Articles

The synthesis of biodiesel catalyzed by Mucor miehei lipase immobilized onto aminated polyethersulfone membranes

Nowadays, biodiesel has become more attractive in the quest for a novel fuel source due to its environmental benefits and the biodegradable properties. Transesterification of Helianthus annuus (sunflower) seeds o...

Authors: Nurrahmi Handayani, Deana Wahyuningrum, Muhammad Ali Zulfikar, Santi Nurbaiti, Cynthia L. Radiman and Buchari

Characterization of a novel metallocarboxypeptidase from Streptomyces cinnamoneus TH-2

Carboxypeptidases are exopeptidases that catalyze the release of amino acids from the C-terminus of peptides or proteins. The peptides consisting of hydrophobic amino acids taste bitter. Therefore, the hydroly...

Authors: Kun Wan, Misugi Uraji, Jiro Arima and Tadashi Hatanaka

Palm oil mill effluent as a low-cost substrate for bioflocculant production by Bacillus marisflavi NA8

Palm oil mill effluent (POME) generated from the palm oil milling process contains high organic matter from which the molecule of interest for bioconversion such as fermentable sugar can be derived. This study...

Authors: Bukhari Nurul-Adela, Abu-Bakar Nasrin and Soh-Kheang Loh

Bioformulation of Xenorhabdus stockiae PB09 for controlling mushroom mite, Luciaphorus perniciosus Rack

Bioformulations are the preparations that contain beneficial microorganisms as active ingredients and they may represent a novel alternative to be used in crop protection because of their safety to humans and ...

Authors: Piyarat Namsena, Prapassorn Bussaman and Paweena Rattanasena

Evaluation of gas supply configurations for microbial product formation involving multiple gaseous substrates

Gaseous substrates such as O₂ and CO₂ are often required in fermentation processes. However, a simple methodology to compare different gas supply strategies using gaseous substrates from different sources is miss...

Authors: Erik B. G. Häusler, Luuk A. M. van der Wielen and Adrie J. J. Straathof

Thanks to peer reviewers

Authors: Jian-He Xu

Concentration and characterization of microalgae proteins from Chlorella pyrenoidosa

Many methods are available for the concentration of proteins; however, most are not easily scalable due to costs, the need of specialized instruments and skilled workers or are very time-consuming. Three-phase...

Authors: Ashish G. Waghmare, Manoj K. Salve, Jean Guy LeBlanc and Shalini S. Arya

Characterization of biochars produced from peanut hulls and pine wood with different pyrolysis conditions

Application of modern biomass pyrolysis methods for production of biofuels and biochar is potentially a significant approach to enable global carbon capture and sequestration. To realize this potential, it is ...

Authors: James W. Lee, Bob Hawkins, Michelle K. Kidder, Barbara R. Evans, A. C. Buchanan and Danny Day

Microwave-assisted synthesis of silver nanoparticles from Origanum majorana and Citrus sinensis leaf and their antibacterial activity: a green chemistry approach

Silver nanoparticles (SNPs) play important role in the field of optics and electronics and also as a novel antibacterial agents. Here, we report a simple and green method for the biosynthesis of SNPs using aqu...

Authors: Darshan Singh, Deepti Rawat and Isha

Ameliorating effects of chicken feathers in plant growth promotion activity by a keratinolytic strain of Bacillus subtilis PF1

Feathers are the major byproducts of poultry industry and considered as waste. Feathers (composed of protein keratin) are metabolized by a number of microorganisms as a source of carbon and nitrogen. Degradati...

Authors: Khushboo Bhange, Venkatesh Chaturvedi and Renu Bhatt

Nanosilver-decorated nanographene and their adsorption performance in waste water treatment

Water, a precious resource for all living organisms has become a diminishing source in the present world. Reuse of water is a way of combating the scarcity of water. The remedy for the polluted water through e...

Authors: M. Jannathul Firdhouse and P. Lalitha

Optimization of different culture conditions for enhanced laccase production and its purification from Tricholoma giganteum AGHP

In current times, enzyme-catalyzed reactions have gained importance for the development of new chemical processes. These require the production of large quantity of enzyme at low cost. Solid-state fermentation...

Authors: Helina Patel and Akshaya Gupte

Bioremediation of Cu(II) contaminated water by Saccharum officinarum: effect of oxalic acid modification on equilibrium, kinetic and thermodynamic parameters

Copper is one of the heavy metals whose presence in aquatic environment in higher concentration poses a major threat to the environment. This is due to their toxic effects on the plants, animals and human heal...

Authors: Abideen Idowu Adeogun, Mopelola Abidemi Idowu, Kayode Olubunmi Akiode and Sikiru Akiyeye Ahmed

Asymmetric bio-epoxidation catalyzed with the styrene monooxygenase from Pseudomonas sp. LQ26

Styrene monooxygenases (SMOs) can catalyze the asymmetric epoxidation of alkenes to obtain optically active epoxides. This account describes a series of work of our group on the isolation, application, and imp...

Authors: Yan Liu, Yu-Chang Liu and Zhong-Liu Wu

Solid-state production of cellulase by Melanoporia sp. CCT 7736: a new strain isolated from coconut shell (Cocos nucifera L.)

The use of residues in industrial processing is expanding due to their low cost and abundant availability. Coconut shell is generated in large amounts in tropical areas due to the fruit processing and the coco...

Authors: Simone Lopes do Rêgo de Oliveira, Tatiane Cavalcante Maciel, Soraya de Oliveira Sancho and Sueli Rodrigues

Synergistic antibacterial and antibiofilm activity of silver nanoparticles biosynthesized by lignin-degrading fungus

The fabrication of silver nanoparticles (Ag-NPs) through green chemistry is an emerging area in the field of medical nanotechnology. Ag-NPs were fabricated by enzymatic reduction of AgNO₃ using two lignin-degradi...

Authors: Anand Barapatre, Keshaw Ram Aadil and Harit Jha

Degradation and detoxification of synthetic dyes and textile industry effluents by newly isolated Leptosphaerulina sp. from Colombia

Wastewaters from the textile industry are an environmental problem for the well-known Colombian textile industry. Ligninolytic fungi and their enzymes are an option for the treatment of these wastewaters; howe...

Authors: Jersson Plácido, Xiomara Chanagá, Santiago Ortiz-Monsalve, María Yepes and Amanda Mora

Responses of CHO-DHFR cells to ratio of asparagine to glutamine in feed media: cell growth, antibody production, metabolic waste, glutamate, and energy metabolism

Optimization of chemically defined medium has been a critical way to produce monoclonal antibody. Usually, amount of glutamine was added into the feed medium, but a half of asparagine was added. Our study foun...

Authors: Li-xiang Zhang, Wei-yan Zhang, Chen Wang, Jin-tao Liu, Xian-cun Deng, Xu-ping Liu, Li Fan and Wen-song Tan

Development and evaluation of a functional bioreactor for CO fermentation into ethanol

In a conventional syngas fermentation process, gas was released into the fermentation broth through a single orifice or multiple orifices, except the hollow fiber membrane reactor. Consequently, a simplified b...

Authors: Poritosh Roy, Animesh Dutta and Sheng Chang

Optimizing the preparation conditions and characterization of a stable and recyclable cross-linked enzyme aggregate (CLEA)-protease

Cross-linked enzyme aggregate (CLEA) is considered as an effective technique in the production of immobilized biocatalysts for its industrially attractive advantages. Simplicity, stability, low cost, time savi...

Authors: Safa Senan Mahmod, Faridah Yusof, Mohamed Saedi Jami and Soofia Khanahmadi

Aspergillus oryzae lipase-catalyzed synthesis of glucose laurate with excellent productivity

As nonionic surfactants derived from naturally renewable resources, sugar fatty acid esters (SFAEs) have been widely utilized in food, cosmetic, and pharmaceutical industries.

Authors: Xiao-Sheng Lin, Kai-Hua Zhao, Qiu-Ling Zhou, Kai-Qi Xie, Peter J. Halling and Zhen Yang

Bioprocess modelling for the design and optimization of lignocellulosic biomass fermentation

Lignocellulosic feedstocks, which are currently under-exploited, can be used for the production of biofuels, such as ethanol, and for biorefinery applications to produce a variety of value-added products. Alth...

Authors: Pornkamol Unrean

Current applications of different type of aqueous two-phase systems

In recent year, aqueous two-phase system (ATPS) has become a proven tool used in separation and purification technology. The application of ATPSs in clarification, partitioning and partial purification of biom...

Authors: Yee Koon Yau, Chien Wei Ooi, Eng-Poh Ng, John Chi-Wei Lan, Tau Chuan Ling and Pau Loke Show

A statistical approach on optimization of exopolymeric substance production by Halomonas sp. S19 and its emulsification activity

An exopolymer producing bacterial strain was identified as Halomonas sp. S19 by 16S rRNA gene sequencing isolated from Mandapam, Southeast coast of India. Strain S19 produces a significant amount of exopolymer (...

Authors: Parthiban Karuppiah, Vignesh Venkatasamy, Nilmini Viswaprakash and Thirumurugan Ramasamy

Biosynthesis of nanoparticles and silver nanoparticles

In this century, the development of nanotechnology is projected to be the establishment of a technological evolutionary of this modern era. Recently, nanotechnology is one of the most active subjects of substa...

Authors: Cheah Liang Keat, Azila Aziz, Ahmad M Eid and Nagib A. Elmarzugi

High solid saccharification using mild alkali-pretreated rice straw by hyper-cellulolytic fungal strain

The aim of this study was to use traditional mutagenesis to generate hyper-cellulolytic mutants with emphasis on stable, non-spore formers, shorter enzyme producing times and higher saccharification efficiency...

Authors: Garima Dixit, Amita R. Shah, Datta Madamwar and Madhuri Narra

Supercritical fluid extraction of polyphenols from lees: overall extraction curve, kinetic data and composition of the extracts

The increasing incidence of degenerative diseases has attracted the interest in the obtaining of bioactive compounds. Since seeds and skins from grapes are important sources of polyphenols which have been asso...

Authors: Angela M. Farías-Campomanes, Mauricio A. Rostagno, Joel J. Coaquira-Quispe and M. Angela A. Meireles

Identification of a new thermostable and alkali-tolerant α-carbonic anhydrase from Lactobacillus delbrueckii as a biocatalyst for CO₂ biomineralization

Carbonic anhydrase (CA, EC 4.2.1.1), an ancient enzyme and the fastest among many enzymes, is a useful biocatalyst for carbon capture use and storage (CCUS). The use of alkaline buffers and high temperatures a...

Authors: Chun-Xiu Li, Xiao-Chen Jiang, Yong-Juan Qiu and Jian-He Xu

Using the mycelium-covered cereals as an efficient inoculation method for rapamycin fermentation in a 15-L fermenter using Streptomyces hygroscopicus

Rapamycin is produced from Streptomyces hygroscopicus, and was initially identified as an antifungal antibiotic. More recently, rapamycin has been found to have various medical applications, including in relation...

Authors: Hong-Wei Yen and Meng-Hua Chiang

Biodegradation of malachite green by a novel copper-tolerant Ochrobactrum pseudogrignonense strain GGUPV1 isolated from copper mine waste water

Malachite green (MG) is a triphenyl methane cationic dye which is used to color fabrics and is employed as food additive, food coloring agent and medical disinfectant. MG is found to be toxic to aquatic organi...

Authors: Venkatesh Chaturvedi and Pradeep Verma

Model-based optimization of Scheffersomyces stipitis and Saccharomyces cerevisiae co-culture for efficient lignocellulosic ethanol production

The utilization of both C₆ and C₅ sugars is required for economical lignocellulosic bio-based processes. A co-culture system containing multiple strains of the same or different organisms holds promise for conver...

Authors: Pornkamol Unrean and Sutamat Khajeeram

Enhancement of the enzymatic hydrolysis of fines from recycled paper mill waste rejects

A significant fraction of short fibers commonly called “reject fines” is produced while recycling wastepaper at paper mills producing linerboard. These fines are usually rejected into the solid waste stream th...

Authors: Byeong C. Min, B. V. Bhayani, V. S. Jampana and B. V. Ramarao

Marked enhancement of Acinetobacter sp. organophosphorus hydrolase activity by a single residue substitution Ile211Ala

The activity of organophosphorus hydrolase (OPH) that catalyzes the hydrolysis of neurotoxic organophosphates (OPs) was reported to evolve from lactonase.

Authors: Jie Chen, Xiao-Jing Luo, Qi Chen, Jiang Pan, Jiahai Zhou and Jian-He Xu

Bioprospecting thermostable cellulosomes for efficient biofuel production from lignocellulosic biomass

The adverse climatic conditions due to continuous use of fossil-derived fuels are the driving factors for the development of renewable sources of energy. Current biofuel research focuses mainly on lignocellulo...

Authors: Richa Arora, Shuvashish Behera, Nilesh Kumar Sharma and Sachin Kumar

Optimization of bioconversion process for trehalose production from enzymatic hydrolysis of kudzu root starch using a visualization method

Trehalose has many advantages due to its inertness and the ability to stabilize biomolecules. Trehalose synthase can catalyze intramolecular rearrangement of the inexpensive maltose into trehalose in a single ...

Authors: Xin Yang, Liying Zhu, Ling Jiang, Qing Xu, Xian Xu and He Huang

Effect of voltage and electrode material on electroflocculation of Scenedesmus acuminatus

Microalgae are a promising new source for biomass production. One of the major challenges in regards to cost effectiveness is the biomass harvest. High energy input is required for the separation of the small ...

Authors: Franziska Bleeke, Gunnar Quante, Dominik Winckelmann and Gerd Klöck

Use of combined UV and chemical mutagenesis treatment of Aspergillus terreus D34 for hyper-production of cellulose-degrading enzymes and enzymatic hydrolysis of mild-alkali pretreated rice straw

Microbial production of cellulose-degrading enzymes could be significantly improved using traditional mutagenesis treatment. Development of high-titre cellulase producing mutants drastically reduces the costs ...

Authors: Adepu K Kumar, Bhumika S Parikh, Surendra P Singh and Deval Shah

Fetal bovine serum: geographic origin and regulatory relevance of viral contamination

The article aims to clarify an existing misunderstanding by the users of fetal bovine serum (FBS), who assume that certain countries, like Australia and New Zealand, have fewer cattle disease viruses and pose ...

Authors: Percy William Hawkes

Preliminary investigations on a polygalacturonase from Aspergillus fumigatus in Chinese Pu’er tea fermentation

Polygalacturonase is one kind of pectinases which hydrolyze the alpha-1,4 glycosidic bond between galacturonic acid residue. Polygalacturonase has been widely used in the fields of food, biofuel, and textile i...

Authors: Shihui Wang, Zhongshuai Lian, Liuyang Wang, Xiao Yang and Yun Liu

Rapid and efficient bioconversion of chicory inulin to fructose by immobilized thermostable inulinase from Aspergillus tubingensis CR16

Fructose, a monosaccharide, has gained wide applications in food, pharmaceutical and medical industries because of its favourable properties and health benefits. Biocatalytic production of fructose from inulin...

Authors: Sneha Trivedi, Jyoti Divecha, Tapan Shah and Amita Shah

Bacterial chitinase: nature and perspectives for sustainable bioproduction

Concurrent advances in a number of fields have fostered the development of bioprocesses for biochemical production. Ideally, future bioprocesses will meet the demands of commercial chemical markets in an econo...

Authors: Qiang Yan and Stephen S Fong

Comparison of steam-assisted versus microwave-assisted treatments for the fractionation of sweet sorghum bagasse

The fractionation of lignocellulosic materials can be applied to get each of the components (cellulose, hemicellulose and lignin) of biomass in its maximum purity and yield. The individual component can be fur...

Authors: Jiby Kudakasseril Kurian, Yvan Gariepy, Valerie Orsat and G. S. Vijaya Raghavan

Secretive expression of heterologous β-glucosidase in Zymomonas mobilis

Zymomonas mobilis is an efficient ethanol fermentation strain, but its narrow substrate range limits its fermentation in lignocellulose hydrolysate. As a potential consolidated bioprocessing (C...

Authors: Zichen Luo and Jie Bao

Cultural optimization and metal effects of Shewanella xiamenensis BC01 growth and swarming motility

Shewanella species belonging to dissimilatory metal bacteria were found to decolorize most textile dyes and had also attracted great interests in regard to bioremediation. However, studies have...

Authors: I-Son Ng, Chukwuma Isaac Ndive, Yunli Zhou and Xiaomin Wu

Preparation of ursodeoxycholic acid from 7-ketone lithocholic acid by stereoselective electroreduction

Ursodeoxycholic acid (UDCA) is an important clinical drug in the treatment of liver disease. In previous work, ursodeoxycholic acid was prepared by traditional organic synthesis. The preparation of ursodeoxych...

Authors: Xiaomei Huang and Xuejun Cao

Wet explosion pretreatment of loblolly pine leads to an increase in methoxylation of the lignin

In biorefineries, various pretreatments traditionally employ hazardous chemicals (ammonia, sulfuric acid, sulfite, etc.) for opening the softwood structure and to facilitate easy accessibility of the cellulose...

Authors: Diwakar Rana, Dhrubojyoti D. Laskar, Keerthi Srinivas and Birgitte K. Ahring

Partitioning of thermostable glucoamylase in polyethyleneglycol/salt aqueous two-phase system

A major challenge in downstream processing is the separation and purification of a target biomolecule from the fermentation broth which is a cocktail of various biomolecules as impurities. Aqueous two phase sy...

Authors: Vinayagam Ramesh and Vytla Ramachandra Murty

Disposal of municipal solid waste with in situ termireactors: proof-of-concept

Termites, which are among the nature’s most effective scavengers and earthmovers, are distinguished by their ability to process lignin. In this report, arguably the first of its type, we present studies in whi...

Authors: SA Abbasi and S Gajalakshmi

Ligninolytic enzymes: a biotechnological alternative for bioethanol production

Ligninolytic fungi and enzymes (i.e., laccase, manganese peroxidase, and lignin peroxidase) have been applied recently in the production of second-generation biofuels. This review contains the analysis of lign...

Authors: Jersson Plácido and Sergio Capareda

UV mutagenesis treatment for improved production of endoglucanase and β-glucosidase from newly isolated thermotolerant actinomycetes, Streptomyces griseoaurantiacus

Bioconversion of cellulosic biomass into fuel ethanol involves several steps, among which enzymatic breakdown of cellulose into fermentable sugars play a significant role. The key enzymes involved in cellulosi...

Authors: Adepu K. Kumar