Articles

Efficient expression of sortase A from Staphylococcus aureus in Escherichia coli and its enzymatic characterizations

Sortase A (SrtA) is a transpeptidase found in Staphylococcus aureus, which is widely used in site-specific protein modification. However, SrtA was expressed in Escherichia coli (E. coli) in rather low level (rang...

Authors: Zhimeng Wu, Haofei Hong, Xinrui Zhao and Xun Wang

Techno-economic analysis of extraction-based separation systems for acetone, butanol, and ethanol recovery and purification

Dual extraction, high-temperature extraction, mixture extraction, and oleyl alcohol extraction have been proposed in the literature for acetone, butanol, and ethanol (ABE) production. However, energy and econo...

Authors: Víctor Hugo Grisales Díaz and Gerard Olivar Tost

Advances in industrial microbiome based on microbial consortium for biorefinery

One of the important targets of industrial biotechnology is using cheap biomass resources. The traditional strategy is microbial fermentations with single strain. However, cheap biomass normally contains so co...

Authors: Li-Li Jiang, Jin-Jie Zhou, Chun-Shan Quan and Zhi-Long Xiu

Changes of membrane fatty acids and proteins of Shewanella putrefaciens treated with cinnamon oil and gamma irradiation

In order to detect the antimicrobial mechanism of combined treatment of cinnamon oil and gamma irradiation (GI), the membrane fatty acids and proteins characteristics of Shewanella putrefaciens (S. putrefaciens) ...

Authors: Fei Lyu, Fei Gao, Qianqian Wei and Lin Liu

A food-grade expression system for d-psicose 3-epimerase production in Bacillus subtilis using an alanine racemase-encoding selection marker

Food-grade expression systems require that the resultant strains should only contain materials from food-safe microorganisms, and no antibiotic resistance marker can be utilized. To develop a food-grade expres...

Authors: Jingqi Chen, Zhaoxia Jin, Yuanming Gai, Jibin Sun and Dawei Zhang

An efficient multi-stage fermentation strategy for the production of microbial oil rich in arachidonic acid in Mortierella alpina

Fungal morphology and aeration play a significant role in the growth process of Mortierella alpina. The production of microbial oil rich in arachidonic acid (ARA) in M. alpina was enhanced by using a multi-stage ...

Authors: Wen-Jia Wu, Ai-Hui Zhang, Chao Peng, Lu-Jing Ren, Ping Song, Ya-Dong Yu, He Huang and Xiao-Jun Ji

Recent updates on different methods of pretreatment of lignocellulosic feedstocks: a review

Lignocellulosic feedstock materials are the most abundant renewable bioresource material available on earth. It is primarily composed of cellulose, hemicellulose, and lignin, which are strongly associated with...

Authors: Adepu Kiran Kumar and Shaishav Sharma

Fungal pretreatment of raw digested piggery wastewater enhancing the survival of algae as biofuel feedstock

Understanding about the impact of white rot fungi on indigenous bacterial communities, NH₄ ⁺ and turbidity in digested piggery wastewater, will allow the optimization of wastewater trea...

Authors: Junying Liu, Wen Qiu and Yunpu Wang

Hydrolysis of carotenoid esters from Tagetes erecta by the action of lipases from Yarrowia lipolytica

The present study was conducted to evaluate the feasibility of enzymatic hydrolysis of carotenoid esters from Tagetes erecta using lipases from the yeast of Yarrowia lipolytica, with the aim of obtaining free lut...

Authors: Abraham Figueiras Abdala, Alfonso Pérez Gallardo, Lorenzo Guevara Olvera and Eleazar Máximo Escamilla Silva

Investigation of spiral-wound membrane modules for the cross-flow nanofiltration of fermentation broth obtained from a pilot plant fermentation reactor for the continuous production of lactic acid

The separation performance of seven polymer membranes for the nanofiltration of sodium lactate in fermentation broth was investigated. Each module was introduced into the test stand, and the system curve was o...

Authors: Hendrik Laube, Roland Schneider and Joachim Venus

Online flow cytometry, an interesting investigation process for monitoring lipid accumulation, dimorphism, and cells’ growth in the oleaginous yeast Yarrowia lipolytica JMY 775

This study aims to understand and better control the main biological mechanisms and parameters modulating the various phenomena affecting Yarrowia lipolytica JMY775 and its lipids accumulation. The results obtain...

Authors: Doria Naila Bouchedja, Sabine Danthine, Tambi Kar, Patrick Fickers, Abdelghani Boudjellal and Frank Delvigne

Stereoselective biotransformation of racemic mandelic acid using immobilized laccase and (S)-mandelate dehydrogenase

(S)-Mandelate dehydrogenase (SMDH) and laccase were immobilized on chitosan. The bi-enzymatic system with immobilized SMDH and immobilized laccase was taken to catalyze the stereoselective transformation of ra...

Authors: Xing Chen, Chengli Yang, Peng Wang, Xuan Zhang, Bingxin Bao, Dali Li and Ruofu Shi

Dissolved oxygen control strategy for improvement of TL1-1 production in submerged fermentation by Daldinia eschscholzii

2,3-Dihydro-5-hydroxy-2-methylchromen-4-one (TL1-1) is a phenolic compound with significant anti-fungal and anti-cancer activities produced by Daldinia eschscholzii (D. eschscholzii). However, studies have rarely...

Authors: Xing-chen Wei, Liu Tang and Yan-hua Lu

Degradation study of lindane by novel strains Kocuria sp. DAB-1Y and Staphylococcus sp. DAB-1W

This study was carried out to isolate and characterize the bacterial strains from lindane-contaminated soil and they were also assessed for their lindane-degrading potential.

Authors: Dharmender Kumar, Abhijit Kumar and Jyoti Sharma

Metabolic engineering of Serratia marcescens MG1 for enhanced production of (3R)-acetoin

Optically pure acetoin (AC) is an important platform chemical which has been widely used to synthesize novel optically active α-hydroxyketone derivatives and liquid crystal composites.

Authors: Xin Lv, Lu Dai, Fangmin Bai, Zhanqing Wang, Liaoyuan Zhang and Yaling Shen

Engineering a wild-type diploid Saccharomyces cerevisiae strain for second-generation bioethanol production

The cost-effective production of second-generation bioethanol, which is made from lignocellulosic materials, has to face the following two problems: co-fermenting xylose with glucose and enhancing the strain’s...

Authors: Hongxing Li, Yu Shen, Meiling Wu, Jin Hou, Chunlei Jiao, Zailu Li, Xinli Liu and Xiaoming Bao

Electricity generation from rice bran in microbial fuel cells

Rice bran is a by-product of the rice milling process and mostly discarded in Japan. Although many studies have shown that microbial fuel cells (MFCs) are able to generate electricity from organic wastes, limi...

Authors: Shu Takahashi, Morio Miyahara, Atsushi Kouzuma and Kazuya Watanabe

Penicillin acylase-catalyzed synthesis of N-bromoacetyl-7-aminocephalosporanic acid, the key intermediate for the production of cefathiamidine

Enzymatic approaches have become promising alternatives to chemical methods for the production of semi-synthetic β-lactam antibiotics. In this work, enzymatic synthesis of N-bromoacetyl-7-aminocephalosporanic aci...

Authors: Xiao-Li Zhang, Min-Hua Zong and Ning Li

In silico profiling of cell growth and succinate production in Escherichia coli NZN111

Succinic acid is a valuable product due to its wide-ranging utilities. To improve succinate production and reduce by-products formation, Escherichia coli NZN111 was constructed by insertional inactivation of lact...

Authors: Xingxing Jian, Ningchuan Li, Cheng Zhang and Qiang Hua

Combined ¹³C-assisted metabolomics and metabolic flux analysis reveals the impacts of glutamate on the central metabolism of high β-galactosidase-producing Pichia pastoris

Pichia pastoris is a popular recombinant protein expression system for its accessibility of efficient gene manipulation and high protein production. Sufficient supply of precursors, en...

Authors: Ping Liu, Mingzhi Huang, Menglei Guo, Jiangchao Qian, Weilu Lin, Ju Chu, Yingping Zhuang and Siliang Zhang

Protein expression analysis of a high-demeclocycline producing strain of Streptomyces aureofaciens and the roles of CtcH and CtcJ in demeclocycline biosynthesis

Streptomyces aureofaciens strain A6-9, obtained with traditional mutagenesis, produces elevated levels of 6-DCT. The increased formation of 6-DCT may be attributable to the changes in ...

Authors: Hong Li, Ruifang Ye, Guizhen Lin, Deyu Zhu and QuanGui Mao

Improvement of chaetominine production by tryptophan feeding and medium optimization in submerged fermentation of Aspergillus fumigatus CY018

Chaetominine (CHA) is a novel alkaloid with excellent medicinal activities produced by Aspergillus fumigatus CY018. However, its further application has been severely restricted by the low production yield. In th...

Authors: Yu-peng Zhang, Rui-hua Jiao, Yan-hua Lu and Ling-yun Yao

Exploring productive sequence space in directed evolution using binary patterning versus conventional mutagenesis strategies

Recent methodology development in directed evolution of stereoselective enzymes has shown that various mutagenesis strategies based on saturation mutagenesis at sites lining the binding pocket enable the gener...

Authors: Zhoutong Sun, Pamela Torres Salas, Elina Siirola, Richard Lonsdale and Manfred T. Reetz

Enhanced arachidonic acid production using a bioreactor culture of Mortierella alpina with a combined organic nitrogen source

The organic nitrogen source is one of the key factors affecting Mortierella alpina cell growth as well as arachidonic acid (ARA) production. The aim of the present work is to achieve an optimized recipe of organi...

Authors: Xue-ping Ling, Si-yu Zeng, Cui-xue Chen, Xiao-ting Liu and Ying-hua Lu

Isolation and characterization of glycolipid biosurfactant produced by a Pseudomonas otitidis strain isolated from Chirimiri coal mines, India

Biosurfactants (BSs) are amphipathic, surface active molecules produced by microorganisms and can reduce the surface tension and interfacial tension. The present study emphasizes the isolation and structural c...

Authors: Pallavi Singh and Bhupendra N. Tiwary

Preliminary integrated economic and environmental analysis of polyhydroxyalkanoates (PHAs) biosynthesis

Being biodegradable and renewable, polyhydroxyalkanoates (PHAs), a green polymer, attract much attentions as potential alternative for conventional plastics due to increased concern towards environmental issue...

Authors: Yoong Kit Leong, Pau Loke Show, Heng Chou Lin, Chih Kai Chang, Hwei-San Loh, John Chi-Wei Lan and Tau Chuan Ling

Isolation, characterization, and application of biosurfactant by Klebsiella pneumoniae strain IVN51 isolated from hydrocarbon-polluted soil in Ogoniland, Nigeria

Considerable attention has been given to the use of biosurfactants in recent times because of their potential industrial and environmental applications and ecological friendliness. Hydrocarbon-polluted soils h...

Authors: Ijeoma Vivian Nwaguma, Chioma Blaise Chikere and Gideon Chijioke Okpokwasili

Effects of hot-washing process on structure and enzymatic hydrolysis of treated steam explosion corn stover

Cellulase adsorption of lignocellulosic materials is the key link during enzymatic hydrolysis. Hot-washing process (above lignin glass transition temperature) was used to change the physical structure of ligni...

Authors: Xu Yang, Xiaojian Ma, Hongliang Li, Junyin Chen and Shuqi Fang

Microbial fuel cell: a green approach for the utilization of waste for the generation of bioelectricity

Today we are witnessing a global energy crisis due to huge energy demands and limited resources. Non-renewable energy sources are depleting and renewable energy sources are not properly utilized. There is an i...

Authors: Venkatesh Chaturvedi and Pradeep Verma

Regulation of porcine circovirus type 2-like particles expressed in baculovirus expression system

Porcine circovirus type 2 (PCV2) virus-like particles (VLPs) are an effective vaccine against post-weaning multisystemic wasting syndrome. Burst sequence (BS) and the chicken 5′-HS4 β-globin (HS4) insulator we...

Authors: Yingjun Li, Xiaoping Yi, Yingping Zhuang and Ju Chu

Erratum to: Characterization of biochars produced from peanut hulls and pine wood with different pyrolysis conditions

Authors: James W. Lee, Bob Hawkins, Michelle K. Kidder, Barbara R. Evans, Sokwon Paik, A. C. Buchanan and Danny Day

Cultivation of Nannochloropsis sp. using narrow beam angle light emitting diode in an internally illuminated photobioreactor

This paper reports on the growth condition of Nannochloropsis sp. in an annular column-type photobioreactor (PBR) using light-emitting diode as an internal illumination.

Authors: Mohamad Taisir, Chee Loong Teo, Ani Idris and Affendi M. Yusuf

Investigating the mechanism of nanofiltration separation of glucosamine hydrochloride and N-acetyl glucosamine

Glucosamine hydrochloride (GAH) and N-acetyl glucosamine (NAG) are chitin derivatives. Owing to their excellent biological activity, they have long been used as pharmaceuticals and nutraceuticals. However, both o...

Authors: Shengnan Zhang, Jiachun Zhou, Liqiang Fan, Yongjun Qiu, Lihua Jiang and Liming Zhao

Enhancing total fatty acids and arachidonic acid production by the red microalgae Porphyridium purpureum

This study investigated the effect of aeration rate and light intensity on biomass production and total fatty acids (TFA) accumulation by Porphyridium purpureum. The red microalgae is also known to accumulate con...

Authors: Gaomin Su, Kailin Jiao, Jingyu Chang, Zheng Li, Xiaoyi Guo, Yong Sun, Xianhai Zeng, Yinghua Lu and Lu Lin

Sequence similarity network analysis, crystallization, and X-ray crystallographic analysis of the lactate metabolism regulator LldR from Pseudomonas aeruginosa

The FadR subfamily of regulators plays essential roles in the regulation of diverse metabolic pathways in bacteria. LldR, an FadR-type regulator, regulates lactate utilization in Pseudomonas aeruginosa.

Authors: Bo Xin, Geng Wu, Kunzhi Zhang, Yongxing He, Hongzhi Tang, Chao Gao, Ping Xu and Cuiqing Ma

Synthesis and characterization of crystalline carboxymethylated lignin–TEOS nanocomposites for metal adsorption and antibacterial activity

Biodegradable carboxymethylated lignin–tetra ethoxysilane (TEOS) nanocomposites (CML–T) were synthesized using lignin extracted from rice straw (RS) followed by surface modification through carboxymethylation....

Authors: Kumari Shweta and Harit Jha

Optimization of milk-clotting enzyme production by Bacillus amyloliquefaciens SP1 isolated from apple rhizosphere

Present study aims to isolate and optimize fermentation conditions of milk-clotting enzyme producing rhizobacteria Bacillus amyloliquefaciens SP1.

Authors: Shiwani Guleria, Abhishek Walia, Anjali Chauhan and C. K. Shirkot

Lactic acid production from paper sludge by SSF with thermotolerant Rhizopus sp.

Rhizopus fungi is suitable for the production of lactic acid, which is the backbone material of polylactic acid used as green plastic from lignocellulosic biomass, since it can grow an...

Authors: Maki Takano and Kazuhiro Hoshino

Current status of cow dung as a bioresource for sustainable development

Cow dung, an excreta of bovine animal, is a cheap and easily available bioresource on our planet. Many traditional uses of cow dung such as burning as fuel, mosquito repellent and as cleansing agent are alread...

Authors: Kartikey Kumar Gupta, Kamal Rai Aneja and Deepanshu Rana

Synthesis and characterization of Acacia lignin-gelatin film for its possible application in food packaging

The aim of the present investigation was to develop Acacia lignin-gelatin (LG) blended films using glycerol as plasticizer and to establish correlation between lignin contents and structure, thermal and mechanica...

Authors: Keshaw Ram Aadil, Anand Barapatre and Harit Jha

Production of bioethanol by bacterial co-culture from agro-waste-impacted soil through simultaneous saccharification and co-fermentation of steam-exploded bagasse

The production of bioethanol by co-culture of cellulolytic and xylanolytic bacteria isolated from agro-waste-impacted soil through simultaneous saccharification and co-fermentation (SSCF) of steam-exploded bag...

Authors: Francis Sopuruchukwu Ire, Victor Ezebuiro and Chimezie Jason Ogugbue

Palm kernel oil-based polyester polyurethane composites incorporated with multi-walled carbon nanotubes for biomedical application

In this study, polyurethane (PU) films from palm kernel oil-based polyester (PKO-p) incorporated multi-walled carbon nanotubes (MWNTs) were prepared via evaporative casting method. Nanoparticle fluid dispersio...

Authors: Nurul Nabilah bt Zulkifli, Khairiah bt Hj Badri and Khairul Anuar Mat Amin

Effect of methyl jasmonate and salycilic acid synergism on enhancement of bilobalide and ginkgolide production by immobilized cell cultures of Ginkgo biloba

Bilobalide and ginkgolides are reported to be present only in Ginkgo biloba. However, only trace amounts of bilobalide and ginkgolides are contained in the ginkgo leaves. Nowadays, there has been considerable int...

Authors: Agus Sukito and Sanro Tachibana

Conversion of residues and by-products from the biodiesel industry into value-added products

Biodiesel, one of the most important sources of renewable energy, is produced in large quantities around the world; however, its production generates different kinds of residues and by-products which raise eco...

Authors: Jersson Plácido and Sergio Capareda

The synthesis of biodiesel catalyzed by Mucor miehei lipase immobilized onto aminated polyethersulfone membranes

Nowadays, biodiesel has become more attractive in the quest for a novel fuel source due to its environmental benefits and the biodegradable properties. Transesterification of Helianthus annuus (sunflower) seeds o...

Authors: Nurrahmi Handayani, Deana Wahyuningrum, Muhammad Ali Zulfikar, Santi Nurbaiti, Cynthia L. Radiman and Buchari

Characterization of a novel metallocarboxypeptidase from Streptomyces cinnamoneus TH-2

Carboxypeptidases are exopeptidases that catalyze the release of amino acids from the C-terminus of peptides or proteins. The peptides consisting of hydrophobic amino acids taste bitter. Therefore, the hydroly...

Authors: Kun Wan, Misugi Uraji, Jiro Arima and Tadashi Hatanaka

Palm oil mill effluent as a low-cost substrate for bioflocculant production by Bacillus marisflavi NA8

Palm oil mill effluent (POME) generated from the palm oil milling process contains high organic matter from which the molecule of interest for bioconversion such as fermentable sugar can be derived. This study...

Authors: Bukhari Nurul-Adela, Abu-Bakar Nasrin and Soh-Kheang Loh

Bioformulation of Xenorhabdus stockiae PB09 for controlling mushroom mite, Luciaphorus perniciosus Rack

Bioformulations are the preparations that contain beneficial microorganisms as active ingredients and they may represent a novel alternative to be used in crop protection because of their safety to humans and ...

Authors: Piyarat Namsena, Prapassorn Bussaman and Paweena Rattanasena

Evaluation of gas supply configurations for microbial product formation involving multiple gaseous substrates

Gaseous substrates such as O₂ and CO₂ are often required in fermentation processes. However, a simple methodology to compare different gas supply strategies using gaseous substrates from different sources is miss...

Authors: Erik B. G. Häusler, Luuk A. M. van der Wielen and Adrie J. J. Straathof

Thanks to peer reviewers

Authors: Jian-He Xu