domains of compromise. These domains were obtained for four key physicochemical characteristics of mango seed kernels, by fixing them at water content ≤10% w/w , oil content ≥9% w/w, total polyphenols ≥1 mg/g, and antioxidant activity ≥1,000 mg AAE/100 g DM.
The effects of drying parameters (drying temperature and time) on some physicochemical characteristics of mango seed kernels were studied. Drying temperature was impacting more than the drying time. The interaction was only significant for antioxidant activity, meaning that there was no synergetic action of drying temperature and time for water, oil, and total polyphenol content. The study showed that satisfactory properties could be achieved when acting on drying parameters. Optimization of physicochemical properties of mango seed kernels showed that compromise could permit to obtain physicochemical characteristics of importance in order to assess the exploitability of the results for the valorization of mango seed kernels.
- A f :
analysis of variance
ascorbic acid equivalent
average absolute deviation
- B f :
- X 0i :
center of variable
central composite design
- R 2 :
coefficient of determination
- β ij :
coefficient of the interactions terms
- β i :
coefficient of the linear terms
- β ii :
coefficient of the quadratic terms
- x i :
coded variables given by the Doehlert table
- β 0 :
- Y i,exp :
- ∆X i :
- k 0 :
number of center points
- N :
number of experiments
- k :
number of variables
- P :
- X i :
- Y :
response surface methodology
- Y i,cal :
This work was undergone in the framework of the research group TQI2A (Technologie, Qualité et Innovations Agro Alimentaires) funded by AIRD (Agence Inter Etablissements de Recherche pour le Développement).
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