- Open Access
Valorization of fruits and vegetables waste through green extraction of bioactive compounds and their nanoemulsions-based delivery system
Bioresources and Bioprocessing volume 6, Article number: 26 (2019)
Fruits and vegetables, a significant segment of food sector, generate large volume of wastes annually. They constitute an excellent source of several valuable components (carotenoids, polyphenols, etc.), also known as bioactive compounds. These bioactive compounds have a positive impact on health and are known to modulate the metabolic processes as well as influence the cellular activities in the human health due to their antioxidant, anti-cancer, anti-inflammation, anti-allergenic and anti-atherogenic properties; depending upon the pathway and their bioavailability in the body. Despite this, some of these compounds are hydrophobic in nature and therefore are less bioavailable in the body. However, with the technological advancements like nanoemulsions, their solubility, stability and functional properties can be enhanced. This review provides the comprehensive information about the green extraction techniques and innovative delivery system such as nanoemulsions for bioactive compounds generated from fruits and vegetables waste.
Over the last decade, food processing industry has observed a tremendous growth and is one of the fastest growing sectors throughout the world. However, significant quantities of wastes are generated during the processing. According to the reports, approximately 20% of the wastes out of total 14 million metric tons (MMT) are generated during the processing of food and drink, its distribution and retail (Parfitt et al. 2010). In contrast to the other food processing sector, higher amounts of wastes, equivalent to 25–30%, are obtained from fruits and vegetables (F&Vs) industries that generally include peels, rind, seeds, core, rag, stones, pods, vine, shell, skin, pomace, etc. (Ajila et al. 2010). It was estimated that around 55 MMT waste is released during every processing steps, which includes processing of F&Vs (5.5 MMT), canning as well as freezing (6 MMT), wine processing (5–9 MMT) and others. The largest producers of these wastes are Philippines, China, India and the USA (FAO 2014; Schieber et al. 2001; Panouillé et al. 2007). These wastes, if not disposed or treated, cause serious environmental hazards due to the emission of various green house gases during decomposition (Vilariño et al. 2017). Hence, in order to circumvent these issues, there is a need for the valorization of wastes into various value-added products or extraction of bioactive compounds having various functional benefits.
Bioactive compounds or components can be defined as those food products or dietary supplements that have beneficial health effect on the host, besides meeting the basic nutritional requirements (Studdert et al. 2011). These are known to exhibit antioxidant, anti-microbial or anti-inflammatory activities on the host; however, these properties vary with their bioactivity, chemical structure, amount of dosage and others (Abdelkarim 2014). Being abundant in bioactive components, these can be extracted from wastes that can be further utilized for the development of functional foods. Different extraction techniques have been investigated for the value addition of wastes. Various compounds, such as carotenoids, phytosterols, omega-3 fatty acids, curcumin, oil-soluble vitamins, have been extracted using various techniques from wastes. However, due to the hydrophobicity and bioavailability of some bioactive compounds, there is a requirement of a potential delivery system, which may help in increasing their functionality to perform specific functions in the body.
In context to this, nanoemulsions have gained great attention and have been used as a delivery system for not only enhancing the stability, solubility as well as bioavailability of bioactive compounds in body, but also protecting them from adverse environmental conditions (pH, light, moisture, temperature, etc.). Nanoemulsions are used to reduce the separation of particles via sedimentation or creaming and improve the appearance (clear suspension) as well as other physico-chemical properties of food systems in which bioactive compounds are incorporated. The schematic representation of the extraction of different bioactive compounds from F&Vs wastes, delivery system and its applications thereof is depicted in Fig. 1. In view of the above, this review provides comprehensive information regarding the extraction of bioactive compounds from F&Vs waste and their improved delivery system for applications in food industries.
Bioactive compounds in fruits and vegetables waste
Fruits and vegetables are vital food commodities, which are required for fulfilment of the daily nutritional requirements of humans. In food industry, huge amount of waste is being produced that is characterized on the basis of biological oxygen demand as well as chemical oxygen demand. It is rich in different bioactive components; therefore, rather than considering as waste, these can be utilized for the recovery of valuable compounds, thereby leading to the zero-waste concept. Zero-waste concept is an effective strategy that allows effective valorization of the generated agro-industrial wastes to value-added products, which have several applications in food sector as colouring agents, antioxidative agents, preservatives and many more.
These wastes provide the wide array of bioactive compounds such as carotenoids (lutein and zeaxanthin), flavonoids (hesperetin, quercetin, genistein and kaempferol) and phenolic acids (Table 1). These phytochemicals vary in their polarity, solubility, molecular size, bioavailability and metabolic pathways. According to the Dietary Guidelines for Americans (2010), people should eat at least 4 serving of fruits and 5 serving of vegetables based on a 2000-kcal diet, but there is the huge difference between recommendation and consumption value (Liu 2013). The diet that constitutes large proportion of F&Vs has a significant role in reducing the risk of various diseases such as cardiovascular disease and cancer (Genkinger et al. 2004; Freedman et al. 2008). Generally, in F&Vs waste, the bioactive compounds with their natural antioxidant potency are higher than pulp. It indicates that F&Vs wastes also have good potential for natural antioxidants. These bioactive compounds can be added in the food either directly or by using different nanoencapsulation techniques such as nanoemulsions which further improve the functional value of these food products. Furthermore, these compounds can be used in nutraceutical, cosmetic and pharmaceutical industries.
Extraction of bioactive compounds by green technology
The bioactive compounds can be extracted by using both conventional and non-conventional technologies, each having their own advantages and disadvantages. However, in the recent years, green extraction technology, also known as non-conventional technology, has become the recent area of research. Owing to the high yield, reduced process time, high-quality products and less waste generation, these green technologies have replaced the traditional conventional technologies.
Various green technologies based on enzyme (enzyme-assisted extraction), pressurized liquid (pressurized liquid extraction), microwave (microwave-assisted extraction), ultrasound (ultrasound-assisted extraction), pulsed electric field (pulsed electric field assisted extraction) and supercritical fluid (supercritical fluid extraction) have been explored for the extraction of bioactive compounds. The effectiveness of these techniques varies with the properties of the source matrix, its chemical structure and process parameters like solvent, pressure, time and temperature (Azmir et al. 2013). Description of the various green extraction techniques is given in Table 2.
Supercritical fluid extraction
Extraction of compounds by this technique is carried out by using solvents above the critical point. This critical point can be defined as “the specific temperature (Tc) or pressure (Pc) point, above which gas and liquid do not exist as separate phase”. These fluids exhibit the properties of both liquids in terms of density and salvation power as well as gas (viscosity, diffusion and surface tension) that facilitates higher yield within short time (Ameer et al. 2017). The basic system for supercritical fluid extraction (SFE) includes mobile tank consisting of CO2, pump, solvent vessel, oven, controller and a trapping vessel (Azmir et al. 2013).
In contrast to the traditional methods, supercritical CO2 extraction, higher yields of naringin were obtained from citrus paradise using ethanol as a modifier (15% by weight) at the same process conditions, viz. 58.6 °C temperature and 9.5 MPa pressure (Giannuzzo et al. 2003). Further, extraction of phenolic compounds from rice wine lees had been investigated by both soxhlet extraction (SE) and SFE. In contrast to SE, SFE technique was found to be efficient in terms of reduced extraction time (1 h as compared to 6 h while using SE) and less ethanol requirement (1/10th of the quantity used in SE), thereby resulting in maximum yield of 43% phenols (Wu et al. 2009).
Carbon dioxide is the most commonly used solvent in food sector since it is safe and critical conditions are easily attainable (30.9 °C and 73.8 bars). However, the major limitation lies in its low polarity, which can be improved by using polar solvents (methanol, ethanol, dichloromethane, acetone, etc.) acting as modifiers that improve its solvating power and enhance its extraction efficiency by minimizing the interactions between the analyte and the matrix. Apart from this, low diffusibility of the solvent into the matrix, extended extraction time, high-pressure requirement, expensive infrastructure, variation in terms of consistency and reproducibility during continuous process are some of the other major limitations that hinder the scalability of this technique (Ameer et al. 2017).
The range of 20 to 2000 kHz is generally applied for ultrasound-assisted extraction (UAE). It is a low-cost and simple technique that is based upon two facts: one being the diffusion across the cell walls and other being the rinsing of the contents after cell disruption. It works on the principle of cavitation in which the waves cause the expansion and compression of the matrix that causes permeabilization of the cell wall and results in an increased yield of desired product. Several studies have revealed other possible mechanisms of UAE, which includes intensification of mass transfer, breakdown of the particles, increase in the accessibility of the solvents towards the cells. This technique has been widely used for the processing of liquid–liquid or liquid–solid samples. However, the efficiency of this technique is influenced by several factors such as pressure, temperature, frequency as well as time of sonication (Kentish and Feng 2014; Shen et al. 2017).
Amidst all, frequency is one of the major factors that have a profound effect not only on the yield, but also on the properties of the compound. It has been reported that extraction using more than 20 kHz energy affected the physico-chemical properties of the phytochemicals with the formation of free radicals (Shen et al. 2017; Kentish and Feng 2014).
The influence of the UAE technique on the extraction of tannins from Avaram shell (Cassia auriculata) at 100 W was studied by Sivakumar et al. (2014). In contrast to the magnetic stirring, UAE resulted in the improvement of the yield by 160% at 100 W. This may be attributed due to the improved mass transfer of cell components and leaching of tannins by ultrasound. Similarly, the yield of phenolic acids by UAE was 64.28 µg/g (caffeic acid), 1513 µg/g (ferulic acid) and 140 µg/g (p-coumaric acid), etc., which was higher than in the conventional maceration extraction, for example, 31.70 µg/g (caffeic acid), 763 µg/g (ferulic acid) and 63.1 µg/g (p-coumaric acid), etc. Extraction at higher temperature for prolonged time has a negative impact on the yield of phenolic compounds from citrus peels (Ma et al. 2008). Apart from this, the comparative study of maceration and UAE technique indicated that maceration (72 h) took more time than UAE (1 h) for extraction of phenolic compounds present in Punica granatum fruits (Chanfrau and Armas 2014). Furthermore, extraction of polysaccharides from black mulberry fruit resulted in highest yield of 3.13% that depicted the efficiency of the ultrasonic technique (Chen et al. 2015).
In contrast to conventional solvent techniques, UAE has certain advantages in terms of low temperature, which results in less thermal degradation of bioactive compounds and reduced extraction time. Overall, it is a cost-effective method at laboratory scale; however, in order to evaluate the process cost, there is a need for the scalability of this technique (Virot et al. 2010). In food industry, UAE is generally being used for different applications, such as sterilization, extraction and preservation (Zhang et al. 2018).
Microwave-assisted extraction (MAE) method involves the electromagnetic radiations, transmitted as waves in the frequency range from 300 MHz to 300 GHz, among which the common frequency used is 2450 MHz that is equivalent to almost 600–700 Watts energy. This technique is based on the principle that energy absorbed during the passage of microwaves through the medium converts into the thermal energy, which facilitates the processing (Zhang et al. 2011).
This technique results in the disruption of the hydrogen bonds, when a solvent placed in contact with the sample is heated, which results in the dipole rotation of the molecules and migration of the ions that allow the diffusion of the solvent, thus leading to the dissolution of the components (Datta et al. 2005). Other probable mechanisms involves the evaporation of the moisture within the cells, which induces high pressure on the cell wall and therefore changes the physical properties of the materials as well as modifies the porosity of biomaterials. This increases the penetration of the solvent, which further improves the yield of biomaterials (Routray and Orsat 2011).
Extraction of flavonoids from Terminalia bellerica plant using microwave resulted in the maximum yield of 82.74%, which was quite higher than that of conventional method where flavonoids content was 63.75% (Krishnan and Rajan 2016). MAE is an endothermic and spontaneous process; therefore, the operating conditions, such as temperature as well as solvent and feed ratio, have a strong influence on the yield of the flavonoids. Further, hesperidin yield was found to be 47.7 mg/g (86.8%) from the skin of citrus unshiu fruit by microwave-assisted extraction (Inoue et al. 2010). During extraction, temperature had a profound effect on the yield, since extraction above 140 °C temperature decreased the hesperidin content due to the interference of the other solubilized substances that inhibited the formation of hesperidin crystals. Moreover, in contrast to the mature peels, immature peels have about 3.1 times higher hesperidin content. The highest yield of phenolic compounds from chokeberries was 420.1 equivalents mg gallic acid/100 g obtained at 300 W for 5 min (Simić et al. 2016). Moreover, extraction of silibinin from Silybum marianum using MAE was conducted and the highest extract yield was 97.3% which was higher than in the conventional extraction techniques (Dhobi et al. 2009). Similarly, phenolic compounds from apple pomace have also been extracted and different parameters like solubility, dielectric constant, dissipation factor (δ) and solvent had a significant effect on yield (Chandrasekar et al. 2015). Furthermore, Alupului et al. (2012) had reported the higher recovery of flavonoids by MAE (74%) as compared to conventional solvent extraction (70.5%) technique and indicated that MAE method is efficient than the other techniques.
In the recent years, MAE has gained tremendous attention and is considered one of the excellent green extraction techniques due to the enhanced productivity, reduced extraction time, less solvent requirement, simplicity and low set-up costs, thereby reducing the overall cost of the process (Moreira et al. 2017).
Pulsed electric field
Pulsed electric field (PEF) is a non-thermal process that allows the application of direct current. In this technique, high-voltage pulses are passed through the materials placed within the electrodes for short time, usually in the range of microseconds to milliseconds. In this, when an electric current passes through the suspension of cells, cell structure is destroyed and thus the molecules separate according to their respective charge (Bryant and Wolfe 1987). This method can be operated in both modes: either in a batch or in a continuous mode (Puértolas et al. 2010). Various factors, such as field strength, energy, pulse number, properties of the materials and temperature, affect the extraction yield and therefore must be considered while designing a process (Heinz et al. 2003).
PEF has been used for the extraction of phenolic compounds and anthocyanins from Merlot skin (Delsart et al. 2012). Moreover, application of PEF on grape skin before maceration not only improves the stability of bioactive compounds during vinification, but also shortens the time of extraction (López et al. 2008). In contrast to the untreated control sample, PEF treatment along with the maceration technique (carried out during vinification) not only improved the colour as well as anthocyanin content but also resulted in an enhanced polyphenol content. Therefore, the application of PEF treatment during wine-making process could reduce the maceration time during vinification and also help in the improvement of wine quality. Further, the yield of phenolic compounds from orange peel was 159% by using PEF treatment at power of 7 kV/cm (Luengo et al. 2013).
The yield of phenolic compounds (102.86 mg GAE 100 g−1 FW) and flavonoid compounds (37.58 mg QE 100 g−1 FW) from onion was enhanced significantly by 2.2 and 2.7 times, respectively, as compared to control samples. It is an environmentally friendly process that can be used at large scale for the extraction of high-valued bioactive compounds (Liu et al. 2018). Onions are rich in phenols and flavonoids; thus, PEF is one of the most suitable non-thermal methods for the extraction of these valued components without a significant loss in their property. The electric field intensity and extraction time had a significant effect on the yield of both phenolic and flavonoid compounds. The occurrence of cytomembrane in the cell wall of plant tissue affects the migration of the intracellular substances between the cells. Hence, PEF is the promising strategy for the extraction of bioactive compounds, since it causes the disintegration of the cytomembrane in the tissues, which changes the permeability properties and increases the mass transfer across the cells, thereby resulting in higher yields.
This technology not only enhances the rate of extraction of anthocyanins, betanines, carotenoids, etc., from natural food sources and their by-products, but also lessens the time and solvent quantity and/or lower temperature values of extraction (Boussetta et al. 2012; Puértolas et al. 2013). The most remarkable feature of this technology is that being non-thermal in nature, it does not affect the quality of the final product. Moreover, it can be used for continuous processes either in pilot scale or at industrial level. Thus, it is one of the cost-effective emerging technologies that can be used in food processing sectors for large-scale processes.
It is counted as the novel technology and is considered as an alternative to conventional technique for extraction of various valuable components due to the use of water as solvent rather than organic chemicals (Puri et al. 2012). This technique is generally used when the phytochemicals are scattered in the cell cytoplasm and the presence of hydrogen or hydrophobic bonds in the polysaccharide-lignin network, thus posing a difficulty in extraction by conventional techniques. Despite this, because of its sustainable, eco-friendly nature and higher yield of the product, this technique has received more attention in last few years (Puri et al. 2012).
Enzyme concentration, composition, particle size, water-to-solid ratio and hydrolysis time are some of the major factors that influence the enzyme-assisted extraction of various bioactive compounds (Puri et al. 2012). This technique has been explored for the extraction of various components, such as carotenoids from pumpkin (Ghosh and Biswas 2015), anthocyanins from crocus sativus (Lotfi et al. 2015) and anthocyanin from grape skin. (Muñoz et al. 2004). Further, the yield of phenolics extracted from grape mare seeds was found to be 18–20 mg/g through pectinase (Štambuk et al. 2016), antioxidants phenols (0.152 mg/g) from apple pomace by Pectinex® (Oszmiański et al. 2011) and phenolics concentration (86.6%) from grape residues using Celluclast® (Gómez-García et al. 2012). Therefore, this extraction technique is very effective to enhance the recovery of compounds by using enzymes such as pectinase, cellulase and protease. However, cost of the enzyme is the limitation of this technique that hinders the scale-up process and further limits the yield of the final product. However, the cost can be reduced by immobilization technique that facilitates the reusability of the enzyme without loss in its specificity and activity (Puri et al. 2012).
Nanoemulsions-based delivery system for bioactive compounds and their applications in food systems
In today’s scenario, consumption of healthy and nutritive food ingredients having functional properties is prerequisite in our diet. With changing pattern of living style of people, focus has been shifted towards the nanotechnology. Nanotechnology has numerous applications in different sectors, and nanoemulsions-based delivery system has potential applications for the effective delivery of functional compounds in food sector.
Nanoemulsions are the mini-emulsions which have the particle size in the range of 100 nm. Nanoemulsions are kinetically stable, of small droplet size (high surface area per unit volume), optically transparent but thermodynamically unstable and act as the improved delivery system for different applications. It involves the dispersion of oil droplets in the aqueous phase along with suitable emulsifier, having droplet size in the range of 20–100 nm. If nanoemulsions are formed without using an emulsifier, the system immediately undergoes the different mechanisms such as Ostwald ripening, flocculation, gravitational separation and coalescence (McClements and Rao 2011). There are different approaches to design the nanoemulsions, and it depends upon different factors such as desirable end product, total energy requirement and storage conditions. Nanoemulsions are obtained by two main approaches called high energy and low energy as indicated in Table 3.
These nanoemulsions have different physico-chemical and functional properties depending upon their compositional ingredients and method of formation (low-energy or high-energy methods). Low-energy methods used for formation of nanoemulsions are spontaneous emulsification, phase inversion point, membrane emulsification, emulsion inversion point and solvent displacement. Nanoemulsions are formed by different mechanisms such as surface tension gradient, condensation mechanism, interfacial turbulence, dispersion mechanism and diffusion of solutes between two phases in spontaneous emulsification method.
Phase inversion point is the method in which transitional phase inversion takes place along with temperature change (thermal) at fixed composition. In this method, nanoemulsions formation takes place over a wide range of temperatures instead of fixed temperature along with energy saving and having the capability to involve the heat-sensitive compounds for food different applications while in emulsion inversion point, it undergoes phase inversion during emulsification process by changing the composition at constant temperature (isothermal). Membrane emulsification is the low-energy method which includes the formation of nanoemulsions by passing the dispersed phase through the membrane into a continuous phase. In the solvent displacement, the nanoemulsions form by rapid diffusion of the organic solvent in the aqueous phase and finally remove the organic phase under reduced pressure. All low-energy methods are simple and do not demand expensive equipments, but they require high surfactant-to-oil ratios (SORs) (Komaiko and McClements 2016). However, these low-energy techniques are potentially cost-effective strategy as compared to high-energy methods.
At present, applications of low-energy methods in food industries are limited due to the use of excessive amounts of synthetic surfactants than proteins or polysaccharides as emulsifiers. Some authors have also prepared nanoemulsions by spontaneous emulsification (Guttoff et al. 2015; An et al. 2014) and by emulsion inversion point (Hategekimana and Zhong 2015; Mayer et al. 2013).
High-energy approaches include high-pressure homogenization, ultrasonification and high-speed homogenization (Komaiko and McClements 2016). These methods for formation of nanoemulsions use the high intense force to rupture the large droplets into small ones by using the mechanical devices such as high-pressure homogenizers, microfluidizers, sonicators and high-speed devices (rotor/stator devices) (Kaci et al. 2018; Dey et al. 2018; Katsouli et al. 2018; Luo et al. 2017). This approach depends on many factors for obtaining the smallest size of the droplets such as operating conditions, homogenizer type and composition of a sample and physico-chemical properties of the different phases. In high-energy approach, less amount of SORs is used to form nanoemulsions as compared to the low-energy techniques. Nanoemulsions formed by high-energy techniques may be prone to Ostwald ripening but have better stability for coalescence and flocculation (McClements and Rao 2011). It can be controlled by adding compositional quantity such as ripening inhibitor. High-energy methods are more suitable in food industry because they can produce food grade nanoemulsions without using high SORs along with a wide range of different compositional entities (oil and emulsifier types) (McClements and Rao 2011). Therefore, according to their applicability, these techniques are used for the formation of nanoemulsions. High- and low-energy approaches have their own advantages and disadvantages.
The various applications of nanoemulsions in food sector include encapsulation of poorly soluble compounds (natural preserving agents, nutraceuticals, colourants, flavours), texture modification as well as enhancement in bioavailability, digestibility and solubility, etc. The nanoencapsulation of eugenol gave the highest nanoencapsulated yield as well as efficiency of 91.74 and 97.81%, respectively. Further, it was also found that this nanoencapsulated essential oil improved the antioxidant and anti-microbial activity in foods (Khaled et al. 2014). Some industries have applied the nanoemulsions on their products to increase their functional value in different aspects such as nanoencapsulation of phytosterols (Shemen, Haifa, Israel) and functional compounds (Aquanova, Germany), natural colourant (Wild flavours and Specialty Ingredients Inc., USA), and development of low fat products (Martins et al. 2007; Unilever 2011).
Application of nanoemulsions in food systems has certain challenges in terms of production, its characterization, product safety, acceptance and economy of the process that needs to be addressed (Salem and Ezzat 2019; Khaled et al. 2014).
Food safety and quality are the major concerns of food applications. To fulfil these aspects, nanotechnology sector is concentrating on the essential oils which have an anti-microbial activity associated with their phenolic compounds. Numerous authors were reported, regarding the nanoemulsions which contain the essential oils (lemongrass, thyme, clove, geranium, tea tree, rosewood, marjoram, mint, palmarosa, sage) in their composition (Salvia-Trujillo et al. 2015). It is mainly believed that nanoemulsions that contained lipophilic anti-microbials would be able to improve bactericidal effect by penetrating into microbial membranes more easily (Salvia-Trujillo et al. 2015). Therefore, these essential oils containing nanoemulsions are the good alternative to synthetic additives in terms of better anti-microbial, anti-fungal and anti-viral activity that further improves the shelf life (Shaaban et al. 2012). However, further research is required for correlating the relationship between the anti-microbial activities with different properties of nanoemulsions.
Delivery of poorly soluble drugs
Nanoemulsions are used for encapsulating the bioactive compounds which include β-carotene, lutein, quercetin, curcumin, resveratrol, capsanthin and vitamin E because these systems are well known to enhance the bioactivity, solubility, kinetic and physical stability (Surh et al. 2017; Sari et al. 2015; Kaur et al. 2016; Mao et al. 2009; Donsì et al. 2011; An et al. 2014; Hategekimana and Zhong 2015).
Modification of texture
Apart from delivery of bioactive compounds, these nanoemulsions have wide scope for modification of texture in food industries. Food industries like Unilever reduced the fat content (16% to 1%) of ice cream through nanoemulsions. In nanoemulsions, phenomenon of gelation is possible at low concentration of fat as compared to the emulsions. This property could be effective in food industries for producing the products like dressings, mayonnaise, etc., along with their desirable texture and flavour. However, it is required to focus on more researches which are related to edible systems and use their different functions for developing the new products and processes in food industry.
Conclusion and future prospective
Fruits and vegetables processing industry produces huge amount of waste that is rich in bioactive compounds. These bioactive compounds can be further utilized as natural colouring, antioxidant, preservative and anti-microbial agents in different industries. By using nanoencapsulation techniques such as nanoemulsions, the functional and textural properties of these bioactive compounds can be enhanced in food systems. Extraction of bioactives from wastes has been carried out using various non-conventional techniques; however, there is need for the development of novel and efficient techniques for the recovery of bioactive compounds. Among all, ultrasound-assisted extraction has significant applications in terms of higher yield, desirable quality, simple process and environmental friendly nature. In food processing sector, nanoemulsions are important delivery system for different bioactive compounds, which perform various functions such as increase their bioavailability, control the release of the ingredient, modulate the product texture and protect the ingredient from degradation in a better way. Although an intensive research is being carried on different extraction techniques and delivery system loaded with bioactive compounds, further research is required for better understanding for the specific function of bioactive compounds in particular food-based delivery system and its bioavailability in the body. A more comprehensive understanding is desirable for the bioaccessibility, metabolism and absorption of the encapsulated compound due to the alterations in their properties by taking place through the gastrointestinal tract. An extensive research should be done on the loading capacity of bioactive compounds encapsulated in nanoemulsions and their release characteristics. Therefore, it is critical to reduce the environmental pollution by extracting the bioactive compounds from fruits and vegetables waste which can further replace the synthetic antioxidant in various industries.
Availability of data and materials
fruits and vegetables
million metric tonnes
supercritical fluid extraction
pulsed electric field
gallic acid equivalents
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